Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

El‐Rahman, A. A. Abd; El-Shafei, Sally; Иванова, Е. В.; Фаттахова, А. Н.; Pankova, Anna Victorovna; El-Shafei, Mohamed Abd El-Aziz; El-Morsi, El-Morsi Abu El-Fotouh; Алимова, Ф. К.

doi:10.7314/apjcp.2014.15.17.7229

Cited by 20 publications

(14 citation statements)

References 27 publications

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“…PDF1 . 2 , AOC3 and ERF family, including ERF2 are induced resistance response to pathogen infection is activated synergistically by ethylene and jasmonic acid (JA) [23]. …”

Section: Discussionmentioning

confidence: 99%

“…Trichoderma spp. have the ability to produce a broad diversity of important secondary metabolites, especially those that play a role in bio-control mechanisms, such as the following secondary metabolites: pyrones, koninginins, trichothecenes, heptelidic acid, viridins, harzialactones, derivatives, ergosterol derivatives, peptaibols, anthraquinones, azaphilones, trichodermamides, butenolides, daucanes, acoranes, isocyano metabolites, butenolides, diketopiperazines, viridiofungins, trichodenones, cyclopentenone derivatives, cyclonerodiol derivatives, statins, setin-like metabolites, bisorbicillinoids, nitrogen heterocyclic compounds and others [22, 23]. …”

Section: Introductionmentioning

confidence: 99%

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Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

et al. 2017

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Plants have the ability to resist pathogen attack after infection or treatment with biotic and abiotic elicitors. In oilseed rape plant Brassica napus AACC and in the artificially synthesized Raphanus alboglabra RRCC, the root-colonizing Trichoderma harzianum TH12 fungus triggers induced systemic resistance (ISR), and its culture filtrate (CF) triggers a systemic acquired resistance (SAR) response against infection by the Sclerotinia sclerotiorum. Salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) are plant hormone signals that play important roles in the regulation of ISR and SAR. In this study, at six different time points (1, 2, 4, 6, 8 and 10 days post-infection [dpi]), six resistance genes were used as markers of signaling pathways: JA/ET signaling used AOC3, PDF1.2 and ERF2 genes, while PR-1, TGA5 and TGA6 genes were used as markers of SA signaling. The results of quantitative real-time polymerase chain reaction (qRT-PCR) showed that AOC3, PDF1.2 and ERF2 expression levels in infected leaves of AACC and RRCC increase at 1 and 2 dpi with S. sclerotiorum or inoculation with TH12. PR-1, TGA5 and TGA6 expression levels increased at 8 and 10 dpi in infected leaves. PR-1, TGA5 and TGA6 expression levels increased early in plants treated with CF in both of the healthy genotypes. Furthermore, induction of SA- and JA/ET-dependent defense decreased disease symptoms in infected leaves at different times. The results suggest that the RRCC genotype exhibits resistance to disease and that the ability of TH12 and its CF to induce systemic resistance in susceptible and resistant oilseed rape genotypes exists. In addition, the results indicate for the first time that in RRCC the SA signaling pathway is involved in resistance to necrotrophic pathogens.

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“…PDF1 . 2 , AOC3 and ERF family, including ERF2 are induced resistance response to pathogen infection is activated synergistically by ethylene and jasmonic acid (JA) [23]. …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

et al. 2017

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“…Although there are many newly emerged screening and therapeutic measures, the morbidity and mortality are still not satisfactorily controlled, posing a great challenge to public health. 1 Annually, it is estimated that ~1.3 million new BC cases are diagnosed worldwide. 2 …”

Section: Introductionmentioning

confidence: 99%

A dried blood spot mass spectrometry metabolomic approach for rapid breast cancer detection

Wang¹,

Sun²,

Cao³

et al. 2016

OTT

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ObjectiveBreast cancer (BC) is still a lethal threat to women worldwide. An accurate screening and diagnosis strategy performed in an easy-to-operate manner is highly warranted in clinical perspective. Besides the routinely focused protein markers, blood is full of small molecular metabolites with diverse structures and properties. This study aimed to screen metabolite markers with BC diagnosis potentials.MethodsA dried blood spot-based direct infusion mass spectrometry (MS) metabolomic analysis was conducted for BC and non-BC differentiation. The targeted analytes included 23 amino acids and 26 acylcarnitines.ResultsMultivariate analysis screened out 21 BC-related metabolites in the blood. Regression analysis generated a diagnosis model consisting of parameters Pip, Asn, Pro, C14:1/C16, Phe/Tyr, and Gly/Ala. Tested with another set of BC and non-BC samples, this model showed a sensitivity of 92.2% and a specificity of 84.4%. Compared to the routinely used protein markers, this model exhibited distinct advantage with its higher sensitivity.ConclusionBlood metabolites screening is a more plausible approach for BC detection. Furthermore, this direct MS analysis could be finished within few minutes, which means that its throughput is higher than the currently used imaging techniques.

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“…Several studies have reported that phytochemicals (including secondary metabolites from plants) are potential sources of agents possessing anticancer activity [18][19][20]. (+)-Usnic acid (dibenzofuran derivative) is a well-known secondary metabolite found in lichens.…”

Section: Discussionmentioning

confidence: 99%

(+)-Usnic Acid Induces ROS-dependent Apoptosis via Inhibition of Mitochondria Respiratory Chain Complexes and Nrf2 Expression in Lung Squamous Cell Carcinoma

Huang

et al. 2020

IJMS

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Lung squamous cell carcinoma (LUSC) has a poor prognosis, in part due to poor therapeutic response and limited therapeutic alternatives. Lichens are symbiotic organisms, producing a variety of substances with multiple biological activities. (+)-Usnic acid, an important biologically active metabolite of lichens, has been shown to have high anti-cancer activity at low doses. However, there have been no reports regarding the effect of (+)-usnic acid on LUSC cells. This study found that (+)-usnic acid reduced viability and induced apoptosis in LUSC cells by reactive oxygen species (ROS) accumulation. (+)-Usnic acid induced mitochondria-derived ROS production via inhibition of complex I and complex III of the mitochondrial respiratory chain (MRC). Interestingly, the elimination of mitochondrial ROS by Mito-TEMPOL only partially reversed the effect of (+)-usnic acid on cellular ROS production. Further study showed that (+)-usnic acid also induced ROS production via reducing Nrf2 stability through disruption of the PI3K/Akt pathway. The in vitro and in vivo xenograft studies showed that combined treatment of (+)-usnic acid and paclitaxel synergistically suppressed LUSC cells. In conclusion, this study indicates that (+)-usnic acid induces apoptosis of LUSC cells through ROS accumulation, probably via disrupting the mitochondrial respiratory chain (MRC) and the PI3K/Akt/Nrf2 pathway. Therefore, although clinical use of (+)-usnic acid will be limited due to toxicity issues, derivatives thereof may turn out as promising anticancer candidates for adjuvant treatment of LUSC.

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Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

Cited by 20 publications

References 27 publications

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

A dried blood spot mass spectrometry metabolomic approach for rapid breast cancer detection

(+)-Usnic Acid Induces ROS-dependent Apoptosis via Inhibition of Mitochondria Respiratory Chain Complexes and Nrf2 Expression in Lung Squamous Cell Carcinoma

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