Decreased sulfhydryl groups of serum albumin in coronary artery disease.

Kadota, Kazushige; Yui, Yoshiki; Hattori, Ryuichi; Murohara, Yoshiharu; Kawai, Chuichi

doi:10.1253/jcj.55.937

Cited by 46 publications

(24 citation statements)

References 12 publications

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“…Even if selective artifactual oxidation of Met to MetSO was occurring in the SLE patient samples, the decrease in total levels of Met plus MetSO and the increased loss seen with increased disease activity are still consistent with an enhanced level of oxidative stress in the SLE patients. Oxidation of Cys residues has been observed in other diseases in which oxidative stress has been implicated, including adult respiratory distress syndrome (36) and coronary artery disease (37). Previous studies have reported decreased serum thiols in SLE patients compared with controls.…”

Section: Discussionmentioning

confidence: 99%

Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

Morgan¹,

Sturgess²,

Davies³

2005

Arthritis & Rheumatism

121

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Objective. To examine protein oxidation in systemic lupus erythematosus (SLE) and to correlate levels of protein oxidation products with disease activity.Methods. Serum was collected from SLE patients and healthy control subjects. Protein-bound carbonyls and the pro-oxidant enzyme myeloperoxidase (MPO) were quantified by enzyme-linked immunosorbent assay. Protein thiols were quantified using 5,5-dithionitrobenzoic acid. Protein-bound amino acids and methionine, tyrosine, and phenylalanine oxidation products were quantified by acid hydrolysis and highperformance liquid chromatography. Disease activity was assessed by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Levels of antidouble-stranded DNA (anti-dsDNA) antibodies were measured by radioimmunoassay.Results. Compared with control subjects, SLE patients exhibited elevated levels of protein carbonyls (0.108 ؎ 0.078 versus 0.064 ؎ 0.028 nmoles/mg of protein; P ‫؍‬ 0.046), decreased levels of protein thiols (3.9 ؎ 1.1 versus 4.9 ؎ 0.7 nmoles/mg of protein; P ‫؍‬ 0.003), decreased levels of protein-bound methionine (P ‫؍‬ 0.0007), and increased levels of protein-bound methionine sulfoxide (P ‫؍‬ 0.0043) and 3-nitrotyrosine (P ‫؍‬ 0.0477). SLE patients with high SLEDAI scores or elevated anti-dsDNA antibody levels exhibited increased oxidation compared with patients with low SLEDAI scores or low antibody levels. Serum MPO levels were decreased in SLE patients (P ‫؍‬ 0.03), suggesting that this enzyme is not responsible for the enhanced protein oxidation.Conclusion. We found elevated levels of multiple markers of protein oxidation in sera from SLE patients compared with controls, and these levels correlated with disease activity. The findings suggest that protein oxidation may play a role in the pathogenesis of chronic organ damage in SLE.

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Section: Discussionmentioning

confidence: 99%

Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

Morgan¹,

Sturgess²,

Davies³

2005

Arthritis & Rheumatism

121

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“…SH groups are essential antioxidants in blood and tissue levels. They are reactive constituents of protein molecules and enzymes, including Ca +2 -ATPase, calcium channels, Na + -K + -ATPase, and creatine kinase [24]. Na + -K + -ATPase is important in the cornea to maintain the normal sodium-potassium balance, which is important for lens transparency.…”

Section: Discussionmentioning

confidence: 99%

Are cefuroxime and vancomycin really safe on the corneal endothelial cells?

Özlem

Necati

Fatma

et al. 2010

Graefes Arch Clin Exp Ophthalmol

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“…Also, interestingly Kadota, et al found that mercaptalbumin and total SH contents were reduced in proportion to the severity of CAD. 32) Usal, et al suggested that depressed GSH levels might be associated with an enhanced protective mechanism to oxidative stress in acute myocardial infarction (AMI). 33) Even though no data regarding homocysteine metabolism disturbance, total SH content or GSH levels were obtained in our study, we strongly suspect that CAD patients might be affected by disturbance of the extracellular aminothiol redox state, leading to the development of vascular damage.…”

Section: Discussionmentioning

confidence: 99%

Association of Serum Antioxidant Capacity with Coronary Artery Disease in Middle-aged Men.

Nojiri

Daida

Mikami³

et al. 2001

Jpn Heart J

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SUMMARYThe possible involvement of oxidative damage in the progression of atherosclerosis has been suggested. There is some evidence that antioxidant therapy may be beneficial for the prevention of coronary heart disease. In this study, we investigated the relationship between coronary artery disease (CAD) and serum antioxidative status by measuring the total antioxidant status (TAS). Other relevant antioxidants, such as retinol, α, γ-tocopherol, ascorbic acid, α, β-carotenoids, erythrocyte glutathione peroxidase (GSH-Px) and oxidative products, were also determined in 31 male CAD patients with angiographically defined CAD and 66 male controls, aged 40-70 years, in a case-control study. The TAS levels, ratio and the concentrations of retinol, albumin, total protein and HDL cholesterol were significantly lower in the CAD patients than in the controls (p<0.01), and α-tocopherol andα/γ-tocopherol were significantly higher in the CAD patients than in the controls. The TAS level correlated positively withγ-GTP, GPT, GOT and uric acid (p<0.01). A multiple regression analysis in the CAD patients revealed that the TAS levels correlated most negatively with the number of diseased vessels. The concentrations of carotenoids and GSH-Px, as well as the α/γ-tocopherol ratio were also significantly associated. Although conditional logistic regression analysis suggested low levels of HDL-cholesterol to be a significant coronary risk factor (OR=5.1, 95% CI=1.09-24.3), the TAS level showed no significant independent contribution to CAD. This study demonstrated an association of antioxidant parameters with the atherosclerosis progression, however, it did not confirm antioxidants as an independent risk factor for CAD event. (Jpn Heart J 2001; 42: 677-690)

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Decreased sulfhydryl groups of serum albumin in coronary artery disease.

Cited by 46 publications

References 12 publications

Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus

Are cefuroxime and vancomycin really safe on the corneal endothelial cells?

Association of Serum Antioxidant Capacity with Coronary Artery Disease in Middle-aged Men.

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