Cytotoxic and antimicrobial potential of different leaves extracts of R. fruticosus used traditionally to treat diabetes

Weli, Afaf M.; Al-Saadi, Hanady S.; Al-Fudhaili, Rouqaya S.; Hossain, Amzad; Putit, Zabida Binti; Jasim, M. K.

doi:10.1016/j.toxrep.2020.01.006

Cited by 21 publications

(15 citation statements)

References 24 publications

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“…The BSLA results for the highly toxic plant extracts correlate with results from other toxicity studies. The toxicity result of Croton tiglium (Euphorbaceae), whose LC 50 = 1.32 μg/mL, is consistent with the anticancer activity exhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA), a major active constituent of croton oil [ 201 ], although it is lower than the 30 μg/mL reported by Meyer et al in one of the pioneer studies using BSLA for active plant constituents [ 20 ]. The BSLA result of Arcangelesia flava , belonging to family Menispermeceae, is similar to the findings of Soonthornchareonnon and co-workers [ 202 ].…”

Section: Discussionsupporting

confidence: 62%

“…To assess the toxicity of the plants in parallel, we used the brine shrimp lethality assay (BSLA), which is a simple, reliable, inexpensive micro-scale bioassay technique that can detect a range of bioactivity in ethanolic extracts as well as isolated purified bioactive natural products [ 18 ]. This assay is based on the ability of the extract to kill laboratory-cultured brine shrimp nauplii, from the genus Artemia [ [19] , [20] , [21] ]. Brine shrimp lethality assay only requires small amounts of sample.…”

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confidence: 99%

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Comparative toxicity, phytochemistry, and use of 53 Philippine medicinal plants

2022

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Section: Discussionsupporting

confidence: 62%

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confidence: 99%

Comparative toxicity, phytochemistry, and use of 53 Philippine medicinal plants

2022

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“…Studies of the antimicrobial capacity of non-polar blackberry seed extracts are not reported in the literature. Weli et al [ 19 ] studied the antibacterial activity of blackberry leaf extracts (methanol, hexane, chloroform, ethyl acetate, and hydro-alcoholic) and concluded that they are effective against several Gram-positive and Gram-negative micro-organisms.…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition, Antioxidant, Antimicrobial and Cytotoxic/Cytoprotective Activity of Non-Polar Extracts of Grape (Vitis labrusca cv. Bordeaux) and Blackberry (Rubus fruticosus) Seeds

et al. 2021

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The aim of this study was to compare the influence of the extraction method, chemical composition, antimicrobial effects, antioxidant activity, and cytotoxicity on human cells of the non-polar extracts of grape (Vitis labrusca) and blackberry (Rubus fruticosus) seeds. The Soxhlet (Sox), Bligh–Dyer (BD), and ultrasound (US) methods were used for extractions. For blackberry non-polar seed extract, extraction via the BD method showed the highest mean values of total phenolic content (TPC), expressed in milligrams of gallic acid equivalent per 100 mL of non-polar seed extracts (102.37 mg GAE/100 mL), and higher antioxidant activity in relation to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, expressed in milligrams of gallic acid equivalent per 100 mL of non-polar seed extracts (11.50 mg AAE/100 mL), if compared with the Sox and US extractions. Similar results were obtained for the non-polar grape seed extracts, where BD extraction obtained the highest values for TPC (28.61 mg GAE/100 mL) and DPPH (35.36 mg AAE/100 mL). The type of extraction method had an impact on the composition of fatty acids. Only the non-polar blackberry and grape seed extracts obtained via the Sox method showed some in vitro inhibitory effect against Escherichia coli (IAL 2064) and Staphylococcus aureus (ATCC 13565). Regardless of the extraction method used, the non-polar blackberry and grape seed extracts did not decrease the cell viability (IC50 >1000 µg/mL) of cancer and normal cell lines, thus indicating the relative safety of the extracts. All the seed extracts decreased the generation of reactive oxygen species in the cell lines. Blackberry and grape seed lipid fractions can be utilized as antioxidants, and the extraction methods used cause significant changes in relation to their bioactivity and chemical composition.

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“…Therefore, given the high prevalence of chronic and erosive diseases, it necessitates using plants to provide the antioxidants needed by the body, especially plants that are high in phenol and flavonoids. Therefore, to provide the natural antioxidants needed by the body, the consumption of plants with high phenolic compounds is recommended (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Antimicrobial Activity of Medicinal Plant Extracts on Bacillus cereus Isolated from Soil

et al. 2021

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Background: Because many plant extracts have a significant inhibitory effect on pathogenic microorganisms, so we aimed to evaluate the antioxidant and antimicrobial activity of some plant extracts on Bacillus cereus isolated from soil. Methods: The chicory (Cichorium intybus L.), hyacinth (Hypericum perforatum L.), lavender (Lavandula angustifolia), yew (Taxus baccata), and thyme (Thymus vulgaris L.) plants were collected and identified in the botanical laboratory of the University of Zabol. To prepare the ethanolic extract, 40 g of dried leaves of plants were used in 400 cc of ethanol. Different strains of B. cereus used in this study were isolated from soil and identified by biochemical, bacteriological, and growth tests as well as standard tests. Antimicrobial effects were investigated by diffusion method in Müller Hinton agar medium using 6 mm paper discs according to Bauer and Kirby instructions as well as microdilution. Statistix ver10 software was used for statistical calculations. Mean comparisons were performed using the LSD at the 1% level, and Excel was also used to draw the figures. Results: The diameter of the growth inhibition zone of plant extracts against B. cereus at a dilution of 100 ppm was investigated, and it was found that different extracts had different effects on inhibiting the growth of B. cereus (P < 0.01). LSD test showed that thyme (T. vulgaris L.) had the highest (15 mm) effect on growth inhibition of strains 2 and 3 of B. cereus and the lowest (1 mm) effect on growth inhibition of strain 1 of B. cereus. The lowest MIC and MBC of thyme (T. vulgaris L.) against B. cereus samples were 3.1 and 6.2 ppm, respectively. Conclusions: Considering the side effects of chemical drugs and antibiotics, as well as the potential effect of medicinal plant extracts, especially T. vulgaris L. on B. cereus, it is recommended that T. vulgaris L. may inhibit the growth of B. cereus.

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Cytotoxic and antimicrobial potential of different leaves extracts of R. fruticosus used traditionally to treat diabetes

Abstract: Graphical abstract

Cited by 21 publications

References 24 publications

Comparative toxicity, phytochemistry, and use of 53 Philippine medicinal plants

Comparative toxicity, phytochemistry, and use of 53 Philippine medicinal plants

Chemical Composition, Antioxidant, Antimicrobial and Cytotoxic/Cytoprotective Activity of Non-Polar Extracts of Grape (Vitis labrusca cv. Bordeaux) and Blackberry (Rubus fruticosus) Seeds

Investigation of Antimicrobial Activity of Medicinal Plant Extracts on Bacillus cereus Isolated from Soil

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