Cytosolic Invertase-Mediated Root Growth Is Feedback Regulated by a Glucose-Dependent Signaling Loop

Meng, Lai-Sheng; Zhang, Wei; Cao, Xiaoying; Chen, Tong; Lv, Meng-Jiao; Yu, Fei; Loake, Gary J.

doi:10.1104/pp.20.00778

Cited by 27 publications

(40 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a connection between trehalose formation and cell wall thickening as well as cell division (Gómez et al 2006). Glucose has an important role as signal molecule interacting with CINV1 regulating A. thaliana root growth (Meng et al 2020). Concerning all this, it is possible that at the early stage of the S. indica-host interaction two different processes take place.…”

Section: Discussionmentioning

confidence: 99%

Serendipita indica changes host sugar and defense status in Arabidopsis thaliana: cooperation or exploitation?

Opitz

Daneshkhah

Lorenz

et al. 2021

Planta

View full text Add to dashboard Cite

Main conclusion Manipulation of sugar metabolism upon S. indica root colonization triggers changes in sugar pools and defense responses in A. thaliana. Abstract Serendipita indica is an endophytic fungus that establishes mutualistic relationships with many different plants including important crops as well as the model plant A. thaliana. Successful root colonization typically results in growth promotion and enhanced tolerance against various biotic and abiotic stresses. The fungus delivers phosphorus to the host and receives in exchange carbohydrates. There are hints that S. indica prefers hexoses, glucose, and fructose, products of saccharose cleavage driven by invertases (INVs) and sucrose synthases (SUSs). Carbohydrate metabolism in this interaction, however, remains still widely unexplored. Therefore, in this work, the sugar pools as well as the expression of SUSs and cytosolic INVs in plants colonized by S. indica were analyzed. Using sus1/2/3/4 and cinv1/2 mutants the importance of these genes for the induction of growth promotion and proper root colonization was demonstrated. Furthermore, the expression of several defense-related marker genes in both multiple mutants in comparison to the wild-type plants was determined. Our results show that in colonized A. thaliana plants S. indica manipulates the sugar metabolism by altering the expression of host’s INV and SUS and modulates both the sugar pools and plant defense in its favor. We conclude that the interaction A. thaliana–S. indica is a balancing act between cooperation and exploitation, in which sugar metabolism plays a crucial role. Small changes in this mechanism can lead to severe disruption resulting in the lack of growth promotion or altered colonization rate.

show abstract

Section: Discussionmentioning

confidence: 99%

Serendipita indica changes host sugar and defense status in Arabidopsis thaliana: cooperation or exploitation?

Opitz

Daneshkhah

Lorenz

et al. 2021

Planta

View full text Add to dashboard Cite

show abstract

“…Plant materials and growth conditions pap-D mutant (CS3884) (Qi et al, 2011), myb75-1 (pst16228) (Bhargava et al, 2010), mir156a-2 (CS71699) (Yu et al, 2013;Yang et al, 2013), spl9-4 (Col-0) (Wu et al, 2009), CINV1pro-GUS (Meng et al, 2020), cinv2 (Sail_518_D02) (Barratt et al, 2009) were described previously.…”

Section: Discussionmentioning

confidence: 99%

“…To produce CINV1-LUC and SPL9-LUC, the target promoter was amplified via PCR, then inserted into the cloning site of the pGreen0800-LUC vector (Meng et al, 2020). See Table S1 for primer.…”

Section: Discussionmentioning

confidence: 99%

Glucose- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition

Meng

Bao

et al. 2021

Cell Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…The qPCR reactions of lncRNAs and target genes were conducted using the SYBR Premix Ex TaqTM II kit (TaKaRa, Japan). All the reactions were conducted using an ABI 7500 PCR machine according to the method ( Zhou et al, 2016 ; Meng et al, 2020 ). The SlActin gene was used as a control to normalize gene expression using the 2 –ΔΔ CT method.…”

Section: Methodsmentioning

confidence: 99%

Bacillus subtilis SL18r Induces Tomato Resistance Against Botrytis cinerea, Involving Activation of Long Non-coding RNA, MSTRG18363, to Decoy miR1918

et al. 2021

View full text Add to dashboard Cite

Mounting evidence has indicated that beneficial rhizobacteria can suppress foliar pathogen invasion via elicitation of induced systemic resistance (ISR). However, it remains elusive whether long non-coding RNAs (lncRNAs) are involved in the mediation of the rhizobacteria-primed ISR processes in plants. Herein, we demonstrated the ability of the rhizobacterial strain Bacillus subtilis SL18r to trigger ISR in tomato plants against the foliar pathogen Botrytis cinerea. Comparative transcriptome analysis was conducted to screen differentially expressed lncRNAs (DELs) between the non-inoculated and SL18r-inoculated plants. Among these DELs, four variants of MSTRG18363 possessed conserved binding sites for miR1918, which negatively regulates immune systems in tomato plants. The expression of MSTRG18363 in tomato leaves was significantly induced by SL18r inoculation. The transcription of MSTRG18363 was negatively correlated with the expression of miR1918, but displayed a positive correlation with the transcription of the RING-H2 finger gene SlATL20 (a target gene of miR1918). Moreover, MSTRG18363-overexpressing plants exhibited the enhanced disease resistance, reduction of miR1918 transcripts, and marked increases of SlATL20 expression. However, the SL18r-induced disease resistance was largely impaired in the MSTRG18363-silenced plants. VIGS-mediated SlATL20 silencing also greatly weakened the SL18r-induced disease resistance. Collectively, our results suggested that induction of MSTRG18363 expression in tomato plants by SL18r was conducive to promoting the decoy of miR1918 and regulating the expression of SlATL20, thereby provoking the ISR responses against foliar pathogen infection.

show abstract

Cytosolic Invertase-Mediated Root Growth Is Feedback Regulated by a Glucose-Dependent Signaling Loop

Cited by 27 publications

References 35 publications

Serendipita indica changes host sugar and defense status in Arabidopsis thaliana: cooperation or exploitation?

Serendipita indica changes host sugar and defense status in Arabidopsis thaliana: cooperation or exploitation?

Glucose- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition

Bacillus subtilis SL18r Induces Tomato Resistance Against Botrytis cinerea, Involving Activation of Long Non-coding RNA, MSTRG18363, to Decoy miR1918

Contact Info

Product

Resources

About