Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells

Wang, Hui; Li, Minjing; Pan, Zhaohai; Li, Defang; Zheng, Qi

doi:10.3892/ol.2021.12714

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…Regarding daphnoretin, it has been mentioned that much of the research on this compound focuses on its anticancer activity. For example, in a recent report (2021), its ability to induce mitochondrial apoptosis and inhibit the proliferation of human (A375) and mouse (B16) malignant melanoma cells was shown through the activation of the production of reactive species of oxygen (ROS) [30] . Also, in 2007 daphnoretin (1.875, 3.75, 7.5, and 15 mg kg −1 ) administered orally to mice showed to cause the animals to spend more time in the open arms of an elevated plus maze, indicating that this coumarin has effects on the central nervous system that is manifested as a decrease in anxiety‐like behavior [8]…”

Section: Resultsmentioning

confidence: 99%

Anti‐Inflammatory and Neuroprotective Effects of Standardized Fractions in Herniarin and Daphnoretin from Distictis buccinatoria

Porras-Dávila

Zamilpa²,

Jiménez-Ferrer³

et al. 2023

Chemistry & Biodiversity

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Distictis buccinatoria is used for inflammatory‐related diseases. From a dichloromethane extract were obtained five different fractions called F1 to F5, and sub‐fractions F4‐1, F5‐1, F5‐2, and F5‐3; and were evaluated as anti‐neuroinflammatory, antioxidant, and nootropic agents in mice exposed to lipopolysaccharide. Also, were isolated herniarin, daphnoretin, and fraction's terpenes with an anti‐inflammatory activity using 12‐O‐tetradecanoylphorbol‐13‐acetate‐induced auricular edema. The inhibition of local edema was: F1 (73.6 %), F2 (57 %), F3 (62.61 %), F4 (87.3 %), and F5 (93.57 %). Terpene fraction inhibited at 89.60 %, herniarin at 86.92 % (Emax 99.01 %, ED50 0.35 mg ear−1), and daphnoretin at 86.41 %. Fractions F4‐1 and F5‐2 (10 mg kg−1) enhancer spatial memory acquisition and spontaneous motor activity; reduced IL‐1β, TNF‐α, and IL‐6; increased IL‐10, enhanced the activity of CAT and GR. D. buccinatoria has neuroprotective activity and contains daphnoretin and herniarin, with anti‐inflammatory activity.

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Section: Resultsmentioning

confidence: 99%

Anti‐Inflammatory and Neuroprotective Effects of Standardized Fractions in Herniarin and Daphnoretin from Distictis buccinatoria

Porras-Dávila

Zamilpa²,

Jiménez-Ferrer³

et al. 2023

Chemistry & Biodiversity

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“…It has multiple pharmacological effects of antitumor. Previous studies have found that Daphnoretin could inhibit the proliferation, migration and viability of colon cancer cells [ 11 ], malignant melanoma cells [ 12 ], lung cancer cells [ 13 ] and cervical cancer cells [ 14 ] in vitro . And it can regulate the differentiation and maturation of DCs by mediating p-JUk activity [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn

Wu,

Lin,

Chen

2024

Aging

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Objective: Spinal cord injury (SCI) is a devastating disease for which there is no safe and effective treatment at present. Daphnoretin is a natural discoumarin compound isolated from Wikstroemia indica with various pharmacological activities. Our study aimed to investigate the role of Daphnoretin in NF-κB pathway activation and inflammatory response after SCI. Methods: A mouse SCI model was constructed, and the Basso Mouse Scale Score and subscore were used to evaluate the effect of Daphnoretin on the movement capacity of mice. The effect of Daphnoretin on the activation of glial cells in the mouse model and BV2 cells was observed by immunofluorescence. PCR and ELISA were used to detect the expression of inflammatory factors, and Western blot was performed to detect the protein expression associated with NF-κB pathway. Results: Daphnoretin inhibited the loss of movement ability and the activation of glial cells in mice after SCI, and it also inhibited the activation of NF-κB pathway and the expression of inflammatory factors TNF-α and IL-1β in vivo and in vitro . Conclusions: Daphnoretin can inhibit the activation of NF-κB pathway and the inflammatory response induced by SCI. Our study demonstrates the potential of Daphnoretin on clinical application for the treatment of SCI.

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“…C. A. Meyer), belonging to the Wikstroemia genus of the Thymelaeaceae family, was a traditional Chinese medicine, reporting with a variety of antitumor active compounds such as daphnoretin (DAP) (Shao et al, 2020;Shi et al, 2021). DAP, a dicoumarin derivative, could inhibit various cancer cell growth, especially inducing HCC apoptosis via an increase in Bax and p53 expression and a decrease in Bcl2 expression (Yu et al, 2019;Badawy et al, 2021;Wang et al, 2021;Yao et al, 2021). However, DAP exhibited low water solubility and rapid clearance from the bloodstream, thus resulting in a short T1/2 in the plasma (3.5 h) (Hu et al, 2017;Liang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

A nano-preparation approach to enable the delivery of daphnoretin to potentiate the therapeutical efficacy in hepatocellular cancer

Zhu

Wang²,

Feng³

et al. 2022

Front. Pharmacol.

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Daphnoretin (DAP), isolated from a traditional Chinese medicine Wikstroemia indica (Linn. C. A. Meyer), could induce apoptosis of hepatocellular cancer (HCC) and inhibit tumor growth. However, the application of DAP in cancer therapies was hampered because to its poor solubility. Herein, this study aimed to design an approach of double-targeted nano-preparation to enable the delivery of DAP to potentiate the therapeutical efficacy in liver cancer via glycyrrhetinic acid-polyethylene glycol-block-poly (D,L-lactic acid)/polyethylene glycol-block-poly (D,L-lactic acid)-DAP (GPP/PP-DAP). In particular, the purity of separated DAP was up to 98.12% for preparation research. GPP/PP-DAP was successfully prepared by the thin-film hydration method. Subsequently, the GPP/PP-DAP was optimized by univariate analysis and the response surface methodology, producing a stable and systemically injectable nano-preparation. Impressively, on the one hand, cytotoxicity studies showed that the IC50 of the GPP/PP-DAP was lower than that of free DAP. On the other hand, the GPP/PP-DAP was more likely to be endocytosed by HepG2 cells and targeted to the liver with orthotopic tumors, potentiating the therapeutical efficacy in HCC. Collectively, both in vitro and in vivo results indicated the excellent tumor inhibition and liver targeting of GPP/PP-DAP, suggesting the nano-preparation could serve as a potential drug delivery system for natural ingredients with anti-hepatoma activity to lay the theoretical foundation for clinical application.

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Daphnoretin induces reactive oxygen species‑mediated apoptosis in melanoma cells

Cited by 4 publications

References 42 publications

Anti‐Inflammatory and Neuroprotective Effects of Standardized Fractions in Herniarin and Daphnoretin from Distictis buccinatoria

Anti‐Inflammatory and Neuroprotective Effects of Standardized Fractions in Herniarin and Daphnoretin from Distictis buccinatoria

Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn

A nano-preparation approach to enable the delivery of daphnoretin to potentiate the therapeutical efficacy in hepatocellular cancer

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