Damage of erythrocyte by phenoxyacetic herbicides and their metabolites

Duchnowicz, Piotr; Koter, Maria; Duda, Wirgiliusz

doi:10.1016/s0048-3575(02)00139-6

Cited by 48 publications

(27 citation statements)

References 22 publications

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“…As described earlier, the major toxic pollutant in distillery effluent are phenols and heavy metals. Phenols are known to oxidise haemoglobin (Bukowska and Kowalska 2003) and provoke haemolysis of the cell (Duchnowicz et al 2002). Heavy metal exposure affected haematology almost similarly (Reddy at el.…”

Section: Resultsmentioning

confidence: 99%

Impact of Distillery Soil Leachate on Haematology of Swiss Albino Mice (Mus musculus)

Sharma

Singh

et al. 2007

Bull Environ Contam Toxicol

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This study reports significant alterations in various haematological parameters such as red and white blood corpuscles counts, haemoglobin content and packed cell volume in adult Swiss albino mice orally administered with diluted distillery soil leachate (5%-20%) for 30 days. Soil leachate also affected red blood cell morphology (poikilocytosis). The haematology of exposed mice improved in the reversal groups. Present study infers contamination potential of distillery soil leachate in the groundwater.

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Section: Resultsmentioning

confidence: 99%

Impact of Distillery Soil Leachate on Haematology of Swiss Albino Mice (Mus musculus)

Sharma

Singh

et al. 2007

Bull Environ Contam Toxicol

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“…Moreover, Duchnowicz et al (2002) proved that phenoxyherbicides, and 2,4,5-T-Na in particular, induced hemolysis in human erythrocytes. In this work we observed an increase in the size and shape (the increase of FSC-A and SSC-A values) of the erythrocytes incubated with 2,4-D-Na and MCPA-Na (Fig.…”

Section: Discussionmentioning

confidence: 96%

“…Our previous studies have proven that phenoxyherbicides changed the activity of antioxidant enzymes, e.g., superoxide dismutase, glutathione peroxidise, catalase (Bukowska 2006), and membrane acetylcholinesterase (Bukowska & Hutnik 2006). It was also revealed that these compounds oxidized haemoglobin (Duchnowicz et al 2002). Moreover, previous studies showed that phenoxyherbicides provoked oxidative damage and induced reactive oxygen species formation in human erythrocytes (Bukowska et al 2008).…”

Section: Introductionmentioning

confidence: 95%

“…To explain the mechanism of phenoxyherbicides action, we used high concentrations of these substances (ranging from 0.5 to 2 mM), which may penetrate human cells only as a result of acute poisoning. The chosen doses corresponded to the amounts of 2,4-D, 2,4-5-T and MCPA, which induced changes of various biochemical parameters studied in investigations of other authors (Suwalsky et al 1996;Kaioumova et al 2001;Duchnowicz et al 2002Duchnowicz et al , 2005Tuschl & Schwab 2003;Maire et al 2007). …”

Section: Introductionmentioning

confidence: 99%

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Comparison of the effect of phenoxyherbicides on human erythrocyte membrane (in vitro)

et al. 2011

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The molecular mechanisms of phenoxyherbicides action in animals have been insufficiently studied. Now, we have investigated the interaction of sodium salts of phenoxyherbicides, e.g., 2,4-dichlorophenoxyacetic acid (2,4-D-Na), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T-Na) and 4-chloro-2-methylphenoxyacetic acid (MCPA-Na) with human erythrocytes. In this study, we evaluated the effect of these compounds on erythrocyte membrane fluidity as well as changes in membrane proteins content. It was observed that all of the compounds studied altered membrane fluidity, changed the size and shape of the erythrocytes and provoked echinocytes formation. It was also revealed that 2,4-D-Na and 2,4,5-T-Na changed the content of erythrocyte membrane proteins mainly by a decrease in the level of spectrin and low molecular weight proteins. The comparison of the action of phenoxyherbicides examined showed that 2,4,5-T-Na caused the greatest changes in the erythrocytes membrane, whereas MCPA-Na induced the lowest alterations in the incubated cells. It must be noted that changes of the investigated parameters were observed only at presence of significant concentrations of these compounds that may penetrate human organism only as a result of acute poisoning.

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“…In fact, the pro-oxidative action of these compounds is strongly dependent on the localization of the substituent in the phenol ring. Indeed, the compounds with chlorine residues in the second and fourth position of phenol ring cause strong damage to antioxidative enzymes and lipid peroxidation (Bukowska, 2003;Bukowska et al, 2000;Duchnowicz et al, 2002). Also, they much more easily penetrate the cell membrane.…”

Section: In Vitro Studiesmentioning

confidence: 99%