Dark fermentative hydrogen production from xylose by a hot spring enrichment culture

Feasibility of enriched mixed cultures obtained by repeated batch transfer in continuous hydrogen fermentation

“…Therefore, this study used a synthetic medium containing galactose as a sole sugar. Additionally, previous investigations reported that enriched mixed cultures showed significant improvement in hydrogen production due to the selective population of the hydrogen producers [16,17]. However, most reported investigations were conducted in batch mode operation only [18,19].…”

Effects of anode potentials on bioelectrogenic conversion of xylose and microbial community compositions

“…Duplicate samples from the anode solution and biofilm were taken from the end points of the MFC runs and the samples were stored at −20 • C. DNA extraction, polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE), sequencing and analysing of partial 16S rRNA genes were done as described earlier by Mäkinen et al [16].…”

Significance of acclimatization for biohydrogen production from synthetic lignocellulose hydrolysate in continuous-flow systems

“…Lignocellulosic substances are the most plentiful raw materials in nature [3]. Lignocellulosic material residues including pulp-and-paper wastes, food processing wastes, wheat-straw or rice-straw, corn stovers, and sugar cane bagasse [4] are produced at an annual rate of 8.15 * 10 7 tonnes worldwide [5].…”

“…All lignocellulosic materials that basically consist of 35%e45% cellulose (a polymer of glucose), 25%e40% hemicellulose (heteropolymer of hexose and pentose) and 20%e35% lignin (an aromatic organic compound) are often hydrolyzed by acid treatment. The hydrolysate can be utilized for biological hydrogen production [3] as well as bioethanol production through dark anaerobic fermentation [6]. Because lignocellulose hydrolysates contain not only glucose, but also various monosaccharides, such as xylose and arabinose [7], as well as disaccharides, such as cellobiose [8], microorganisms can efficiently ferment these sugars for biohydrogen production.…”

“…Because lignocellulose hydrolysates contain not only glucose, but also various monosaccharides, such as xylose and arabinose [7], as well as disaccharides, such as cellobiose [8], microorganisms can efficiently ferment these sugars for biohydrogen production. Dark fermentative hydrogen production using carbohydrates [3,9,10] and lignocellulosic wastes [5,11,12] by various pure and mixed cultures has been extensively studied.…”