Cytotoxicity and antimicrobial activities of green synthesized silver nanoparticles

Lokina, S.; Kaviyarasan, V.; Chinnasamy, Arulvasu; Narayanan, Vijaykrishnan

doi:10.1016/j.ejmech.2014.02.010

Cited by 124 publications

(74 citation statements)

References 33 publications

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“…Both BNPs derived from leaf and flower, respectively appeared to be good inhibitors of α-amylase at a dose as low as 0.0002 mg/ml. 53] were present in the phytochemical mixture with the synthesized Au-Ag BNPs. The significant biological activity displayed by O. basilicum derived Au-Ag BNPs compared to their various crude extracts and acarbose may be due to the increasing the surface area phenomenon (promoting electron transfer reaction from adjacent Ag atoms to Au atoms) and increased pharmacokinetics thereafter, thus, contributing to the enhanced inhibition of Gram-positive and Gram-negative bacterial cells, α-amylase and α-glucosidase enzymes.…”

Section: Inhibitory Effects Of Au-ag Bnps On α-Amylase and α-Glucosidmentioning

confidence: 99%

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Ramdas¹,

Mbatha²,

Gengan³

et al. 2015

Adv. Mater. Lett.

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This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles. The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa.

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Section: Inhibitory Effects Of Au-ag Bnps On α-Amylase and α-Glucosidmentioning

confidence: 99%

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Ramdas¹,

Mbatha²,

Gengan³

et al. 2015

Adv. Mater. Lett.

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“…The silver ions then combined with thiol, hydroxyl, and carboxyl group in the cell, deactivate several functions in the cell and damage the cells. Silver nanoparticles combined with respiratory enzyme, protease enzyme and interact with the sulfur and phosphorus of the DNAs of bacteria to cause suffocation, indigestion, inhibition of cell replication, respectively, and thus terminate the microbes (Lokina et al 2014;Sukumaran and Eldho 2012). The mechanism of antifungal potential has been suggested as the deactivation of sulfhydryl groups of the fungal cell wall, and onward formation of insoluble compounds followed by the disruption of membrane bound enzymes and lipids finally resulting in cell lysis (Ajitha et al 2015).…”

Section: Organisms Zone Of Inhibition (Mm)mentioning

confidence: 99%

Biosynthesis of silver nanoparticles using aqueous leaf extract of Thevetia peruviana Juss and its antimicrobial activities

et al. 2015

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Biosynthesizing of silver nanoparticles using microorganisms or various plant parts have proven more environmental friendly, cost-effective, energy saving and reproducible when compared to chemical and physical methods. This investigation demonstrated the plant-mediated synthesis of silver nanoparticles using the aqueous leaf extract of Thevetia peruviana. UV-Visible spectrophotometer was used to measure the surface plasmon resonance of the nanoparticles at 460 nm. Fourier Transform Infrared showed that the glycosidic -OH and carbonyl functional group present in extract were responsible for the reduction and stabilization of the silver nanoparticles. X ray diffraction, Scanning Electron Microscopy, Transmission Electron Microscopy and Selected Area Electron Diffraction analyses were used to confirm the nature, morphology and shape of the nanoparticles. The silver nanoparticles are spherical in shape with average size of 18.1 nm. The synthesized silver nanoparticles showed activity against fungal pathogens and bacteria. The zone of inhibition observed in the antimicrobial study ranged between 10 and 20 mm.

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“…To optimize the IC50 (the concentration at which 50% growth inhibition was observed is IC50) of the AuNPs 24 h. It was observed that treatment with the synthesized AuNPs showed significantly decreased cell viability as compared to control. 40-50% cell viability was found in the concentration of 80 µg and 100 µg at 24 h of incubation [22][23][24][25][26][27][28]. Anticancer property of AuNPs was evaluated in DL cell cells by using primarily trypan blue exclusion assay, which shows the growth inhibitory activity of DL cells.…”

Section: Discussionmentioning

confidence: 99%

“…Gold nanoparticle was synthesized as per previously described method [22,23]. In brief, 100 g of freshly harvested leaf of tulsi (Osmium sanctum) was taken and washed gently with distilled water.…”

Section: Synthesis Of Gold Nanoparticles By Tulsi (Osmium Sanctum)mentioning

confidence: 99%

Biologically Synthesized Gold Nanoparticles using Ocimum sanctum (Tulsi Leaf Extract) Induced Anti-Tumor Response in a T Cell Daltons Lymphoma

Gautam¹,

Kumar²,

Ms³

et al. 2017

J cell Sci Ther

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The characterizations of green synthesized gold nanoparticles (AuNPs) prepared using Ocimum sanctum leaf extract were done by UV-Spectrophotometer at 500-540 nm. The XRD data obtained were found similar to gold JCPDS File No-04-0784. SEM and TEM analysis of AuNPs revealed spherical shape and size of 12-20 nm. Further FT-IR data indicated the various biomolecules present in Ocimum sanctum leaf extract provides stability to gold nanoparticles synthesis. The AuNPs were studied for their anti-cancer activity on Dalton's lymphoma (DL) cells and the results obtained with IC50 value of <50 ng/ml performed by MTT assay. Further, to confirm anti-tumor potential and the mode of action of the synthesized AuNPs, cell viability assay, nuclear morphology, DNA fragmentation assay, mitochondrial membrane potential (ΔΨm) analysis, and cell cycle analysis were done using DL cells. DL cells treated with the AuNPs showed reduced cell viability, altered nuclear morphology, typical apoptotic DNA ladder formation and apoptosis. From the above finding it can be concluded that the AuNPs have potential to decrease the proliferation of tumor cells and enhanced the production of ROS. Gold nanoparticles used in cancer detection and diagnosis/treatment are mainly in preclinical stages of cancer development.

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Cytotoxicity and antimicrobial activities of green synthesized silver nanoparticles

Cited by 124 publications

References 33 publications

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Biosynthesis of silver nanoparticles using aqueous leaf extract of Thevetia peruviana Juss and its antimicrobial activities

Biologically Synthesized Gold Nanoparticles using Ocimum sanctum (Tulsi Leaf Extract) Induced Anti-Tumor Response in a T Cell Daltons Lymphoma

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