Daya Antibakteri Ekstrak Batang Pisang Ambon (Musa paradisiaca var. sapientum) terhadap Pertumbuhan Enterococcus faecalis

Hafizha, H.; Suardita, Ketut; Pribadi, Nirawati

doi:10.20473/cdj.v8i2.2018.85-90

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…there is no formation (ODNC>OD), weak formation (ODNC<OD ≤ 2 × ODNC), moderate formation (2 × ODNC<OD ≤ 4 × ODNC), nor strong biofilm (OD>4 × ODNC). Based on that, the concentration extracts capable of reducing S. mutans and E. faecalis biofilms is found starting from 1.56% extract (table 5 and table 6) S. mutans and E. faecalis are grampositive bacteria with cell walls with thick peptidoglycan arrangement close to the cell surface [25]. That can cause bioactive compounds with antibacterial properties to become easily associated with the bacteria's surface, potentially damaging and hindering cell wall synthesis.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

OCTIARA,

ADINDA,

SEMBIRING

2024

Int J App Pharm

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Objective: S. mutans biofilms have become the main cause of dental caries. Meanwhile, E. faecalis biofilms cause endodontic infection. Batak leeks are native North Sumatra herb that contains antibacterial properties. This study aims to determine Batak leeks extract's antibacterial and antibiofilm effectiveness towards S. mutans and E. faecalis. Methods: This study was a laboratory experimental research with a posttest-only control group design. The sample size was 27, consisting of 3 samples each with concentrations of 0.78%; 1.56%; 3.25%; 6.25%; 12.5%; 25% and 50% Batak leek extract and 3 negative control samples and 3 positive. The Kirby-Bauer disc diffusion method was used to determine MIC, the streaking method to determine MBC and the Static Microtiter Plate Assays method for antibiofilm determination. Data analysis used Oneway ANOVA and Kruskall Wallis tests followed by post-hoc LSD and Mann-Whitney tests. Results: The results showed that Batak leeks extract contained MIC values at 0,78% concentration levels with an average inhibitory zone of 6,80±0,10 mm in S. mutans and E. faecalis. MBC values were undetected because there were no group capable of reducing bacteria at 98-98% and the highest antibiofilm reduction was found in 50% concentration extract with values of 85.58% for S. mutans and 90.29% for E. faecalis. Conclusion: It concluded that 0.78% concentration of Batak leeks extract has an antibacterial effect, but at 50% concentration was the most influential in inhibiting S. mutans and E. faecalis, and antibiofilm ability starts at the concentration level of 1.56%.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

OCTIARA,

ADINDA,

SEMBIRING

2024

Int J App Pharm

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“…If caries is left untreated, there is a risk of pulp and periapical disease which must be treated with root canal treatment (Bakhitah, et al 2021;Rumate et al, 2023). The main reason for failure of this treatment is often associated with the presence of Enterococcus faecalis (Hafizha et al, 2018;Blancas et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Antibacterial Effectiveness of Moringa Leaf Extract (Moringa oleifera) on the Growth of Enterococcus faecalis and Streptococcus mutans Bacteria in Vitro

Muharraran,

Erawati,

Dalimunthe

2023

jppipa, pendidikan ipa, fisika, biologi, kimia

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Streptococcus mutans and Enterococcus faecalis are bacterial pathogens of dental caries and root canal treatment failure. The growth of bacteria that cause dental and oral disease can be inhibited by using antibacterials derived from plants that have antibacterial properties. The aim of the research was to compare the antibacterial effectiveness of Moringa oleifera leaf extract against the growth of Enterococcus faecalis and Streptococcus mutans bacteria in vitro. This type of research is a laboratory experiment with a post test only control group design. The research samples were pure cultures of Streptococcus mutans and Enterococcus faecalis. There were seven treatment groups, namely Moringa leaf extract concentrations of 50%, 25%, 12.5%, 6.25%, 3.125%, positive control chlorhexidine and negative control DMSO with four repetitions each. Testing the antibacterial effectiveness of Moringa leaf extract used the diffusion and dilution method, then the data was analyzed using one way ANOVA and post hoc LSD statistical tests. Based on the research results, it was stated that there was effective inhibitory and killing power of Moringa oleifera leaf extract on the growth of Enterococcus faecalis and Streptococcus mutans bacteria. From the research results, it can be concluded that Moringa oleifera leaf extract is more effective as an antibacterial against Streptoccus mutans compared to Enterococcus faecalis

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Antibacterial Activity of Durian Peel Ethanol Extract (Durio zibethinus Murr.) against Streptococcus mutans and Enterococcus faecalis

Octiara,

Meliala,

Sikumbang

2023

Biomed. Pharmacol. J.

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Dental caries and root canal infections are common dental and oral diseases due to the dominance of S.mutans and E.faecalis. Despite not having any economic value and becoming a waste, durian peel is claimed as an antibacterial. This study aims to analyze durian peel ethanol extract antibacterial activity against S.mutans and E.faecalis based on the inhibition zone diameter, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). This type of research is a laboratory experiment using Post-test Only Control Group Design. There were 7 concentrations of durian peel ethanol extract (50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78%), both positive and negative control with three times repetitions. Moreover, the bacterial suspensions used were S.mutans ATCC 25175 and E.faecalis ATCC 29212. Data analysis used One Way Anova, while data that were not normally distributed used Kruskal-Wallis. Post Hoc analysis used LSD for normal data and Mann-Whitney for abnormal data. Durian peel ethanol extract shows the antibacterial activity of S.mutans with MIC at a concentration of 0.78%, while against E.faecalis is 12.5%. The higher the concentration, the larger the inhibition zone formed. The best-tested concentration of ethanol extract from durian peel is 6.25-50% to inhibit the growth of S. mutans and 50% for E.faecalis. Durian peel ethanol extract can prevent dental caries since it is antibacterial against S.mutans and E.faecalis.

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Daya Antibakteri Ekstrak Batang Pisang Ambon (Musa paradisiaca var. sapientum) terhadap Pertumbuhan Enterococcus faecalis

Cited by 3 publications

References 16 publications

Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

Effectiveness of Batak Leeks Extract Against S. Mutans and E. Faecalis as Antibacterial and Antibiofilm Agent

Comparison of the Antibacterial Effectiveness of Moringa Leaf Extract (Moringa oleifera) on the Growth of Enterococcus faecalis and Streptococcus mutans Bacteria in Vitro

Antibacterial Activity of Durian Peel Ethanol Extract (Durio zibethinus Murr.) against Streptococcus mutans and Enterococcus faecalis

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