Deep imaging for visualizing nitric oxide in lipid droplets: discovering the relationship between nitric oxide and resistance to cancer chemotherapy drugs

Abstract

A near-infrared two-photon fluorescent probe for selective detection and deep-depth imaging of NO helps to discover the relationship between NO and resistance to antitumor drugs.

Lipid droplet evolution gives insight into polyaneuploid cancer cell lipid droplet functions

“…One study demonstrated that DGAT1 in glioblastoma cells is capable of protecting tumors from lipotoxicity, labeling it as a potential metabolic target for cancer therapy [77]. Studies have also shown that the increase of nitric oxide (NO) levels in LDs can protect tumor cells and reduce the efficiency of chemotherapeutic drugs [78].…”

“…Varying studies have been focusing on utilizing NO inhibitors to effectively improve the effect of chemotherapeutic reagents [78]. Additional studies have shown that the chemotherapeutic agents, 5-fluorouracil, and oxaliplatin induce lysophosphatidylcholine acyltransferase 2 (LPCAT2) dependent LD accumulation [79].…”

Three birds with one stone: a single AIEgen for dual-organelle imaging, cell viability evaluation and photodynamic cancer cell ablation

“…Currently, many probes have been developed for the imaging of intracellular active substances and/or single/double organelles. [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] However, because of the p-p stacking and other non-radiative pathways, conventional fluorophores often suffer from an aggregation-caused quenching (ACQ) effect, which might lead to weak fluorescence emission in the aggregation state and easy photobleaching. Fortunately, a new class of aggregation-induced emission (AIE) luminogens that was first proposed by Tang' group in 2001, 30 could perfectly solve the ACQ problems.…”

Observation of macrophage autophagy in the healing of diabetic ulcers via a lysosome-targeting polarity-specific two-photon probe

“…Finally, the deep penetration of near-infrared excitation makes it a good tool for deep tissue imaging. 14 Recent studies have shown that the lysosomal membrane fuses with the autophagosome membrane during the autophagy process, which leads to an increase in its polarity. 15 Therefore, polarity-sensitive fluorescence probes with lysosomal targeting ability are becoming popular autophagy level assessment tools.…”