Cytotoxic and photocytotoxic effect of Photofrin® on human laryngeal carcinoma (Hep2c) cell line

Firdous, S.; Mahmood, Rashid; Fakhar-e-Alam, M.; Zaidi, S. S. Z.; Suleman, R.; Ikram, Manzoor; Nawaz, M.

doi:10.1134/s1054660x11130020

Cited by 27 publications

(21 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ALA absor bance in RD cells is very low initially but after 22 h of incubation time there is an accidentally increase in ALA absorbance which shows behavior up till 47 h of time of incubation. It is an excellent agreement with previous reported results [32][33][34][35][36]. Our group has already investigated that two different selected con centration of ALA present almost same uptake behav ior.…”

Section: Resultssupporting

confidence: 92%

“…Atif et al [33] concluded that nei It was experimentally proved that 30-55 μg/ml, 100 to 250 μg/ml, and 350 to 400 μg/ml concentration of ALA, the viability for HeLa, Hep2c, HepG2, RD, and HEK293T cell lines becomes minimum of about 18 and 28%, respectively, as shown in Fig. 2 and dis cussed in our previous research papers [32][33][34][35][36]. Many researchers has worked with different photosensitizers in different cell line, e.g., Photofrin mediated PDT in gastric cancer cell line (MKN45) induces apoptosis within 60 min, and mitochondrial damage is likely as the first event of apoptosis.…”

Section: Resultsmentioning

confidence: 78%

“…It is investigated that very sharp absorbance peak at 400, 250, 30, and 55 μg/ml were observed for HEK293T, RD, HeLa, HepG2, and Hep2c cell line, the above mentioned drug dose for cell lines are called optimal dose. Our group explored the data for cytotoxicity and phototoxicity, PS uptake for different malignant cell lines in our previous work [32][33][34][35][36]. It is worth mentioning that no protocol avail able on PDT mediated aforementioned cell lines before our work.…”

Section: Resultsmentioning

confidence: 97%

“…Some other researcher founded that the immune response is initiated by the cellular components of osseous portion [30,31]. We have concluded in our recent publish data by using dif ferent cell lines that cell killing is light dose dependent associated with photosensitizer concentration [32][33][34][35][36]. Current study is to optimize PDT parameters like PS concentration, incubation time and light dose on five cancer cell lines and to study the cell viability.…”

Section: Introductionmentioning

confidence: 82%

“…The cell culture with 70-80% confluence was har vested using 0.20-0.25% trypsin. Similar technique was adopted in our previous published data [32][33][34][35][36].…”

Section: Cells and Culture Conditionsmentioning

confidence: 95%

See 4 more Smart Citations

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

et al. 2012

Self Cite

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is a kind of photochemo therapeutic treatment that exerts its effect mainly through the induction of cell death. Distinct types of cell death may be elicited by different PDT regimes. In this study, efforts are underway to optimize PDT protocols for improved efficacy and combination of all three PDT mechanisms involved in the different human carcinomas cell narcosis. Our in vitro cell cul ture experiments with 5 aminolevulanic acid (ALA) a clinically approved photiosensitizer (PS) and 635 nm laser light have yielded promising results, as follow: (1) (human cervical cancer (HeLa) cell line incubated, for 18 h, with 30 µg/ml of 5 ALA, treated with laser light dose of 50 J/cm 2 can produce 85% of cell killing (2) human larynx carcinoma (Hep2c) cell line incubated, for 7 h, with 55 µg/ml of 5 ALA, treated with laser light dose of 85 J/cm 2 can produce 75% of cell killing (3) human liver cancer (HepG2) cell line incubated, for 22-48 h, with 262 µg/ml of 5 ALA, treated with laser light dose of 120 J/cm 2 can produce 95% of cell killing (4) human muscle cancer (RD) cell line incubated, for 47 h, with 250 µg/ml of 5 ALA, treated with laser light dose of 80 J/cm 2 can produce 76% of cell killing (5) Human embryonic kidney (HEK293T) cell line incu bated, for 18 h, with 400 µg/ml of 5 ALA, treated with laser light dose of 40 J/cm 2 can produce 82% of cell killing confirming the efficacy of photodynamic therapy.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 82%

“…The cell culture with 70-80% confluence was har vested using 0.20-0.25% trypsin. Similar technique was adopted in our previous published data [32][33][34][35][36].…”

Section: Cells and Culture Conditionsmentioning

confidence: 95%

See 3 more Smart Citations

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

Synthesis, Characterization, and Application of BaTiO3 Nanoparticles for Anti-Cancer Activity

Fakhar-e-Alam¹,

Saddique²,

Hossain

et al. 2022

J Clust Sci

View full text Add to dashboard Cite

Barium titanate (BaTiO3) nanoparticles (BTNPs) have been considered as emerging materials in biomedical sector through last decades due to the excellent physicochemical properties such as dielectric and piezoelectric structures, biocompatibility, and nonlinear optical characteristics. In this study, BTNPs were synthesized via the co-precipitation method using barium carbonate and titanium dioxide by stirring for 5 h. Then, it was annealed at 850 °C for 5 h with five different concentrations: 0.2, 0.4, 0.6, 0.8, and 1 g/mL. The structural, morphological, and optical analyses were demonstrated by different characterization techniques such as X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), thermogravimetric analysis (TGA), Raman, and UV–visible spectroscopy. The perovskite phase of BTNPs, an intense peak at 31.6°, was observed at the lowest concentration (0.2 g/mL), and the average crystalline size was 1.42 nm based on XRD pattern. The results have been justified by SEM and EDX. TGA demonstrated the adequate thermal stability of this material. EDX analysis confirmed the composition of Ti, Ba, and O elements. Raman peaks at 305 cm−1 and 517 cm−1 confirmed the formation of BaTiO3. UV–visible spectra presented that its’ absorbance edge shifted into visible range at 404 nm. Application of BTNPs on breast cancer cell line (MCF-7) presented significant dispersion effect at 0.2, 0.4 and 0.6 g/mL of BaTiO3. A strong toxicity rate of BaTiO3 has been observed against the MCF-7 cell line. Maximum % of cell viability loss, $$\cong$$ ≅ 57% was recorded at 200 µg/mL of BTNPs, and minimum % of cell viability loss was observed as 19% at 50 µg/mL of BTNPs. The results presented that a higher concentration of BTPNs dosage was more effective in inhibition of breast cancer cells. Therefore, BTNPs can be recommended as a promising nanomaterial for anti-cancer drug discovery.

show abstract

Low energy light induced DNA photocleavage with a bis-ruthenated porphyrin

Swavey

Wilson

2015

Inorganica Chimica Acta

View full text Add to dashboard Cite

Cytotoxic and photocytotoxic effect of Photofrin® on human laryngeal carcinoma (Hep2c) cell line

Cited by 27 publications

References 61 publications

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

Synthesis, Characterization, and Application of BaTiO3 Nanoparticles for Anti-Cancer Activity

Low energy light induced DNA photocleavage with a bis-ruthenated porphyrin

Contact Info

Product

Resources

About