Deciphering the relationship between caveolae-mediated intracellular transport and signalling events

Popov, Lucia-Doina

doi:10.1016/j.cellsig.2022.110399

Cited by 10 publications

(11 citation statements)

References 199 publications

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“…The key proteins that contribute to caveolae-mediated endocytosis are caveolins, which provide a scaffold along with adaptor proteins, cavin, and many stabilizing molecules. 165 Caveolae endocytose materials and localize it into caveosomes, which can either be taken up by the Golgi body (GB) and endoplasmic reticulum (ER) for delivery or be exocytosed without engaging the hydrolytic environment of lysosomes. 166 Previously, it was reported that anionic nanoparticles use caveolae-mediated endocytosis pathways.…”

Section: Caveolae-mediated Endocytosismentioning

confidence: 99%

“…The endocytic function of caveolae is mediated by a complex mechanism of caveolae formation, assembly, detachment, and intracellular trafficking (Figure ). The key proteins that contribute to caveolae-mediated endocytosis are caveolins, which provide a scaffold along with adaptor proteins, cavin, and many stabilizing molecules . Caveolae endocytose materials and localize it into caveosomes, which can either be taken up by the Golgi body (GB) and endoplasmic reticulum (ER) for delivery or be exocytosed without engaging the hydrolytic environment of lysosomes .…”

Section: Caveolae-mediated Endocytosismentioning

confidence: 99%

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Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

2023

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Oral drug administration has been a popular choice due to patient compliance and limited clinical resources. Orally delivered drugs must circumvent the harsh gastrointestinal (GI) environment to effectively enter the systemic circulation.The GI tract has a number of structural and physiological barriers that limit drug bioavailability including mucus, the tightly regulated epithelial layer, immune cells, and associated vasculature. Nanoparticles have been used to enhance oral bioavailability of drugs, as they can act as a shield to the harsh GI environment and prevent early degradation while also increasing uptake and transport of drugs across the intestinal epithelium. Evidence suggests that different nanoparticle formulations may be transported via different intracellular mechanisms to cross the intestinal epithelium. Despite the existence of a significant body of work on intestinal transport of nanoparticles, many key questions remain: What causes the poor bioavailability of the oral drugs? What factors contribute to the ability of a nanoparticle to cross different intestinal barriers? Do nanoparticle properties such as size and charge influence the type of endocytic pathways taken? In this Review, we summarize the different components of intestinal barriers and the types of nanoparticles developed for oral delivery. In particular, we focus on the various intracellular pathways used in nanoparticle internalization and nanoparticle or cargo translocation across the epithelium. Understanding the gut barrier, nanoparticle characteristics, and transport pathways may lead to the development of more therapeutically useful nanoparticles as drug carriers.

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Section: Caveolae-mediated Endocytosismentioning

confidence: 99%

Section: Caveolae-mediated Endocytosismentioning

confidence: 99%

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

2023

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“…[20,46] However, transferrin receptors have been reported to be present in caveolae-associated pathways as well. [47] Over the years, several studies investigated clathrin-mediated endocytosis of both liposomes and EVs, which we will compare in detail below. The main results of these studies are also summarized in Table S2 (Supporting Information).…”

Section: Clathrin-dependent Endocytosismentioning

confidence: 99%

“…At the site of this particular invagination, membrane domains are rich in glycolipids, sphingolipids, and cholesterol (see Figure 3-1). [47,57] The main proteins that orchestrate and stabilize caveolae-mediated endocytosis are the caveolin and cavin family, as well as the dynamin-related EH Domain Containing 2 ATPase and the BAR protein domaincontaining syndapin/Pacsin2. [58] Caveolins are hairpin-like integral membrane proteins, anchored to the plasma membrane at the cytosolic side, which bind to actin filaments, to ensure caveolae formation.…”

Section: Caveolae-dependent Endocytosismentioning

confidence: 99%

“…[58] Caveolins are hairpin-like integral membrane proteins, anchored to the plasma membrane at the cytosolic side, which bind to actin filaments, to ensure caveolae formation. [13,47,59,60] Once the invagination is initiated, cavins are recruited from the cytosol, which oligomerizes into trimers, thus promoting membrane remodeling and generating "flask"shaped invaginations (see Figure 3-2). [61] Following oligomerization, the EH Domain Containing 2 ATPase is recruited to the site of the invagination for stabilization.…”

Section: Caveolae-dependent Endocytosismentioning

confidence: 99%

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A Comparison of Cellular Uptake Mechanisms, Delivery Efficacy, and Intracellular Fate between Liposomes and Extracellular Vesicles

Gandek

Koog

Nagelkerke

2023

Adv Healthcare Materials

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A key aspect for successful drug delivery via lipid‐based nanoparticles is their internalization in target cells. Two prominent examples of such drug delivery systems are artificial phospholipid‐based carriers, such as liposomes, and their biological counterparts, the extracellular vesicles (EVs). Despite a wealth of literature, it remains unclear which mechanisms precisely orchestrate nanoparticle‐mediated cargo delivery to recipient cells and the subsequent intracellular fate of therapeutic cargo. In this review, internalization mechanisms involved in the uptake of liposomes and EVs by recipient cells are evaluated, also exploring their intracellular fate after intracellular trafficking. Opportunities are highlighted to tweak these internalization mechanisms and intracellular fates to enhance the therapeutic efficacy of these drug delivery systems. Overall, literature to date shows that both liposomes and EVs are predominantly internalized through classical endocytosis mechanisms, sharing a common fate: accumulation inside lysosomes. Studies tackling the differences between liposomes and EVs, with respect to cellular uptake, intracellular delivery and therapy efficacy, remain scarce, despite its importance for the selection of an appropriate drug delivery system. In addition, further exploration of functionalization strategies of both liposomes and EVs represents an important avenue to pursue in order to control internalization and fate, thereby improving therapeutic efficacy.

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Exosomes; multifaceted nanoplatform for targeting brain cancers

et al. 2023

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Deciphering the relationship between caveolae-mediated intracellular transport and signalling events

Cited by 10 publications

References 199 publications

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

Mechanisms of Nanoparticle Transport across Intestinal Tissue: An Oral Delivery Perspective

A Comparison of Cellular Uptake Mechanisms, Delivery Efficacy, and Intracellular Fate between Liposomes and Extracellular Vesicles

Exosomes; multifaceted nanoplatform for targeting brain cancers

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