Cytotoxicity and Apoptosis Induction of Bovine Alpha-lactalbumin-oleic Acid Complex in Human Breast Cancer Cells

Improved antitumor activity and IgE/IgG–binding ability of α‐Lactalbumin/β‐lactoglobulin induced by ultrasonication prior to binding with oleic acid

“…Figure 1a,b shows that α‐La or β‐Lg alone did not decrease the number of cancer cells, while the N‐La‐OA or N‐Lg‐OA caused a dramatic loss of cell viability but less than that of U‐La‐OA or U‐Lg‐OA. This result suggested that the binding of OA to α‐La or β‐Lg produce the complex α‐La/β‐Lg‐OA, which is a tumoricidal complex (Fang et al., 2015; Zhong et al., 2015). The maximum effect was observed with 4 mg/ml U‐La‐OA, which inhibited the proliferation of MCF‐7 and Caco‐2 cell lines by 38.2% and 33.7%, respectively (Figure 1a).…”