Cytotoxic and spectroscopic studies on binding of a new synthesized bipyridine ethyl dithiocarbamate Pt(II) nitrate complex to the milk carrier protein of BLG

Green synthesis of zinc oxide nanoparticles and their effect on the stability and activity of proteinase K

“…Due to the very low quantum yield of phenylalanine and tyrosine, normally, uorescence of tryptophan residue has been investigated in researches. 48,49 Binding of a ligand to a protein may directly affect the uorescence of a tryptophan residue by acting as a quencher or physically interacting with uorophore, thereby changing the polarity of its environment and/or its accessibility to solvents. Both direct and generalized effects may result either in the enhancement or quenching of uorescence and/or red or blue spectra shi.…”

β‐lactoglobulin–pectin Nanoparticle‐based Oral Drug Delivery System for Potential Treatment of Colon Cancer

“…To form stable nano‐complexes with pectin within this pH range where BLG is positively charged, we added an excess of negatively charged pectin, which leads to the formation of soluble, negatively charged nano‐complexes which remain stable due to their mutual electrostatic repulsion . It is notable that in our previous reports, we have investigated the binding of the Pt(II) complex with BLG and found that approximately 3 molecules of Pt(II) complex can bind to one BLG molecule . Then, the concentration of Pt(II) complex was selected 112 μ m for the present study.…”

“…Bovine β‐lactoglobulin (BLG, AB), low‐methoxyl pectin (LMP), and 3‐(4,5‐dimethyl‐2‐thiazolyl‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) were purchased from Sigma Company (St Louis, MO, USA). The Pt(II) complex (ethyl bipyridine dithiocarbamate Pt(II) nitrate) was synthesized in our laboratory as previously described . All other solvents and reagents used were of analytical grade.…”

“…Thus, because of the physicochemical properties of the protein, in particular its stability at low pH and in the presence of protease in the stomach, and also its ability for binding hydrophobic ligands, BLG should be suitable for the transport of drugs . Working on this assumption, we selected the newly designed anticancer complex, bipyridine ethyl dithiocarbamate Pt(II) nitrate (Figure ) , with BLG–pectin NPs for this study.…”

New palladium(ii) and platinum(ii) complexes with an ONS donor azo-thioether pincer ligand: synthesis, characterization, protein binding study and in vitro cytotoxicity

“…Since the structure of Bovine Serum Albumin (BSA) is homologous with HSA and owing to its easy accessibility and low cost, BSA is mostly used in biochemical and biophysical studies. [30][31][32][33] In this paper, we have synthesized Pd(II) and Pt(II) complexes (Scheme 1) having the general formula [Pd(L)Cl] (1) and [Pt(L)Cl] (2) with ONS donor azo-thioether ligand (HL) and characterized both of them by using various spectroscopic techniques like NMR, IR, Mass and UV-Vis, as well as single crystal X-ray diffraction study. Finally, the binding properties of these complexes toward calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) are investigated with the help of absorption and fluorescence spectroscopy.…”