Dauerzucht von Diprion pini L. (Hym.: Diprionidae) im Laboratorium unter Berücksichtigung der Fotoperiode

Eichhorn, O.

doi:10.1007/bf02342165

Cited by 38 publications

(17 citation statements)

References 3 publications

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“…All stems were cleaned and sterilized according to the method of Moore and Clark (1968). Diprion pini L. (Hymenoptera, Diprionidae) was reared in the laboratory on pine twigs, as described by Bombosch and Ramakers (1976) and Eichhorn (1976) at 25±1°C, 18·h:6·h, L:D and 70% relative humidity. The egg parasitoid Chrysonotomyia ruforum Krausse (Hymenoptera, Eulophidae) was collected in the field in France (near Fontainebleau), and in southern and central Finland.…”

Section: Methodsmentioning

confidence: 99%

Insect egg deposition induces defence responses inPinus sylvestris: characterisation of the elicitor

Hilker

Stein

Schröder

et al. 2005

Journal of Experimental Biology

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Egg deposition by the phytophagous sawfly Diprion pini L. (Hymenoptera, Diprionidae) is known to induce locally and systemically the emission of volatiles in Scots pine (Pinus sylvestris L.) that attract the egg parasitoid Chrysonotomyia ruforum Krausse (Hymenoptera, Eulophidae). The egg parasitoids kill the eggs and thus prevent damage to the plant from feeding sawfly larvae. The elicitor inducing the pine's response is known to be located in the oviduct secretion which the female sawfly applies to the eggs when inserting them into a slit in the pine needle using the sclerotized ovipositor valves. In this study we have characterized the elicitor. The elicitor was still active when isolated from the oviduct and applied directly to slits made in the pine needles. However, as soon as the oviduct secretion was dissolved in Aqua dest. and stored for 3·h at room temperature or kept frozen at -80°C, its activity was lost. In contrast, oviduct secretion kept its eliciting activity, when dissolved in Ringer solution (pH 7.2) both after storage at room temperature and after freezing. The activity of the elicitor vanished after treatment of the oviduct secretion with proteinase K, which destroyed all proteins. This suggests that the elicitor in the oviduct secretion is a peptide or protein, or a component bound to these. SDS-PAGE revealed a similar, but not identical protein pattern from hemolymph and oviduct secretion. Hemolymph itself has no eliciting effect. The elicitor in the oviduct secretion is only active when transferred to slit pine needles, since its application on undamaged needles did not induce the emission of attractive volatiles.

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Section: Methodsmentioning

confidence: 99%

Insect egg deposition induces defence responses inPinus sylvestris: characterisation of the elicitor

Hilker

Stein

Schröder

et al. 2005

Journal of Experimental Biology

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“…The sawfly D. pini (Hymenoptera, Diprionidae) was reared in the laboratory on pine branches at 20 ± 1°C, 65% r.h., and 18:6 h light/dark cycles (Bombosch and Ramakers, 1976;Eichhorn, 1976). Cocoons of the sawflies N. sertifer and G. pallida were collected in the field in southern Finland and kept at the same conditions as D. pini.…”

Section: Plant and Insect Materialsmentioning

confidence: 99%

Species-specific responses of pine sesquiterpene synthases to sawfly oviposition

et al. 2010

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“…Branches were kept in water at 10°C, L18:D6, and 70% relative humidity in a climate chamber until treatment. The pine sawfly D. pini was reared on twigs of P. sylvestris L. in the laboratory as described by Bombosch and Ramakers (1976) and Eichhorn (1976) at 25°C , L18:D6 and 70% rh.…”

Section: Plants and Insectsmentioning

confidence: 99%

Analysis of volatiles from black pine (): significance of wounding and egg deposition by a herbivorous sawfly

et al. 2004

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Dauerzucht von Diprion pini L. (Hym.: Diprionidae) im Laboratorium unter Berücksichtigung der Fotoperiode

Cited by 38 publications

References 3 publications

Insect egg deposition induces defence responses inPinus sylvestris: characterisation of the elicitor

Insect egg deposition induces defence responses inPinus sylvestris: characterisation of the elicitor

Species-specific responses of pine sesquiterpene synthases to sawfly oviposition

Analysis of volatiles from black pine (): significance of wounding and egg deposition by a herbivorous sawfly

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