Cytotoxicity evaluation of nanoclays in human epithelial cell line A549 using high content screening and real-time impedance analysis

Verma, Navin Kumar; Moore, Edward W.; Blau, Werner J.; Volkov, Yuri; Babu, P. Ramesh

doi:10.1007/s11051-012-1137-5

Cited by 70 publications

(49 citation statements)

References 20 publications

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“…Some (Byrne and Baugh 2008, Nel et al 2006) have expressed concern that nanoparticles can be dangerous and that platelet and tubular Al nanoclays are cytotoxic (e.g., Lordan and Higginbotham 2012, Lordan et al 2011, Verma et al 2012, Wallace et al 1985). However there is little correlation between Al nanoparticle toxicity in vitro and that observed in vivo, partly because the physical properties of the primary Al nanoparticles differ from the larger aggregates (Al nanoclusters) (Murdock et al 2008) to which people are exposed.…”

Section: Synthesis and Conclusionmentioning

confidence: 99%

Systematic review of potential health risks posed by pharmaceutical, occupational and consumer exposures to metallic and nanoscale aluminum, aluminum oxides, aluminum hydroxide and its soluble salts

Willhite¹,

Karyakina²,

Yokel³

et al. 2014

Critical Reviews in Toxicology

339

201

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Aluminum (Al) is a ubiquitous substance encountered both naturally (as the third most abundant element) and intentionally (used in water, foods, pharmaceuticals, and vaccines); it is also present in ambient and occupational airborne particulates. Existing data underscore the importance of Al physical and chemical forms in relation to its uptake, accumulation, and systemic bioavailability.Address for correspondence: Calvin C. Willhite, Risk Sciences International, 55 Metcalfe Street, Suite 700, Ottawa, ON, Canada. calvinwillhite@hotmail.com. Declaration of interestPartial funding for this work was provided by a contract to review the recent scientific literature on health effects of aluminum between the International Aluminium Institute (IAI, www.world-aluminium.org), the Aluminium Reach Consortium (ARC, www.aluminium-reach-consortium.eu), and Risk Sciences International (RSI, www.risksciences.com), a Canadian company established in 2006 in partnership with the University of Ottawa. Additional financial support was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) to D. Krewski, who holds the NSERC Chair in Risk Science at the University of Ottawa. C.C. Willhite, N.A. Karyakina, F. Momoli, and N. Yenugadhati were compensated by RSI for their contributions to the review. I. Arnold, T. Wisniewski and R. Yokel received no compensation from RSI for their contributions to this work. The authors, whose affiliations are shown on the title page, had sole responsibility for preparation of this paper, including determining the strategy for reviewing the scientific literature summarized in this article, synthesizing the findings, and drawing conclusions. Scientists associated with IAI/ARC were given the opportunity to review and offer technical comments on the paper, prior to submission to Critical Reviews in Toxicology. I. Arnold serves as a consultant to the International Aluminium Institute. None of the authors have appeared before regulatory agencies on behalf of the sponsors, or appeared as experts in legal proceedings concerning matters reviewed in this paper. The scientific opinions and conclusions expressed in the paper are exclusively those of the authors, and are independent of the sources of financial support. HHS Public Access Author Manuscript Author ManuscriptAuthor Manuscript Author ManuscriptThe present review represents a systematic examination of the peer-reviewed literature on the adverse health effects of Al materials published since a previous critical evaluation compiled by Krewski et al. (2007). The scientific literature on the adverse health effects of Al is extensive. Health risk assessments for Al must take into account individual co-factors (e.g., age, renal function, diet, gastric pH). Conclusions from the current review point to the need for refinement of the PTWI, reduction of Al contamination in PN solutions, justification for routine addition of Al to vaccines, and harmonization of OELs for Al substances.

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Section: Synthesis and Conclusionmentioning

confidence: 99%

Systematic review of potential health risks posed by pharmaceutical, occupational and consumer exposures to metallic and nanoscale aluminum, aluminum oxides, aluminum hydroxide and its soluble salts

Willhite¹,

Karyakina²,

Yokel³

et al. 2014

Critical Reviews in Toxicology

339

201

View full text Add to dashboard Cite

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“…Verma et al (2012) compared the toxicity of both structures in the lung epithelial cells A549 and showed that the platelet-structured nanoclays were more cytotoxic than the tubular types. It is important to note that the structure was not the only difference among them because the nature of the clay mineral also differed.…”

Section: Basal Cytotoxicity Assays and Morphological Studiesmentioning

confidence: 99%

Toxicological evaluation of clay minerals and derived nanocomposites: A review

Maisanaba

Pichardo

Puerto

et al. 2015

Environmental Research

194

125

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“…Previous studies have shown that mammalian cell cytotoxicity of PEIs depend upon the polymer molecular weight and exposure time [35]. To obtain a better insight into possible LPEI/BPEI-induced mammalian toxicity, we used High Content Analysis (HCA), a high throughput quantitative data acquisition method for determining changes in cellular behaviour and morphotypes [60][61][62]. For this study, human dermal fibroblasts (hDFs) cells were exposed to various concentrations of the polymers for 24 h, fluorescently stained with nuclear and cytoplasmic markers and analysed by using an automated INCell Analyzer 2200.…”

Section: In Vitro Evaluation Of Membrane Selectivity Of Lpei and Bpeimentioning

confidence: 99%

Insight into membrane selectivity of linear and branched polyethylenimines and their potential as biocides for advanced wound dressings

et al. 2016

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Cytotoxicity evaluation of nanoclays in human epithelial cell line A549 using high content screening and real-time impedance analysis

Cited by 70 publications

References 20 publications

Systematic review of potential health risks posed by pharmaceutical, occupational and consumer exposures to metallic and nanoscale aluminum, aluminum oxides, aluminum hydroxide and its soluble salts

Systematic review of potential health risks posed by pharmaceutical, occupational and consumer exposures to metallic and nanoscale aluminum, aluminum oxides, aluminum hydroxide and its soluble salts

Toxicological evaluation of clay minerals and derived nanocomposites: A review

Insight into membrane selectivity of linear and branched polyethylenimines and their potential as biocides for advanced wound dressings

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