Data of The Expression of Serotonin in Alzheimer's Disease (AD) Rat Model Under Treatment of Ethanolic Extract Ocimum sanctum Linn

Raditya, Muhammad Nova; Bagus, A.M. Made; Kustiati, Ulayatul; Wihadmadyatami, Hevi; Kusindarta, Dwi Liliek

doi:10.1016/j.dib.2020.105654

Cited by 3 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is in line with the previous studies showed that EEOS maintained the stability of several neurotransmitter, such as ChAT in human cerebral microvascular endothelial cells and HEK-293 cells as in vitro model neurodegeneration induced by TMT. (13,14,20,21) In addition, EEOS reduced nuclear fragmentation in a concentration-dependent manner (22,23) and neuronal loss have a crucial role in the development of AD. (24) Apoptosis is a cellular process that regulates cell death under physiological or pathological stimuli.…”

Section: Discussionmentioning

confidence: 99%

“…(12) Previous studies have shown that in vitro and in vivo administration of ethanolic extract of O. sanctum (EEOS) on the neurodegeneration model could promote neuron proliferation in the hippocampal cornu ammonis 1 and 3 and maintain the stability of the expression of several neurotransmitters, such as neuropeptide Y, serotonin, acetylcholinesterase (Ach) and choline acetyltransferase (ChAT). (13)(14)(15) Therefore, this study was aimed to elucidate the anti-apoptotic effects of EEOS on PC12 and SH-SY5Y cell lines as well as interaction between main active compounds of EEOS and Aβ peptide through in silico molecular docking.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ocimum sanctum Leaves Prevent Neuronal Cell Apoptosis Through Reduction of Caspase-3 and -9 Expressions and Inhibition of β-amyloid Oligomerization

Aliffia,

Ramadhani,

Wasityastuti

et al. 2023

Indones Biomed J

View full text Add to dashboard Cite

BACKGROUND: Neurodegenerative diseases are characterized by the loss of neuronal function in the nervous system. In recent years, more than 45 million people worldwide have suffered from progressive loss of memory and cognitive functions caused by Alzheimer’s disease. Ocimum sanctum is one of the medicinal plants known to have neuroprotective abilities. This study was conducted to elucidate the anti-apoptotic effects of ethanolic extract of O. sanctum (EEOS) on PC12 and SH-SY5Y cells as well as interaction between main compounds of EEOS and β-amyloid (Aβ) peptide through in silico molecular docking.METHODS: The viability of TMT-induced PC12 and SH-SY5Y cells was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and acridine orange/propidium iodide staining. Cell proliferation rate was measured with cell counting kit-8 (CCK-8) assay. Nuclear fragmentation was observed with Hoechst 33342 staining. Caspase -3 and -9 expressions were measured using enzyme-linked immunosorbent assay. Interactions between main compounds of EEOS and Aβ were visualized with in silico molecular docking.RESULTS: EEOS had the potential effect of maintaining cell viability, preventing the cell’s morphological changes, and inhibiting apoptosis via the caspase pathway in PC12 and SH-SY5Y cells. Meanwhile, flavonoid K, phenol, eugenol could interact with the active site of Aβ through hydrogen-bonding and hydrophobic interactions.CONCLUSION: EEOS could prevent neuronal cell apoptosis via downregulation of caspase-3 and -9. Main compounds of EEOS could interact with the active site of Aβ, and thereby might inhibit Aβ oligomerization. Thus, EEOS and its main compounds could be potential as neuroprotective agents for preventing neurodegenerative diseases.KEYWORDS: Ocimum sanctum, anti-apoptotic, β-amyloid, caspase, neurodegeneration

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ocimum sanctum Leaves Prevent Neuronal Cell Apoptosis Through Reduction of Caspase-3 and -9 Expressions and Inhibition of β-amyloid Oligomerization

Aliffia,

Ramadhani,

Wasityastuti

et al. 2023

Indones Biomed J

View full text Add to dashboard Cite

show abstract

“…is a traditional medicine commonly found in Indonesia and Asian countries. Various species of Ocimum are known to provide many health benefits, including anti-inflammatory, anti-fatigue, antitussive, antiseptic, antispasmodic, neuroprotective, and neuroproliferative activities [6][7][8][9][10][11], but until recently, their active mechanisms, safety, and dosage have not been determined.…”

Section: Introductionmentioning

confidence: 99%

In silico molecular docking and in vitro analysis of ethanolic extract Ocimum sanctum Linn.: Inhibitory and apoptotic effects against non-small cell lung cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background and Aim: Lung cancer, especially non-small cell lung cancer (NSCLC), has been identified as the leading cause of cancer deaths worldwide. The mortality rate from lung cancer has been estimated to be 18.4%. Until now, conventional treatments have not yielded optimal results, thus necessitating an investigation into the use of traditional herbal plants as potential candidates for its treatment. This study aimed to determine the inhibitory and apoptotic activity of the ethanolic extract from Ocimum sanctum Linn. (EEOS) by in silico molecular docking and through in vitro studies using NSCLC cells (A549 cell line). Materials and Methods: Dried simplicia of Ocimum sanctum was converted into EEOS using the maceration method. Spectrophotometry was then employed to analyze the EEOS compound. The known main active compounds were further analyzed for inhibitory and apoptotic effects on gene signaling using in silico molecular docking involving the downloading of active compounds from PubChem and target proteins from the Protein Data Bank; the active compounds and proteins were then prepared using the Discovery Studio software v. 19.0.0 and the PyRX 0.8 program, interacted with the HEX 8.0.0 program, and visualized with the Discovery Studio Visualizer v. 19.0. Finally, an in vitro analysis was performed using an antiproliferative-cytotoxic test (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay in the NSCLC A549 cell line). Results: The analysis revealed that the active compounds in the ethanolic extract were dominated by quercetin (flavonoids) (47.23% b/b) and eugenol (phenolic) (12.14% b/b). These active compounds interacted with the active sites (residual amino acids) of the αvβ3 integrin, α5β1 integrin, caspase-3, caspase-9, and vascular endothelial growth factor. Hydrogen bonds and Pi-cation and Pi-alkyl interactions were involved in the relationships between the active compounds and the active sites and thus may reveal an antioxidant property of the extract. Furthermore, in vitro analysis showed the inhibitory and antiproliferative effects of the EEOS against non-small cell cancer (A549). Conclusion: Taken together, our data showed the ability of EEOS as an inhibitor and apoptotic agent for lung cancer; however, further research is needed to determine the exact mechanism of EEOS as an herbal medication.

show abstract

The neuroprotective effect of ethanolic extract Ocimum sanctum Linn. in the regulation of neuronal density in hippocampus areas as a central autobiography memory on the rat model of Alzheimer’s disease

Mataram

Hening

Harjanti

et al. 2021

Journal of Chemical Neuroanatomy

View full text Add to dashboard Cite

Data of The Expression of Serotonin in Alzheimer's Disease (AD) Rat Model Under Treatment of Ethanolic Extract Ocimum sanctum Linn

Cited by 3 publications

References 7 publications

Ocimum sanctum Leaves Prevent Neuronal Cell Apoptosis Through Reduction of Caspase-3 and -9 Expressions and Inhibition of β-amyloid Oligomerization

Ocimum sanctum Leaves Prevent Neuronal Cell Apoptosis Through Reduction of Caspase-3 and -9 Expressions and Inhibition of β-amyloid Oligomerization

In silico molecular docking and in vitro analysis of ethanolic extract Ocimum sanctum Linn.: Inhibitory and apoptotic effects against non-small cell lung cancer

The neuroprotective effect of ethanolic extract Ocimum sanctum Linn. in the regulation of neuronal density in hippocampus areas as a central autobiography memory on the rat model of Alzheimer’s disease

Contact Info

Product

Resources

About