Cytotoxic and genotoxic effects of fumonisin B1 on rabbit kidney RK13 cell line

Abstract

Fumonisins, mycotoxins produced by certain strains of Fusarium moniliforme, could induce various diseases in animals and are suspected human carcinogens. Fumonisin B1 (FB1), the most commonly found fumonisin, has been characterised as a tumour initiator and a tumour promoter, a mitogen and an anti-proliferative agent. In this study we examined the cytotoxicity and genotoxicity of FB1 in rabbit kidney RK13 cells. To evaluate the effects of FB1 on survival of this cell line we analysed cell viability, membrane integrity, DNA fragmentation and overall morphology of the cells. The genotoxic potential of FB1 was estimated by monitoring the ability of this mycotoxin to induce micronuclei in RK13 cells. Exposure to FB1 caused a significant increase in micronucleus frequency in a concentration- and in a time-dependent manner. Nanomolar concentrations of FB1 decreased cell viability after 24 h and even more so after 48 h of exposure. The morphological changes observed suggested that an increased number of RK13 cells were dying by the process of apoptosis. However, FB1 also induced impairments of cell and mitochondrial membrane integrity, as assessed by lactate dehydrogenase and glutamate dehydrogenase leakage. These results could imply that nanomolar concentrations of FB1 induced apoptosis, which subsequently may proceed to secondary necrosis. In summary, our observations suggest that FB1 is genotoxic and cytotoxic to RK13 cells.

Subchronic mycotoxicoses in Wistar rats: Assessment of the in vivo and in vitro genotoxicity induced by fumonisins and aflatoxin B1, and oxidative stress biomarkers status

“…Related to this, Norred et al (1992) reported that FB 1 could not be considered to be a genotoxic mycotoxin, when evaluated by its ability to induce unscheduled DNA synthesis (UDS) in primary rat hepatocytes. However, genetic damage was observed by other authors when the toxin was tested by its ability to induce MN in primary hepatocytes (Knasmuller et al, 1997), rabbit kidney RK13 cells (Rumora et al, 2002), human lymphocytes and Allium cepa (onion) (Lerda et al, 2005). Moreover, the FB 1 was also found to be genotoxic in vitro when it was evaluated by means of comet assay in rat astrocytes (Galvano et al, 2002a) and in human fibroblasts (Galvano et al, 2002b).…”

Effects ofFicus thonningii(Blume) [Morarceae] Stem-Bark Ethanolic Extract on Blood Glucose, Cardiovascular and Kidney Functions of Rats, and on Kidney Cell Lines of the Proximal (LLC-PK1) and Distal Tubules (MDBK)

“…Cell culture systems provide a good model for the evaluation of cytotoxicity of various compounds. We, therefore, employed the extensively used in vitro cell culture techniques of the proximal (LLC-PK1) and distal tubule (MDBK) cells [5][6][7] to study FTE-induced renal effects. The technique mimics the in vivo state, as these cell lines maintain similar biochemical function of levels of marker enzymes exhibited by freshly isolated cells.…”

İnsektisit Endosülfana Maruz Kalan Hayvansal ve Böcek Kökenli Hücre Kültürlerindeki Proliferasyon ve LDH Fazlalığı

“…Cell lines used in this study were also used as model systems for testing of various xenobiotics, e.g. WBF344 for food and perfume additives 13 , RK13 for mycotoxins 14 , and the cell line Sf21 for testing of insect cell extracts 15 or novel insecticides 16 . Manji and Friesen 17 and Tseng et al 18 studied induction of apoptosis and Takahashi et al 19 cell cycle arrest induced by radiation in SF21 cell line.…”