Day vs. night variation in the LPS effects on toad's immunity and endocrine mediators

Titon, Stefanny Christie Monteiro; Titon, Braz; Muxel, Sandra Márcia; Figueiredo, Aymam Cobo de; Floreste, Felipe Rangel; Lima, Alan Siqueira; Gomes, Fernando Ribeiro; Assis, Vania Regina

doi:10.1016/j.cbpa.2022.111184

Cited by 7 publications

(4 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…phagocytosis activity, antibody production, cytokine release, regulation of the inflammatory response) [1,3,6,9,10,[23][24][25][26][27][28]. Interestingly, during the reproductive season and acute stress response, increased CORT shows immunostimulatory effects in anurans [7,[29][30][31][32], as described for other vertebrates.…”

Section: Introductionmentioning

confidence: 83%

Testosterone immunomodulation in free-living and captive Rhinella icterica male toads

Titon

Assis

et al. 2023

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Testosterone (T) regulates immune function, with both immunostimulatory and immunosuppressive effects on several vertebrates. We investigated the covariation between plasma T and corticosterone (CORT) levels and immunity (plasma bacterial killing ability (BKA), and neutrophil to lymphocyte ratio (NLR)) in free-living Rhinella icterica male toads inside and outside the reproductive season. We found an overall positive correlation between steroids and immune traits, with toads during the reproductive season displaying increased T, CORT and BKA. We also investigated the T transdermal application effects on T, CORT, phagocytosis of blood cells, BKA and NLR in captive toads. Toads were treated with T (1, 10 or 100 µg) or vehicle (sesame oil) for eight consecutive days. Animals were bled on the first and eighth days of treatment. Increased plasma T was observed on the first and last day of T-treatment, while increased BKA was observed following all T doses on the last day, with a positive correlation between T and BKA. Plasma CORT, NLR and phagocytosis increased on the last day for all T-treated and vehicle groups. Overall, we demonstrated a positive covariation between T and immune traits in the field and T-induced augmented BKA in captive toads, indicating a T immunoenhancing effect in R. icterica males. This article is part of the theme issue ‘Amphibian immunity: stress, disease and ecoimmunology’.

show abstract

Section: Introductionmentioning

confidence: 83%

Testosterone immunomodulation in free-living and captive Rhinella icterica male toads

Titon

Assis

et al. 2023

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

show abstract

“…temperature variation, dehydration and pollution) [13,15,[65][66][67][68] and well-established stress-induced protocols (e.g. restraint and captivity maintenance) [27,29,[69][70][71]. Interestingly, although these aforementioned stressors are distinct, their impact on amphibian immunity is generally favourable when associated with short-term stressors [16,27,30,60], contrasting with harmful effects under long-term stress conditions [28][29][30].…”

Section: Stress-related Immunomodulationmentioning

confidence: 99%

“…Considering the challenges invasive species face as they expand into novel environments with substantial seasonal changes in food availability, energy restrictions might shift their immune function toward more economical and general avenues of combating pathogens [112]. Another paper of this issue, [113] investigates a native Brazilian toad species (Rhinella icterica), to establish a positive correlation between testosterone, corticosterone and immune function in the field, and a positive correlation between testosterone and immune function in captive male toads, suggesting these steroids show an immunostimulatory effect during the reproduction in anurans [113].…”

Section: Ecoimmunologymentioning

confidence: 99%

Introduction to the special issue Amphibian immunity: stress, disease and ecoimmunology

Assis

Robert

Titon

2023

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Amphibian populations have been declining worldwide, with global climate changes and infectious diseases being among the primary causes of this scenario. Infectious diseases are among the primary drivers of amphibian declines, including ranavirosis and chytridiomycosis, which have gained more attention lately. While some amphibian populations are led to extinction, others are disease-resistant. Although the host's immune system plays a major role in disease resistance, little is known about the immune mechanisms underlying amphibian disease resistance and host–pathogen interactions. As ectotherms, amphibians are directly subjected to changes in temperature and rainfall, which modulate stress-related physiology, including immunity and pathogen physiology associated with diseases. In this sense, the contexts of stress, disease and ecoimmunology are essential for a better understanding of amphibian immunity. This issue brings details about the ontogeny of the amphibian immune system, including crucial aspects of innate and adaptive immunity and how ontogeny can influence amphibian disease resistance. In addition, the papers in the issue demonstrate an integrated view of the amphibian immune system associated with the influence of stress on immune–endocrine interactions. The collective body of research presented herein can provide valuable insights into the mechanisms underlying disease outcomes in natural populations, particularly in the context of changing environmental conditions. These findings may ultimately enhance our ability to forecast effective conservation strategies for amphibian populations. This article is part of the theme issue ‘Amphibian immunity: stress, disease and ecoimmunology’.

show abstract

“…This rodent exhibits a daily rhythm of leukocytes, peaking in the circulation during the resting period, whereas recruitment of leukocytes to tissues is higher during the active period after LPS injection (Scheiermann et al 2012, 2013). Activation of HPA axis in toads followed LPS injection is more pronounced during activity period compared with resting period (Titon et al 2022). In birds, LPS injection triggers fever response during the resting period ( Melospiza melodia ; Adelman et al 2010; Passer domesticus ; Coon et al 2011; Taeniopygia guttata ; Skold-Chiriac et al 2015), with a brief hypothermic response during the activity period ( Taeniopygia guttata ; Owen-Ashley et al 2006; Zonotrichia leucophrys gambelii ; Burness et al 2010).…”

Section: Introductionmentioning

confidence: 99%

The acute phase response in bats (Carollia perspicillata) varied with the time and dose of the immune-challenge

Viola

M.²,

Cruz‐Neto

2022

Preprint

View full text Add to dashboard Cite

The acute phase response (APR) is a core component of the innate immune response and represents the first line of immune defense used in response to infections. Although several studies with vertebrates reported fever, decrease in food intake and body mass, as well as increase in neutrophil/lymphocyte ratio and total white blood cell count after lipopolysaccharide (LPS) inoculation there was great variability in the magnitude of these responses. Some of these differences might reflect, to some extent, differences in time of endotoxin inoculation (during activity or resting periods) and dose; however, no study in the literature has evaluated the synergistic effect of these two factors in vertebrates. Therefore, our study tested the interplay between LPS dose and time of injection on selected physiological (fever and increase in total white blood cell count and neutrophil/lymphocyte ratio), and behavioral (food intake) components of APR using a Neotropical fruit-eating bat (Carollia perspicillata) as a model organism. We predicted that LPS would trigger a dose- and time-dependent response on APR components. APR components were assessed in resting and activity periods after injection of three doses of LPS (5, 10 and 15 mg/kg LPS). The results showed that LPS-evoked changes in skin temperature, food intake, neutrophil lymphocyte ratio depend markedly on the LPS dose and/or time that LPS is administered.

show abstract

Day vs. night variation in the LPS effects on toad's immunity and endocrine mediators

Cited by 7 publications

References 65 publications

Testosterone immunomodulation in free-living and captive Rhinella icterica male toads

Testosterone immunomodulation in free-living and captive Rhinella icterica male toads

Introduction to the special issue Amphibian immunity: stress, disease and ecoimmunology

The acute phase response in bats (Carollia perspicillata) varied with the time and dose of the immune-challenge

Contact Info

Product

Resources

About