Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer

Guo, Haonan; Zhang, Rui; Afrifa, Justice; Wang, Yuanyuan; Yu, Jingcui

doi:10.1016/j.prp.2019.03.031

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…Both effects involve complex regulatory networks, such as those mediated by p53, PI3K/AKT/mTOR, Ras, and microRNA ( Zhou et al, 2016 ). Our group has demonstrated that TOB1 is a tumor suppressor in gastric cancer ( Guan et al, 2017 ; Guo et al, 2019 ; Wang et al, 2019 ; Wang et al, 2018 ; Yu et al, 2011 ; Yu et al, 2008a ; Yu et al, 2008b ). Experimental data from our study indicated that TOB1 may induce autophagy in gastric cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…An elevated ratio of p-TOB1 was associated with functional inactivation of the TOB1 gene in gastric cancer ( Yu et al, 2011 ). Moreover, decreased expression levels of DAL-1 and TOB1 were associated with shorter survival of gastric cancer patients ( Guo et al, 2019 ). Therefore, this study evaluated whether and how the TOB1 gene induces autophagy in gastric cancer, aiming to provide more evidence for TOB1 as a potential therapeutic target in gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of TOB1 on autophagy in gastric cancer AGS cells via decreasing the activation of AKT/mTOR signaling pathway

Wang

Sui

et al. 2022

PeerJ

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Background We previously identified the tumor suppressor gene TOB1 as related to gastric cancer. The purpose of this study was to explore whether TOB1 induces autophagy through the AKT/mTOR signaling pathway in gastric cancer. Methods Western blotting was used to detect the protein levels of TOB1, LC3, AKT, mTOR, phosphorylated (p) AKT, and p-mTOR. A double fluorescent GFP-RFP-LC3 fusion protein was used to trace autophagy by laser confocal microscopy. Autophagosomes were observed by transmission electron microscopy. Results The conversion of LC3-I to LC3-II and the LC3-II/LC3-I ratio were significantly increased in AGS cells overexpressing TOB1 compared with control cells. Fluorescence imaging showed LC3 puncta at 48 h, and these puncta increased significantly at 72 h after TOB1 transfection compared with control tumor cells. The presence of autophagosomes in AGS cells was observed at 72 h after TOB1 transfection by transmission electron microscopy, and no autophagosomes were found in the control cells. Moreover, the levels of p-AKT and p -mTOR were lower in AGS cells than in control cancer cells. Conclusion Our results provide novel insight that TOB1 might suppress gastric cancer by inducing autophagy, possibly through decreasing phosphorylation and the subsequent activation of the AKT/mTOR signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Involvement of TOB1 on autophagy in gastric cancer AGS cells via decreasing the activation of AKT/mTOR signaling pathway

Wang

Sui

et al. 2022

PeerJ

Self Cite

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“…Additionally, downregulated expression of Tob1 has been found in malicious gastric cancer, suggesting that low expression of Tob1 may be an independent indicator of poor prognosis in gastric cancers [38]. Consistently, downregulation of Tob1 is associated with shorter survival of gastric cancer patients, suggesting that Tob1 may be considered a potential marker for predicting the outcomes of patients with gastric cancer [39]. To summarize the above, an anti-proliferative gene product Tob1 in APRO family tends to be induced by inflammatory stimulus of IBD.…”

Section: Anti-proliferative Family Proteins Antitumor Immunity and Co...mentioning

confidence: 91%

A budding concept with certain microbiota, anti-proliferative family proteins, and engram theory for the innovative treatment of colon cancer

Ikeda

Taniguchi

Yoshikawa

et al. 2022

Exploration of Medicine

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Inflammatory bowel disease (IBD) is a multifactorial chronic disease. Patients with IBD have an increased risk of developing colorectal cancer which has become a major health concern. IBD might exert a role of engrams for making the condition of specific inflammation in the gut. Dysregulation of immune cells induced by the command of engrams might be crucial in the pathogenesis of damages in gut epithelium. The anti-proliferative (APRO) family of anti-proliferative proteins characterized by immediate early responsive gene-products that might be involved in the machinery of the carcinogenesis in IBD. Herein, it is suggested that some probiotics with specific bacteria could prevent the development and/or progression of the IBD related tumors. In addition, consideration regarding the application of studying APRO family proteins for the comprehension of IBD related tumors has been presented. It is hypothesized that overexpression of Tob1, a member of APRO family proteins, in the epithelium of IBD could suppress the function of adjacent cytotoxic immune cells possibly via the paracrine signaling.

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“…The downregulated expression of Tob1 has been found in malignant gastric cancer, suggesting that the low expression of Tob1 may be an independent indicator of poor prognosis in gastric cancers [7]. Similarly, the downregulation of Tob1 may be associated with the shorter survival of gastric cancer patients [8]. Consistently, Tob1 overexpression could not only increase the expression of PTEN, but also regulate the downstream effectors in the PI3K/PTEN signaling pathway, leading to the suppression of cancer cell proliferation [9].…”

Section: Introductionmentioning

confidence: 99%

Presumed Roles of APRO Family Proteins in Cancer Invasiveness

Ikeda

Taniguchi

Sawamura

et al. 2022

Cancers

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The APRO family members may be involved in the regulation of cell growth, migration, and/or invasion. Although an APRO protein could suppress the invasiveness of several cancer cells, it has been reported that overexpression of the same APRO protein could also promote the invasiveness and/or metastasis of the same cancer cells. In general, the invasiveness of cancer cells might be associated with the function of matrix metalloproteinases (MMPs) as well as with the function of certain exosomes. However, it has been shown that exosomes involving particular APRO proteins, MMPs, and/or microRNA could contribute to the regulation of invasiveness. Here, we discuss contradictory reports on invasiveness in relation to APRO family proteins on the basis of understanding the function of MMPs and/or various exosomes. A better understanding of those mechanisms could be of use to bring about innovative strategies for cancer treatment.

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Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer

Cited by 9 publications

References 37 publications

Involvement of TOB1 on autophagy in gastric cancer AGS cells via decreasing the activation of AKT/mTOR signaling pathway

Involvement of TOB1 on autophagy in gastric cancer AGS cells via decreasing the activation of AKT/mTOR signaling pathway

A budding concept with certain microbiota, anti-proliferative family proteins, and engram theory for the innovative treatment of colon cancer

Presumed Roles of APRO Family Proteins in Cancer Invasiveness

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