Cytotoxicity and chromosome aberrations in normal human oral keratinocytes induced by chemical carcinogens: Comparison of inter-individual variations

Tsutsui, Takeki; Kawamoto, Yukihiro; Suzuki, Nobuko; Gladen, Beth C.; Barrett, J. Carl

doi:10.1016/0887-2333(91)90013-4

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…1987Sat0 et al, 1989Jachimczak and Scott and Roberts, 1987Suzuki and Tsutsui. 1989Skotarczak, 1978Tsutsui et al, 1984cTsutsui et al. 1991Gebhart et al.…”

Section: Human Cellsmentioning

confidence: 99%

Genetic toxicity of fluoride

Zeiger

Shelby

Witt

1993

Environ and Mol Mutagen

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F- is not mutagenic in standard bacterial systems, but produces chromosome aberrations and gene mutations in cultured mammalian cells. Although there is disagreement in the literature concerning the ability of F- to induce chromosome aberrations in cultured human and rodent cells, the weight of the evidence leads to the conclusion that F- exposure results in increased chromosome aberrations in these test systems. NaF induced primarily chromatid gaps and chromatid breaks, indicating that the rodent cells are responsive in the G2 stage of the cell cycle. In contrast, studies with synchronized human cells indicated that the S phase was the most sensitive. If F- does have a cell cycle-specific effect, it could be expected that differences in the cell treatment and harvest protocols could lead to conflicting results for the induction of chromosome aberrations. Gene mutations were produced in cultured rodent and human cells in the majority of the studies. Unfortunately, a number of the in vitro and in vivo cytogenetic studies are of questionable utility because of the protocols used, the quality of the responses reported, or the interpretations of the data. The conflicting results in the in vivo cytogenetic studies are difficult to reconcile. There are reports of increased chromosome aberrations in rat bone marrow and testes, but other studies, using similar protocols and dose ranges, have reported no induced chromosome damage. Although some of the studies were performed at toxic levels of F-, other studies, including those that showed positive results, were at F- concentrations (1-5 ppm) equivalent to human exposure levels. In the majority of studies that were reported to be positive, there were high background frequencies, or the investigators reported categories of nuclear or chromosome damage that are difficult to interpret. Interestingly, many of the positive results were obtained when anaphase cells were scored, whereas similar treatment protocols in other laboratories yielded negative results when metaphase cells were the only cell type examined. It is difficult, without additional data, to determine the reasons for finding chromosome breaks in anaphase, but not metaphase, cells. Other reports have presented insufficient information to allow adequate evaluations. Therefore, at this time, the question of whether F- produces chromosome damage in vivo should be considered unresolved.

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“…1987Sat0 et al, 1989Jachimczak and Scott and Roberts, 1987Suzuki and Tsutsui. 1989Skotarczak, 1978Tsutsui et al, 1984cTsutsui et al. 1991Gebhart et al.…”

Section: Human Cellsmentioning

confidence: 99%

Genetic toxicity of fluoride

Zeiger

Shelby

Witt

1993

Environ and Mol Mutagen

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“…25,26 Briefl y, gingival tissues around the third molar of the mandible were obtained from a 24-year-old woman in apparently good health at the Nippon Dental University Hospital in Tokyo under guidelines approved by the Committee of Ethics, Nippon Dental University of Life Dentistry at Tokyo. Histopathological examinations provided no evidence of benign or malignant growth in these tissues.…”

Section: Methodsmentioning

confidence: 99%

Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells

et al. 2009

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Most in vitro studies of oral carcinogenesis in human cells are carried out with oral keratinocytes immortalized by human papillomavirus type 16 DNA. However, because various etiological factors for oral cancer are known, it is important to establish new human keratinocyte cell lines useful for studying the mechanism of oral carcinogenesis. Normal human gingival keratinocytes in secondary cultures grown in serum-free medium were either transfected with origin (-) SV40 DNA or sequentially transfected with origin (-) SV40 DNA and human c-fos. The transfected cells were continually passaged and analyzed for cytological and cytogenetic characterizations. Four immortal cell lines were grown for over 1100 days in culture and maintained a vigorous growth for over 250 population doublings. They expressed SV40 T antigen, cytokeratins 8 and 18, and E-cadherin, and overexpressed the c-Fos protein. The immortal cell lines had telomerase activity but lacked transformed phenotypes on soft agar or in nude mice. Each cell line had nonrandom chromosomal abnormalities and minisatellite alterations. One of the immortal cell lines, NDUSD-1, retained the capability to deposit calcium, which was also demonstrated in normal human gingival keratinocytes by alizarin red staining, indicating the possibility that NDUSD-1 cells may retain some natural characteristics of normal gingival keratinocytes. Because the oral ectoderm plays an important role in tooth development, these immortal cell lines may be useful in various experimental models for investigations of oral biology and oral carcinogenesis.

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Fluoride [MAK Value Documentation in German language, 2006]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 40. Lieferung, Ausgabe 2006 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Aufnahme, Verteilung, Ausscheidung Metabolismus Erfahrungen beim Menschen Einmalige Exposition Wiederholte Exposition Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Akute Toxizität Subakute, subchronische und chronische Toxizität Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Kanzerogenität Bewertung

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Cytotoxicity and chromosome aberrations in normal human oral keratinocytes induced by chemical carcinogens: Comparison of inter-individual variations

Cited by 9 publications

References 17 publications

Genetic toxicity of fluoride

Genetic toxicity of fluoride

Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells

Fluoride [MAK Value Documentation in German language, 2006]

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