Daily BlankCell Procedure in Elecsys 2010 Improves the Reproducibility of Thyrotropin, Free Thyroxine, and Triiodothyronine

Navarro, Miguel; Palencia, Teresa; Bonnin, M R; Rosel, Pilar

doi:10.1093/clinchem/44.8.1772

Cited by 8 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 A repertoire of assays has been employed to directly measure FT4 concentration in human serum, including equilibrium dialysis, 4 ultrafiltration, 5 radioimmunoassay (RIA), 6 enzyme immunoassay (EIA), 7 time-resolved fluoroimmunoassay (TRFIA), 8 chemiluminescence immunoassay (CLIA) 9 and electrochemiluminescence immunoassay (ECLIA). 10 Labelled immunoassays, such as RIA, TRFIA, CLIA and ECLIA, are the most popular methods that have currently been used in clinical laboratories for FT4 determination. Based on the assay principle, labelled immunoassays for FT4 detection may be generally divided into three categories, namely, two-step method, 11 one-step labelled analog method 7,12 and one-step labelled-antibody method, 13,14 respectively.…”

Section: Introductionmentioning

confidence: 99%

Labelled antibody-based one-step time-resolved fluoroimmunoassay for measurement of free thyroxine in serum

et al. 2011

View full text Add to dashboard Cite

Background: Valid assays measuring free thyroxine (FT4) must perform without bias despite large variations in the concentrations and affinities of serum thyroxine-binding proteins in the population. We developed a new, rapid one-step labelled-antibody time-resolved fluoroimmunoassay (TRFIA) for FT4. Methods: Based on the heterologous combination of anti-T4 monoclonal antibody and triiodothyronine -immunoglobulin G conjugate, a one-step TRFIA for FT4 detection was established and compared with the two-step DELFIA w Free Thyroxine Assay. Matrix interference caused by endogenous binders and exogenous non-esterified fatty acids (NEFA) was also accessed in the proposed assay. Results: The developed method generally took only one hour, had a detection limit of 0.6 pmol/L and a large linear range of 2.5 -120 pmol/L. The inter-and intra-assay coefficients of variation were 3.5 -6.6% and 4.4-9.8%, respectively. Results from 110 specimens showed apparent agreement with that from the DELFIA w FT4 Assay with the square of the correlation coefficient of 0.975. This assay indicated that there was no significant dependence on endogenous binders and displayed potential interference by exogenous NEFA up to 5 mmol/L. Conclusions: The proposed one-step heterologous TRFIA FT4 assay possesses simplicity, accuracy, high sensitivity and exhibits great potential for FT4 measurement. The combination of heterologous immunoassay with TRFIA may be advantageous for FT4 immunoassay development.

show abstract

Section: Introductionmentioning

confidence: 99%

Labelled antibody-based one-step time-resolved fluoroimmunoassay for measurement of free thyroxine in serum

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Various methods have been employed to the determination of FT4 in serum, such as equilibrium dialysis 3 - 7 , ultrafiltration 8 , chromatography on polyacrylamide gel 9 , 10 or Sephadex 11 , radioimmunoassay (RIA) by use of the radioactive isotope 125 I 12 - 14 , enzyme immunoassay 15 - 17 , time-resolved fluorescence 18 , bioluminescent immunoassay 19 , fluoroimmunoassay 20 - 23 , ,chemiluminescence immunoassay 24 , 25 , electrochemiluminescence immunoassay 26 , 27 , and mass spectrometry 28 , 29 . These methods have disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum

Wang¹,

Chen²,

Lin³

et al. 2007

Int. J. Biol. Sci.

View full text Add to dashboard Cite

A microplate chemiluminescence enzyme immunoassay (CLEIA) with high sensitivity, selectivity and reproducibility was developed for the determination of free thyroxine (FT4) in human serum. A competitive assay has been utilized with horseradish peroxidase (HRP) labeled thyroxine analog in the chemiluminescence (CL) detection. The CL signal produced by the emission of photons from luminol was directly proportional to the amount of analyte. The linear range was 0.45-7.5 ng dL -1 and the detection limit was 0.09 ng dL -1 . Experimental conditions, such as temperature, pH, incubation time, titration level and other relevant variables upon the CL signal have been examined and optimized. A coefficient of variance of less than 16% was obtained for intra-and inter-assay precision. The present method has been successfully applied to the analysis of FT4 in human serum. The positive and negative coincidence ratios are satisfactory. Good correlations were obtained between the results by the proposed method and radioimmunoassay (RIA), as well as a Bayer ACS-180SE detection system.

show abstract

Comparative study on determination of plasma thyroid hormones by chemiluminescence and electrochemiluminescence immunoassay methods in sheep

et al. 2010

View full text Add to dashboard Cite

Daily BlankCell Procedure in Elecsys 2010 Improves the Reproducibility of Thyrotropin, Free Thyroxine, and Triiodothyronine

Cited by 8 publications

References 4 publications

Labelled antibody-based one-step time-resolved fluoroimmunoassay for measurement of free thyroxine in serum

Labelled antibody-based one-step time-resolved fluoroimmunoassay for measurement of free thyroxine in serum

Development of a highly sensitive and selective microplate chemiluminescence enzyme immunoassay for the determination of free thyroxine in human serum

Comparative study on determination of plasma thyroid hormones by chemiluminescence and electrochemiluminescence immunoassay methods in sheep

Contact Info

Product

Resources

About