Decreased expression of type II tumor suppressor gene RARRES3 in tissues of hepatocellular carcinoma and cholangiocarcinoma

Jiang, Shun-Yuan; Chou, Jung-Mao; Leu, Fur-Jiang; Hsu, Yu-Yen; Shih, Yu-Lung; Yu, Jyh‐Cherng; Lee, Meei-Shyuan; Shyu, Rong-Yaun

doi:10.3748/wjg.v11.i7.948

Cited by 18 publications

(17 citation statements)

References 24 publications

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“…CD38 expressed mainly in leukocytes is involved in cell adhesion and signal transduction, RARRES3 is a negative regulator of cell proliferation, whereas ECGF1 is a growth factor with angiogenic effects. RARRES3 has been reported to act as a tumor suppressor or growth regulator (46). Its decreased expression in colorectal cancer seems to support this assumption.…”

Section: Discussionsupporting

confidence: 65%

Diagnostic mRNA Expression Patterns of Inflamed, Benign, and Malignant Colorectal Biopsy Specimen and their Correlation with Peripheral Blood Results

Galamb

Sípos²,

Solymosi³

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Purpose: Gene expression profile (GEP) -based classification of colonic diseases is a new method for diagnostic purposes. Our aim was to develop diagnostic mRNA expression patterns that may establish the basis of a new molecular biological diagnostic method. Experimental Design: Total RNA was extracted, amplified, and biotinylated from frozen colonic biopsies of patients with colorectal cancer (n = 22), adenoma (n = 20), hyperplastic polyp (n = 11), inflammatory bowel disease (n = 21), and healthy normal controls (n = 11), as well as peripheral blood samples of 19 colorectal cancer and 11 healthy patients. Genome-wide gene expression profile was evaluated by HGU133plus2 microarrays. To identify the differentially expressed features, the significance analysis of microarrays and, for classification, the prediction analysis of microarrays were used. Expression patterns were validated by realtime PCR. Tissue microarray immunohistochemistries were done on tissue samples of 121 patients.Results: Adenoma samples could be distinguished from hyperplastic polyps by the expression levels of nine genes including ATP-binding cassette family A, member 8, insulin-like growth factor 1 and glucagon (sensitivity, 100%; specificity, 90.91%). Between lowgrade and high-grade dysplastic adenomas, 65 classifier probesets such as aquaporin 1, CXCL10, and APOD (90.91/100) were identified; between colorectal cancer and adenoma, 61 classifier probesets including axin 2, von Willebrand factor, tensin 1, and gremlin 1 (90.91/100) were identified. Early-and advanced-stage colorectal carcinomas could be distinguished using 34 discriminatory transcripts (100/66.67). Conclusions: Whole genomic microarray analysis using routine biopsy samples is suitable for the identification of discriminative signatures for differential diagnostic purposes. Our results may be the basis for new GEPbased diagnostic methods. (Cancer Epidemiol Biomarkers Prev 2008;17(10):2835 -45)

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Section: Discussionsupporting

confidence: 65%

Diagnostic mRNA Expression Patterns of Inflamed, Benign, and Malignant Colorectal Biopsy Specimen and their Correlation with Peripheral Blood Results

Galamb

Sípos²,

Solymosi³

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…15 The expression of TIG3 was decreased in most aggressive squamous cell carcinoma tumors, 7 as well as in hepatocellular carcinoma, cholangiocarcinoma and colorectal adenocarcinoma. 16 TIG3 expression is elevated after treating with tazarotene in vitro in primary human keratinocytes confirmed in our study and in vivo in psoriatic lesions, which may result in keratinocyte growth arrest, apoptosis and terminal differentiation. Treatment with all-trans retinoic acid can also induce TIG3 expression in head and neck carcinomas, lung carcinoma cells 17 and gastric cancer cells.…”

Section: Discussionsupporting

confidence: 86%

“…TIG3 mapped to chromosome 11q23 that was hypothesized to contain a tumor suppressor 15 . The expression of TIG3 was decreased in most aggressive squamous cell carcinoma tumors, 7 as well as in hepatocellular carcinoma, cholangiocarcinoma and colorectal adenocarcinoma 16 . TIG3 expression is elevated after treating with tazarotene in vitro in primary human keratinocytes confirmed in our study and in vivo in psoriatic lesions, which may result in keratinocyte growth arrest, apoptosis and terminal differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effects of narrow‐band ultraviolet B and tazarotene therapy on keratinocyte proliferation and TIG3 expression

Luo

Zheng

Peng

et al. 2008

The Journal of Dermatology

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Tazarotene plus narrow-band ultraviolet B (NB-UVB) therapy has been shown to enhance the efficacy in treating patients with psoriasis, while the mechanism is not clear. The present study aims to investigate the alteration of cell proliferation and TIG3 in cultured normal human keratinocytes after NB-UVB and/or tazarotene treatment. Keratinocytes were exposed to NB-UVB, then incubated with or without tazarotene, and then cell proliferation was detected by methyl thiazoleterazolium colorimetric assay and TIG3 mRNA expression and protein production was examined by real-time reverse transcription polymerase chain reaction and immunocytochemistry, respectively. The results show that keratinocyte proliferation was inhibited and TIG3 mRNA expression and protein production were elevated by tazarotene at a dose higher than 0.1 micromol/L. In NB-UVB single irradiating groups, only 200 mJ/cm2 NB-UVB inhibited keratinocyte proliferation, and none of the irradiated groups had an effect on TIG3 expression. Moreover, tazarotene plus NB-UVB have stronger effects than those separately. These results indicate NB-UVB plus tazarotene may have synergistic effects on inhibiting keratinocyte proliferation and elevating TIG3 expression, which may have some implications for the understanding of how to treat psoriasis patients with tazarotene plus NB-UVB.

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“…Studies indicated that overexpression of RIG1 suppressed the colonies formation in T47D and HaCaT cells [1,6]. Compare to normal cells, the expression of RIG1 was decreased in many human cancer specimens [7–9]. RIG1 has also been shown to facilitate apoptosis and the terminal stages in keratinocyte differentiation via activation of type I transglutaminase [6].…”

Section: Introductionmentioning

confidence: 99%

Expression of the class II tumor suppressor gene RIG1 is directly regulated by p53 tumor suppressor in cancer cell lines

et al. 2012

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Edited by Varda Rotter Keywords:Retinoid-inducible gene 1 Retinoic acid receptor responder 3 Tazarotene-induced gene 3 p53 Class II tumor suppressor a b s t r a c t Recent studies indicated that the RIG1 (RARRES3/TIG3) plays an important role in cell proliferation, differentiation, and apoptosis. However, the regulatory mechanism of RIG1 gene expression has not been clearly elucidated. In this study, we identified a functional p53 response element (p53RE) in the RIG1 gene promoter. Transfection studies revealed that the RIG1 promoter activity was greatly enhanced by wild type but not mutated p53 protein. Sequence specific mutation of the p53RE abolished p53-mediated transactivation. Specific binding of p53 protein to the rig-p53RE was demonstrated using electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay. Further studies confirmed that the expression of RIG1 mRNA and protein is enhanced through increased p53 protein in HepG2 or in H24-H1299 cells. In conclusion, our results indicated that RIG1 gene is a downstream target of p53 in cancer cell lines.

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Decreased expression of type II tumor suppressor gene RARRES3 in tissues of hepatocellular carcinoma and cholangiocarcinoma

Cited by 18 publications

References 24 publications

Diagnostic mRNA Expression Patterns of Inflamed, Benign, and Malignant Colorectal Biopsy Specimen and their Correlation with Peripheral Blood Results

Diagnostic mRNA Expression Patterns of Inflamed, Benign, and Malignant Colorectal Biopsy Specimen and their Correlation with Peripheral Blood Results

Effects of narrow‐band ultraviolet B and tazarotene therapy on keratinocyte proliferation and TIG3 expression

Expression of the class II tumor suppressor gene RIG1 is directly regulated by p53 tumor suppressor in cancer cell lines

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