Cytotoxicity of modern dentin adhesives—in vitro testing on gingival fibroblasts

Abstract

The present investigation was designed to test cellular toxicity of modern dentin adhesives. With the use of the products Ariston Liner, Etch & Prime 3.0, Optibond Solo, Prime & Bond NT, Scotchbond 1, and Syntac Sprint, test specimens were prepared according to the manufacturers' instructions and transferred into a culture medium. Eluates were obtained and pipetted onto fibroblast cultures, incubated, and subsequently stained. The respective cell densities and the numbers of normal, altered, and dead cells were determined and compared with control cell cultures. Statistical analysis of the data showed that all materials caused cytotoxic effects. Scotchbond 1 displayed the highest number of dead cells. The difference was statistically significant compared to Etch" 3.0, Optibond Solo, Prime&Bond NT, and the control. The lowest cell density was found for Scotchbond 1 and Ariston Liner. The difference was also statistically significant in comparison with Etch" 3.0, Optibond Solo, Prime&Bond NT, and the control. To conclude, all tested dentin adhesives caused cytotoxic reactions. Taking the limitations of an in vitro experiment into consideration, Prime&Bond NT, Optibond Solo, and Etch" 3.0 appear to be the most recommendable products, and Scotchbond 1 and Ariston Liner the least.

Synthesis and characterization of triethylene glycol dimethacrylate nanocapsules used in a self-healing bonding resin

“…52,53 Previous studies also demonstrated that modern dentine adhesives, including Prime & Bond NT, exhibit cellular toxicity. 54,55 In the current study, the result of the MTT assay was consistent with those of previous studies. However, the addition of TEGDMA nanocapsules to this commercial adhesive did not affect cellular toxicity, indicating that TEGDMA nanocapsules met the biocompatibility requirements in clinical practice.…”

In VitroComparison of the Effects of Dental Filling Materials on Mouse Fibroblasts

“…It is known that, these restorative materials contain a great variety of monomers and additives (18). Some of these components, owing to the complex chemical composition and incomplete monomer-polymer conversion, leach out from resin-based restorative materials into the oral environment (55,57). Such leaching may cause adverse effects (11).…”

Biocompatibility of Dental Adhesives

“…bonding agent that contained "is-GM" was less cytotoxic than the other bonding agents that contained "is-GM" + UDM" + HEM" and "is-GM" + HEM" [ 0]. In addition, it was demonstrated that a combination of "is-GM" and HEM" was less cytotoxic than a combination of the following three substances: "is-GM", UDM", and HEM" [12]. HEM" is the most frequent hydrophilic monomer present in adhesive systems.…”

“…The determinant factor of the death was the monomer concentration, given that low concentrations of "is-GM" induced apoptosis, whereas high concentrations induced death by necrosis or late apoptosis. "is-GM" dissolves easily and may cause irritation or a lichenoid reaction of the oral mucosa in close and prolonged contact with gingival tissues, especially after cervical illing procedures [12,66]. TEGDM" is a copolymer used in major recent biomaterials with resin phase as a diluent.…”