Cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and response of rat pulp to MTA and BMP-2

Ko, Hyunjung; Yang, Wenjun; Park, Kyungtae; Kim, Miri

doi:10.1016/j.tripleo.2010.01.030

Cited by 27 publications

(25 citation statements)

References 23 publications

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“…To determine cytotoxicity, various methods have been recommended, including MTT-based colorimetric assay. The method is a rapid and simple test which identifies only living cells what allows measuring cell cytotoxicity and proliferation [8,9]. Since the therapeutic effects of direct pulp capping materials are related to their alkalizing abilities, it is important to define the pH values of commercial products.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials — an in vitro study

Łuczaj-Cepowicz¹,

Marczuk-Kolada²,

Pawińska³

et al. 2017

Folia Histochem Cytobiol.

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Introduction. Various materials are used in direct dental pulp capping method. Their biocompatibility and alkalizing abilities are of primary importance affecting therapeutic effects. The aim of this study was to evaluate and compare the cytotoxicity of various pulp-capping materials on human gingival fibroblasts and investigate the pH changes induced by these materials. Material and methods. Human gingival fibroblasts were cultured with nine direct pulp materials using culture plate inserts. The cytotoxic effects were recorded by using an MTT-based colorimetric assay after 3 and 24 h. In the second part of the experiment, the materials were inserted in dialysis tubes and transferred into plastic vials containing deionized water. The changes of the medium pH were measured after 3 and 24 h. Results. We showed differences in cell viability of gingival fibroblasts after varied time of exposition for the tested materials. Cell viability after 24 h increased for Dycal, Biopulp, and Calcipro, and decreased for Calcipulpe, Angelus, Angelus White, and ProRoot Regular. Cell viability for ProRoot and Life did not change. Non-setting calcium hydroxide preparations followed by the MTA group and setting calcium hydroxide materials produced the highest pH. All the tested materials significantly increased pH (p < 0.0001) at 24 h. Conclusions. Currently used pulp capping materials varied in their cytotoxicity relative to human gingival fibroblasts and their alkalizing capacities. Since most likely pH does not affect the viability of cultured cells, further investigations are required to determine physicochemical properties of these materials and the biological activity of the dental pulp.

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Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials — an in vitro study

Łuczaj-Cepowicz¹,

Marczuk-Kolada²,

Pawińska³

et al. 2017

Folia Histochem Cytobiol.

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show abstract

“…Moreover, BMP-2 promotes osteoblast maturation by increasing the expression of certain transcription factors and osteoblast marker genes 9. In our previous study, we showed that BMP-2 reduces the initial cytotoxicity of freshly mixed MTA in vitro 10. Several BMPs have been tested experimentally, with the aim of inducing reparative dentinogenesis in dental pulp 11…”

Section: Introductionmentioning

confidence: 99%

The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells

Jeong

Yang

et al. 2014

Restor Dent Endod

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ObjectivesThe effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.Materials and MethodsMC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer).ResultsMineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).ConclusionsThese results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

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“…However, some studies have suggested that freshly mixed MTA exhibits some initial cytotoxicity, although its cytotoxicity is less than that reported for conventional materials such as calcium hydroxide (3). We previously revealed that MTA induces prostaglandin E 2 and the inflammatory markers cyclooxygenase-2 and inducible nitric oxide synthase via nuclear factor kappa B signaling pathway (4).…”

mentioning

confidence: 89%

Effect of N-acetylcysteine on Rat Dental Pulp Cells Cultured on Mineral Trioxide Aggregate

Minamikawa

Yamada

Deyama

et al. 2011

Journal of Endodontics

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Cytotoxicity of mineral trioxide aggregate (MTA) and bone morphogenetic protein 2 (BMP-2) and response of rat pulp to MTA and BMP-2

Cited by 27 publications

References 23 publications

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials — an in vitro study

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials — an in vitro study

The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells

Effect of N-acetylcysteine on Rat Dental Pulp Cells Cultured on Mineral Trioxide Aggregate

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