Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts

Samyuktha, Voruganti; Ravikumar, Pabbati; Bolla, Nagesh; Ranganathan, K; Jayaprakash, Thumu; Vemuri, Sayesh

doi:10.4103/0972-0707.139844

Cited by 27 publications

(28 citation statements)

References 13 publications

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“…Endosequence and Biodentine; Samyuktha et al . ). Therefore, the 1 : 8 (lowest non‐cytotoxic dilution) was the one chosen to assess the biological activity of these materials.…”

Section: Discussionmentioning

confidence: 97%

An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine

Rodrigues¹,

Gomes-Cornélio²,

Soares-Costa³

et al. 2017

Int Endodontic J

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Aim To evaluate the effect of MTA and Biodentine on viability, osteogenic differentiation and BMP-2 expression in osteogenic cells. Methodology Saos-2 cells were used as a model of osteoblastic cells. Overexpression of BMP-2 was induced by transfection of a CMV-driven plasmid construct including the human BMP-2 coding sequence, and stably transfected cells were selected. Cell viability was assessed by the mitochondrial dehydrogenase enzymatic (MTT) assay. The bioactivity of the materials was evaluated by the alkaline phosphatase (ALP) assay and detection of calcium deposits with alizarin red staining (ARS). The gene expression of BMP-2 and ALP was quantified with real-time PCR. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post-test (a = 0.05). Results Viability tests revealed that MTA and Biodentine were not cytotoxic at the higher dilution (1 : 8) to BMP-2-transfected cells. MTA and Biodentine exhibited the highest ALP activity when compared to the Saos-BMP-2-unexposed control group (P < 0.05). Cell exposure to Biodentine and MTA had a significant stimulatory effect on the formation of mineralized nodules (P < 0.05). The highest increase in BMP-2 gene expression was observed after 3 days of BMP-2-transfected cells exposure to MTA and Biodentine in non-osteogenic medium in relation to Saos-BMP-2-unexposed control cells (P < 0.05). Exposure of cells to MTA in osteogenic medium for 1 day increased ALP gene expression by approximately 1.3-fold in relation to Saos-BMP-2-unexposed control cells (P < 0.05). Conclusions Both MTA and Biodentine showed biocompatibility and bioactivity in Saos-BMP-2 overexpressing cells. Biodentine had a significantly greater effect on mineralization than MTA. Both MTA and Biodentine enhanced BMP-2 mRNA expression in the transfected system. Both MTA and Biodentine are suitable materials to improve osteoblastic cell mineralization.

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“…Endosequence and Biodentine; Samyuktha et al . ). Therefore, the 1 : 8 (lowest non‐cytotoxic dilution) was the one chosen to assess the biological activity of these materials.…”

Section: Discussionmentioning

confidence: 97%

An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine

Rodrigues¹,

Gomes-Cornélio²,

Soares-Costa³

et al. 2017

Int Endodontic J

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“…Even if at the first 24 hours all the materials showed increased cell viability, at 48 hours, there is a slight decrease in cell viability. MTA showed statistically significant increase in the cell viability when compared to Endosequence Root Repair Material and Biodentine [20]. We can conclude that MTA shows better biocompatibility and may be indicated for sealing of communication between periodontal and endodontic space.…”

Section: Some Characteristics Of Bioceramics Used In Endodonticsmentioning

confidence: 99%

The Use of Bioceramics in Endodontics - Literature Review

Jitaru¹,

Hodisan²,

Timis³

et al. 2016

Medicine and Pharmacy Reports

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Background and aimBioceramics are ceramic compounds obtained both in situ and in vivo, by various chemical processes. Bioceramics exhibit excellent biocompatibility due to their similarity with biological materials, like hydroxyapatite. Bioceramics and multi-substituted hydroxyapatite or similar compounds have the ability to induce a regenerative response in the organism.The aim of this paper is to make a literature review on the main bioceramic materials currently used in endodontics and on their specific characteristics.MethodsWe conducted a search in the international databases (PubMed), to identify publications in the last 10 years, using the following key words: “bioceramics endodontics”, “bioceramic endodontic cement”, “bioceramic sealer” and “direct pulp capping bioceramic”.ResultsCommonly used endodontic sealers (e.g., containing zinc oxide, calcium hydroxide and a resin) have a long tradition in scientific research and clinical use in endodontics. For specific cases, like root resorptions, perforations, apexification, and retrograde fillings, new biocompatible materials were developed in order to improve the clinical outcome: ProRooT MTA (Dentsply Company, Germany); Biodentine (Septodont, France); Endosequence BC sealer (Brassler, SUA); Bioaggregate (IBC, Canada); Generex A (Dentsply Tulsa Dental Specialties, USA).ConclusionThe studies are generally in favor of bioceramic materials even if there are not many products available on the market for endodontic use. As more products are launched and more research is performed regarding these materials, we will provide more reliable data on clinical outcome.

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“…[36] Our results show that MTA-derived materials could enhance the cell viability of 3T3 cells in vitro. Recent studies showed that the cytotoxic effects imposed by Ortho MTA, [26,27] Retro MTA, [37] BioAggregate, [18,28,29] Biodentine, [26,28,38,39] MTA Plus [40] and MTA Angelus exhibited negligible cytotoxicity. [41,42] While the extracts of BioAggregate promoted cell viability, Biodentine showed a reduced number of cells.…”

Section: Microscopy Resultsmentioning

confidence: 99%

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

Varol

Bahar

Karsli

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the effects of calcium silicate-based products on cytotoxicity in the 3T3 fibroblast and gelatinolytic activity of matrix metalloproteinases (MMPs). 3T3 fibroblasts were incubated directly with Ortho Mineral trioxide aggregate (MTA), BioAggregate, Biodentine, MTA Plus, MTA Angelus and MTA Cerkamed for 24 hours and seven days. The cytotoxicity was determined using an MTT assay. Supernatants were collected to determine MMP-2 and MMP-9. Data were analysed using IBM SPSS 22. Seventh day extracts of Ortho MTA and Biodentine showed reduced cell viability. Specific characterization of MMPs in cell culture demonstrated that MMP-2 (62 kPa) in the cell culture supernatants by gelatin zymography showed induced expression in four out of seven groups by 3T3 cells. No MMP-9 expression was observed. The cytotoxicity of materials revealed a significant difference in cell viability between the groups on the first and seventh days. The results of this study revealed minor cytotoxic effects for Ortho MTA and Biodentine. This study suggests that endodontic sealers induced production of MMP-2. MMP-9 might be expressed in small amounts when compared with MMP-2.

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Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts

Cited by 27 publications

References 13 publications

An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine

An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine

The Use of Bioceramics in Endodontics - Literature Review

Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

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