Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles

Guan, Rongfa; Kang, Tian-Shu; Lü, Fei; Zhang, Zhiguo; Shen, Haitao; Liu, Mingqi

doi:10.1186/1556-276x-7-602

Cited by 170 publications

(97 citation statements)

References 28 publications

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“…When HEK 293 cell line was treated with 0-100 μg/mL zinc oxide nanoparticles for 24 h, it was observed that there was a reduction in cell viability. Results also demonstrated DNA damage, oxidative stress and mitochondrial damage [218]. Similarly, when human bronchial epithelial cells were treated with zinc oxide at 100 μg/mL, the observed effects include the release of LDH, decrease in cell viability and oxidative stress [219].…”

Section: Mechanistic Studiesmentioning

confidence: 92%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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Section: Mechanistic Studiesmentioning

confidence: 92%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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“…12 Other, more recent reports demonstrate the use of these cells for investigating the potential toxicity of nanoparticles. [13][14][15][16] Andelman et al examined the effects of exposure to different types of Y 2 O 3 NPs on human foreskin fibroblast cells and demonstrated a concentration-dependent (25-500 µg/mL) cytotoxicity by live/dead cell assay. 10 To investigate the potential mechanisms of the Y 2 O 3 NPs toxicity, we exposed the HEK293 cells to different concentrations of NPs to first determine the acute cytotoxic dose (IC 50 ).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells

Selvaraj¹,

Bodapati²,

Murray³

et al. 2014

IJN

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Background The increased use of engineered nanoparticles (NPs) has caused new concerns about the potential exposure to biological systems and the potential risk that these materials may pose on human health. Here, we examined the effects of exposure to different concentrations (0–50 μg/mL) and incubation times (10 hours, 24 hours, or 48 hours) of yttrium oxide (Y 2 O 3 ) NPs on human embryonic kidney (HEK293) cells. Changes in cellular morphology, cell viability, cell membrane integrity, reactive oxygen species levels, mitochondrial membrane potential, cell death (apoptosis and necrosis), and the DNA damage after NP exposure were compared to the effects seen following incubation with paraquat, a known toxicant. Results The 24-hour inhibitory concentration 50 (IC 50 ) of Y 2 O 3 NPs (41±5 nm in size) in the HEK293 cells was found to be 108 μg/mL. Incubation with Y 2 O 3 NPs (12.25–50 μg/mL) increased the ratio of Bax/Bcl-2, caspase-3 expression and promoted apoptotic- and necrotic-mediated cell death in both a concentration and a time-dependent manner. Decreases in cell survivability were associated with elevations in cellular reactive oxygen species levels, increased mitochondrial membrane permeability, and evidence of DNA damage, which were consistent with the possibility that mitochondria impairment may play an important role in the cytotoxic response. Conclusion These data demonstrate that the Y 2 O 3 NP exposure is associated with increased cellular apoptosis and necrosis in cultured HEK293 cells.

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“…For formation of curcumin loaded chitosan nanoparticles, varied concentrations of curcumin were added in the organic phase along with lecithin. Alginate coated lecithin/chitosan nanoparticles (ALG-LCN) were prepared by the slight modifications in the met hod described by Bagre et al and Lie et al [18][19] Briefly 10 mg/ml concentration sodium alginate stock solution was prepared. The curcumin loaded and blank lecithin/ chitosan nanoparticles after preparation were subjected to ultracentrifugation at 5000 rpm speed (REMI, India) for 4 h. The centrifugation at this controlled speed allowed formation of LCN concentrated suspension layer near bottom of centrifuge tubes and clearer solution at the top.…”

Section: Preparation Of Loaded and Unloaded Lecithin/ Chitosan Nanopamentioning

confidence: 99%

Alginate-chitosan Coated Lecithin Core Shell Nanoparticles for Curcumin: Effect of Surface Charge on Release Properties and Biological Activities

Pathak¹,

Amrutanand²,

Agrawal³

2017

IJPER

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Alginate-chitosan coated lecithin nanoparticles loaded with Curcumin have been prepared by layer deposition of alginate on lecithin/chitosan nanoparticles and were characterized. The prepared nanoparticles were physicochemically characterized to ensure alginate coating. The encapsulation efficiency and loading of the active nutraceutical were assessed using spectrophotometric measurements. The biological activities of the prepared nanoparticles were assessed by membrane stabilization effect of the nanoparticles on goat RBCs under hypotonic and thermally induced conditions. The biocompatibility of blank nanocarriers was assessed by performing In vitro cytotoxicity studies on HEK cell lines. The release profile of curcumin from alginate uncoated and alginate coated nanoparticles were compared to analyze effect of surface charge of the particles on studied bioactivities. It was found that uniformly sized alginate coated nanoparticles were formed with negative surface charge. It was also observed that there was no significant difference due to surface charge of the nanoparticles on cytotoxicity and membrane stabilization activity. However alginate coating offered more controlled release of the curcumin in acidic conditions suggesting that these alginate coating imparted enteric coating character.

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Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles

Cited by 170 publications

References 28 publications

In vitro and in vivo toxicity assessment of nanoparticles

In vitro and in vivo toxicity assessment of nanoparticles

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells

Alginate-chitosan Coated Lecithin Core Shell Nanoparticles for Curcumin: Effect of Surface Charge on Release Properties and Biological Activities

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