Defining reference ranges for serum anti-Mullerian hormone (AMH) on a large cohort of normozoospermic adult men highlights new potential physiological functions of AMH on FSH secretion and sperm motility

Benderradji, Hamza; Anne-Laure, Barbotin; Maryse, Leroy-Billiard; Julie, Prasivoravong; Francois, Marcelli; Decanter, Christine; Robin, Geoffroy; Valerie, Mitchell; Jean-Marc, Rigot; Bongiovanni, Antonino; Florent, Sauve; Buée, Luc; Claude-Alain, Maurage; Maryse, Cartigny; Villers, Arnauld; Vincent, Prevot; Sophie, Catteau-Jonard; Sergeant, Nicolas; Paolo, Giacobini; Pigny, P.; Leroy, Clara

doi:10.1530/endoabs.81.p182

Cited by 2 publications

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“…Because serum AMH levels had a significant negative correlation with age (see following discussion), we first compared serum AMH levels between different quartile age groups [Supplementary Table 1, Supplementary Figure 1 ( 27 )]. Adults aged between 39 (third quartile) and 62 (maximum age value) years had significantly lower serum AMH values ( P = 0.02) compared with those between 31 and 35 years old (first and second quartiles, respectively) or under 31 years ( P = 0.02).…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, serum AMH concentrations were negatively correlated with age (r = −0.11; P = 0.009), FSH (r = −0.20; P < 0.0001), and progressive sperm motility (r = −0.10; P = 0.01) and positively correlated with serum inhibin B levels (r = 0.31; P < 0.0001), sperm concentration (r = 0.10; P = 0.01), and total sperm count (r = 0.12; P = 0.004). Also, AMH remained negatively correlated with serum FSH concentrations after adjustment for age and serum inhibin B levels (r = −0.09 ; P = 0.02) [Supplementary Table 2 ( 27 )].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…A significant positive relationship was observed between AMH and serum inhibin B levels after adjustment for age and serum FSH concentrations (r = 0.22; P = 0.0001) [Supplementary Table 3 ( 27 )]. Also, serum AMH concentrations were negatively correlated with progressive sperm motility after adjustment for age and serum FSH or inhibin B concentrations [Supplementary Tables 2 and 3 ( 27 )]. When adjusting for serum FSH levels and age, we observed that the correlations of serum AMH and inhibin B levels with sperm concentration and total sperm count were similar [Supplementary Table 3 ( 27 )].…”

Section: Resultsmentioning

confidence: 99%

“…Also, serum AMH concentrations were negatively correlated with progressive sperm motility after adjustment for age and serum FSH or inhibin B concentrations [Supplementary Tables 2 and 3 ( 27 )]. When adjusting for serum FSH levels and age, we observed that the correlations of serum AMH and inhibin B levels with sperm concentration and total sperm count were similar [Supplementary Table 3 ( 27 )].…”

Section: Resultsmentioning

confidence: 99%

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Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility

Benderradji

Barbotin

Leroy-Billiard

et al. 2022

The Journal of Clinical Endocrinology &Amp; Metabolism

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Background Few studies to date have attempted to measure serum anti-Müllerian hormone (AMH) levels in adult men, and solid references ranges have not yet been defined on a large cohort. Objective In this study, we aimed to first establish the reference ranges for serum AMH and AMH-to-total testosterone ratio (AMH/tT) in adult males. Secondly, we investigated the relationship between serum AMH and both reproductive hormones and semen parameters. Methods This single-center retrospective study included 578 normozoospermic adult men. Serum AMH concentrations were determined with an automated sandwich chemiluminescent immunoassay. Results The median serum AMH was 43.5 pmol/l. The 2.5th and 97.5th percentile values for serum AMH and AMH/tT were 16.4 pmol/l – 90.3 pmol/l, and 0.45 – 3.43, respectively. AMH was positively correlated with inhibin B and sperm concentration, and negatively correlated with age, FSH and progressive sperm motility. Interestingly, using immunofluorescence, we documented for the first time that AMH type-II receptor (AMH-R2) is expressed in ejaculated human spermatozoa and gonadotrophic cells in postmortem pituitary gland. Conclusions A new age-specific reference range for serum AMH and AMH/tT was established. Moreover, AMH-R2 expression in human spermatozoa and gonadotrophic cells, together with the relationship between serum AMH levels and sperm motility or mean FSH levels, highlight new potential functions of AMH in regulating sperm motility or FSH secretion in adult men.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility

Benderradji

Barbotin

Leroy-Billiard

et al. 2022

The Journal of Clinical Endocrinology &Amp; Metabolism

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Low serum anti-Müllerian hormone is associated with semen quality in infertile men and not influenced by vitamin D supplementation

Holt

Yahyavi

Kooij

et al. 2023

BMC Med

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Background Anti-Müllerian hormone (AMH) is released by testicular Sertoli cells and of great importance during fetal male sexual development, but less is known about the role of circulating AMH during adulthood. In vitro studies have shown that vitamin D may induce AMH transcription, but a controlled trial investigating the possible effect of vitamin D on serum AMH has not been conducted in men. Methods A single-center, double-blinded, randomized placebo-controlled clinical trial (NCT01304927) conducted in Copenhagen, Denmark. A total of 307 infertile men were included and randomly assigned (1:1) to a single dose of 300,000 IU cholecalciferol followed by 1400 IU cholecalciferol + 500 mg of calcium daily (n = 151) or placebo (n = 156) for 150 days. Difference in serum AMH was a predefined secondary endpoint. Explorative outcomes were associations between serum AMH and gonadal function in infertile men. The primary endpoint was difference in semen quality and has previously been published. Results Infertile men in the lowest AMH tertile had significantly lower sperm concentration (∆T3-1 16 mill/mL (228%); P < 0.001), sperm count (∆T3-1 55 million (262%); P < 0.001), motile sperm count (∆T3-1 28 million (255%); P < 0.001), progressive motile sperm count (∆T3-1 18 million (300%); P < 0.001), testis size (∆T3-1 2.7 mL (16%); P < 0.001), serum inhibin B (∆T3-1 72 pg/mL (59%); P < 0.001), inhibin B/FSH ratio (∆T3-1 48 (145%); P < 0.001), and higher FSH (∆T3-1 2.6 (38%); P < 0.001) than the tertile of infertile men with highest serum AMH. Vitamin D supplementation had no effect on serum AMH compared with placebo treatment. Conclusions In infertile men, low serum AMH is associated with severely impaired gonadal function illustrated by poor semen quality and lower testosterone/LH ratio. Serum AMH in infertile men was not influenced by vitamin D supplementation.

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Defining reference ranges for serum anti-Mullerian hormone (AMH) on a large cohort of normozoospermic adult men highlights new potential physiological functions of AMH on FSH secretion and sperm motility

Cited by 2 publications

References 0 publications

Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility

Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility

Low serum anti-Müllerian hormone is associated with semen quality in infertile men and not influenced by vitamin D supplementation

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