Cytoprotective effect against UV-induced DNA damage and oxidative stress: Role of new biological UV filter

Said, Toihiri; Dutot, Mélody; Martin, Chantal; Beaudeux, Jean-Louis; Boucher, Cyril; Enee, E.; Baudouin, Christophe; Warnet, Jean‐Michel; Rat, Patrice

doi:10.1016/j.ejps.2006.11.001

Cited by 46 publications

(39 citation statements)

References 43 publications

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“…The effects of UVR-A and UVR-B (290-400 nm) are considered particulary detrimental (32). At a molecular level, UVR is associated with the creation of active free radicals which attack and deactivate various macromolecules (33). Free radicals may in turn be deactivated by tear film proteins, including lactoferrin (34).…”

Section: Concepts Of Inheritancementioning

confidence: 99%

Pathogenetic mechanisms and treatment options for ophthalmic pterygium: Trends and perspectives (Review)

Detorakis

Spandidos²

2009

Int J Mol Med

152

103

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Abstract. Pterygium is a fibrovascular lesion of the ocular surface that can display an aggressive clinical behavior and, occasionally, threaten vision. Although there is no consensus on its pathogenesis, recent evidence suggests that it is a proliferative, rather than degenerative condition strongly correlated with exposure to ultraviolet radiation (UVR) of solar light. Molecular genetic alterations reported in association with pterygium include loss of heterozygosity (LOH), point mutations of proto-oncogenes, such as K-ras and alterations in the expression of tumor suppressor genes, such as p53 or p63. Other findings in pterygium include the frequent detection of HPV DNA, ocular surface changes such as the overexpression of various proteins, including defensins and phospolipases D, as well as the up-regulation of growth factors, such as bFGF or VEGF. Although pterygium management has traditionally involved surgery, often enhanced by the use of antimetabolites, recent advancements in the understanding of molecular and biochemical events underlying pterygium pathogenesis may enable the use of less invasive treatment methods.

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Section: Concepts Of Inheritancementioning

confidence: 99%

Pathogenetic mechanisms and treatment options for ophthalmic pterygium: Trends and perspectives (Review)

Detorakis

Spandidos²

2009

Int J Mol Med

152

103

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“…This oil is already used in skin-healing creams at a concentration of 30%. We have also already shown that C. inophyllum oil has anti-UV and antioxidant properties [15]. …”

Section: Introductionmentioning

confidence: 99%

Ocular Burn: Rinsing and Healing with Ionic Marine Solutions and Vegetable Oils

et al. 2008

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Purpose: We investigated the effects of various rinsing and healing protocols on corneal wound repair and inflammation following alkali burn in rabbits. Methods: We conducted in vitro, in vivo and ex vivo studies. First, different rinse solutions were tested in vitro after incubation of ocular cells with methanol or NaOH. Cell viability was then assessed using the neutral red test (cytofluorometry). Second, NaOH was applied to rabbit corneas and associations of rinse solutions (NaCl 0.9% or controlled ionization marine solutions) with N-acetylcysteine or vegetable oils (from Calophyllum inophyllum and Aleurites moluccana) were tested in vivo. The regeneration of the corneal epithelium and the infiltration of inflammatory cells were evaluated using in vivo confocal microscopy and ex vivo histological cuts. Results: The association of a controlled ionization marine solution with 10% C. inophyllum oil and 90% A. moluccana oil induced regeneration of the corneal epithelium and a decrease in inflammatory cells. Conclusions: Irrigation with marine solution followed by treatment with a mixture of C. inophyllum and A. moluccana oils is a promising treatment for ocular burns.

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“…Cell viability was measured fluorometrically using Neutral Red dye (Borenfreund and Puerner, 1985;Eisenbrand et al, 2002;Creppy, 2004;Said et al, 2007;Dutot et al, 2008). Briefly, the cells were washed with PBS to remove the test agent and 5 mM Neutral Red solution was added to the control and the treated cells in 10 replicate wells (100 ml per well) in a 96-well plate and the culture plate was incubated for 10 min at 37°C in a saturating humidity atmosphere of 5% CO2 in air.…”

Section: Cell Viability Determined By Neutral Red Uptakementioning

confidence: 99%

“…Cellular oxidative stress was determined fluorometrically by the dichlorofluorescein (DCF) assay (Said et al, 2007;Shao et al, 2008). The non-fluorescent compound 2' ,7'-dihydrodichlorofluorescein diacetate (DHDCFDA) is taken up by the viable cells (Said et al, 2007).…”

Section: Cellular Oxidative Stressmentioning

confidence: 99%

Comparative hepatotoxicity of deoxynivalenol in rat, mouse and human liver cells in culture

Sahu

O’Donnell

Wiesenfeld

2010

J of Applied Toxicology

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The present study was undertaken to assess, in vitro, the hepatotoxic potential of the food-borne mycotoxin, deoxynivalenol (DON), using rat (Clone9 and MH1C1), mouse (NBL CL2) and human (WRL68 and HepG2) liver cells in culture. The cells were treated with DON for 24 h at 37 degrees C in 5% CO(2) at concentrations of 0-25 microg ml(-1). Following the treatment period, the cells were assayed for biochemical markers of hepatotoxicity that included three independent cytotoxicity assays, oxidative stress and mitochondrial dysfunction. Concentration-dependent cytotoxicity of DON was observed in each of the five different liver cells derived from three different species (rat, mouse and human) over the entire concentration range studied, beginning at 0.1 microg ml(-1). At these concentrations DON did not induce a biologically significant increase in oxidative stress in these liver cells, and showed a significant decrease in the mitochondrial function only in the rat liver MH1C1 cells compared with the control. The results of this in vitro study suggest that DON is a potential hepatotoxin for the rat, mouse and human liver cells in the concentration range tested in this study. The liver cells used in this study showed distinct endpoint-sensitivity to DON related to the species.

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Cytoprotective effect against UV-induced DNA damage and oxidative stress: Role of new biological UV filter

Cited by 46 publications

References 43 publications

Pathogenetic mechanisms and treatment options for ophthalmic pterygium: Trends and perspectives (Review)

Pathogenetic mechanisms and treatment options for ophthalmic pterygium: Trends and perspectives (Review)

Ocular Burn: Rinsing and Healing with Ionic Marine Solutions and Vegetable Oils

Comparative hepatotoxicity of deoxynivalenol in rat, mouse and human liver cells in culture

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