Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea)

Jimenez, Paula Christine; Wilke, Diego Veras; Takeara, Renata; Lotufo, Tito Monteiro da Cruz; Pessoa, Cláudia; Moraes, Manoel Odoríco de; Lopes, Norberto P.; Costa-Lotufo, Letı́cia V.

doi:10.1016/j.cbpa.2007.02.018

Cited by 33 publications

(21 citation statements)

References 31 publications

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“…Analysis of changes in cell morphology was evaluated using AO/EB fluorescence staining [15]. Briefly, the suspension of (+)-cyanidan-3-ol-treated cells, 4 µl/ml of dye mixture, containing 100 µg/ml acridine orange (Sigma, USA) and 100 µg/ml ethidium bromide (Sigma, USA) in PBS were added, and washed once with PBS.…”

Section: Methodsmentioning

confidence: 99%

Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular Carcinoma

et al. 2013

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The objective of this study was to evaluate the cytotoxicity of (+)-cyanidan-3-ol (CD-3) in human hepatocellular carcinoma cell line (HepG2) and chemopreventive potential against hepatocellular carcinoma (HCC) in Balb/c mice. The HepG2 cell line was treated with CD-3 at various concentrations and the proliferation of the HepG2 cells was measure by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB) and lactate dehydrogenase (LDH) assays. Cell apoptosis was detected by Hoechst 33258 (HO), Acridine orange/ethylene dibromide (AO/EB) staining, DNA fragmentation analysis and the apoptosis rate was detected by flow cytometry. The HCC tumor model was established in mice by injecting N-nitrosodiethylamine/carbon tetrachloride (NDEA/CCl4) and the effect of CD-3 on tumor growth in-vivo was studied. The levels of liver injury markers, tumor markers, and oxidative stress were measured. The expression levels of apoptosis-related genes in in-vitro and in vivo models were determined by RT-PCR and ELISA. The CD-3 induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes under fluorescent microscopy and DNA fragmentation analysis. Annexin V/PI assay demonstrated that apoptosis increased with increase in the concentration of CD-3. The expression levels of apoptosis-related genes that belong to bcl-2 and caspase family were increased and AP-1 and NF-κB activities were significantly suppressed by CD-3. Immunohistochemistry data revealed less localization of p53, p65 and c-jun in CD-3 treated tumors as compared to localization in NDEA/CCl4 treated tumors. Taken together, our data demonstrated that CD-3 could significantly inhibit the proliferation of HepG2 cells in-vitro and suppress HCC tumor growth in-vivo by apoptosis induction.

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Section: Methodsmentioning

confidence: 99%

Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular Carcinoma

et al. 2013

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“…The morphological changes of apoptosis were evaluated by fluorescence microscopy [13]. B16F10 melanoma cells (1x10 6 ) were cultured in coverslip in a small petri plate and allowed to grow for 24 h and another petri plate was kept as control.…”

Section: Ao-etbr Stainingmentioning

confidence: 99%

Lawsonia Alba Leaves Induce Apoptosis and Cell Cycle Arrest in B16f10 Melanoma Cells

Deb

Hansda

Dutta

et al. 2018

Int J Pharm Pharm Sci

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Objective: The present study was designed to investigate the anti-melanoma activity of the ethyl acetate fraction of Lawsonia alba lam leaves (ELA) against B16F10 cells.Methods: Cytotoxicity of ELA on B16F10 cells was determined by MTT assay and supported with the morphology of apoptotic and necrotic cells under phase-contrast microscope, fluorescence microscopy with AO/EtBr, confocal microscopy with PI, Agarose gel electrophoresis and Annexin V-FITC/PI staining, mitochondrial membrane potential and cell cycle arrest by FACS was also performed on B16F10 cells.Results: Cytotoxic effect of ELA on B16F10 melanoma cell was confirmed by MTT assay with an IC50 Conclusion: Ethyl acetate fraction of Lawsonia alba L. leaves possesses potent apoptotic activity against B16F10 cells.value of 14.10μg/ml. Morphological study showed arrays of both the nuclear changes including chromatin condensation and apoptotic body formation indicating that the treatment with ELA and 5-Fluorouracil (standard) causes apoptotic changes in the melanoma cells compared to the untreated control. Agarose gel electrophoresis study showed fragmented DNA in the form of ladder. The depolarization of mitochondrial membrane potential was confirmed. Flow cytometric analysis showed appreciable number of cells in early apoptotic stage. The cells were arrested mostly in G0/G1 phase of cell cycle.

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“…For example, Jimenez et al [26] found that dichloromethane extract from Eudistoma vannamei exerts significant cytotoxic effects. This extract was tested on HL-60 promyeloblastic leukemia cells, with results suggesting that cytotoxicity is related to initiation of apoptosis in these cells.…”

Section: Cytoxicitymentioning

confidence: 99%

Tunicates: A Vertebrate Ancestral Source of Antitumor Compounds

Cooper

Albert

2014

Handbook of Anticancer Drugs From Marine Origin

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An alarming increase in cancer deaths around the globe has sparked a quest for new effective antitumor drugs developed through biological screening of both terrestrial and marine organisms. Recently, analyses of marine-derived alkaloids isolated from tunicates, a close relative to vertebrates, reveal various anti-cancer activities including anti-angiogenic action, anti-proliferative activity, inhibition of key cellular events like topoisomerase function and tubulin polymerization with respect to cytotoxicity and apoptosis. Vast numbers of potential anticancer molecules in the marine biosphere cannot be underestimated. Tunicate compounds have already entered the market and shown significant success. Moreover, improved technology has enabled synthesis of these molecules by a diverse cadre of professionals including career biologists, immunobiologists, invertebrate immunologists, and marine biologists who use an amazing array of procedures. Although there are no guarantees of consistently reliable success, identifying and comprehending the basic nature of these compounds is a promising first step towards unique pharmaceutical designs that promise to provide a therapeutic solution.

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Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea)

Cited by 33 publications

References 31 publications

Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular Carcinoma

Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular Carcinoma

Lawsonia Alba Leaves Induce Apoptosis and Cell Cycle Arrest in B16f10 Melanoma Cells

Tunicates: A Vertebrate Ancestral Source of Antitumor Compounds

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