Definition of new alleles of MIC‐A using sequencing‐based typing

Yao, Zhenjiang; Volgger, A.; Helmberg, Wolfgang; Keller, E.; Fan, Li; Chandanayingyong, D.; Albert, E. D.

doi:10.1046/j.1365-2370.1999.00094.x-i2

Cited by 26 publications

(23 citation statements)

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“…The reaction was performed at the annealing temperature of 65°C. Exons 2, 3, and 4 were sequenced separately using primers described previously (55). For ambiguous allele combinations, 2201-bp MICA PCR product from four donors were cloned using TOPO TA cloning kit for sequencing (Invitrogen Life Technologies) according to the manufacturer's recommendations.…”

Section: Sequencing Of Mica Allelesmentioning

confidence: 99%

NKp46 and NKG2D Recognition of Infected Dendritic Cells Is Necessary for NK Cell Activation in the Human Response to Influenza Infection

Draghi

Pashine

Sanjanwala

et al. 2007

The Journal of Immunology

181

169

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At an early phase of viral infection, contact and cooperation between dendritic cells (DCs) and NK cells activates innate immunity, and also influences recruitment, when needed, of adaptive immunity. Influenza, an adaptable fast-evolving virus, annually causes acute, widespread infections that challenge the innate and adaptive immunity of humanity. In this study, we dissect and define the molecular mechanisms by which influenza-infected, human DCs activate resting, autologous NK cells. Three events in NK cell activation showed different requirements for soluble mediators made by infected DCs and for signals arising from contact with infected DCs. IFN-α was mainly responsible for enhanced NK cytolysis and also important for CD69 up-regulation, whereas IL-12 was necessary for enhancing IFN-γ production. Increased CD69 expression and IFN-γ production, but not increased cytolysis, required recognition of influenza-infected DCs by two NK cell receptors: NKG2D and NKp46. Abs specific for these receptors or their known ligands (UL16-binding proteins 1–3 class I-like molecules for NKG2D and influenza hemagglutinin for NKp46) inhibited CD69 expression and IFN-γ production. Activation of NK cells by influenza-infected DCs and polyinosinic:polycytidylic acid (poly(I:C))-treated DCs was distinguished. Poly(I:C)-treated DCs did not express the UL16-binding protein 3 ligand for NKG2D, and in the absence of the influenza hemagglutinin there was no involvement of NKp46.

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Section: Sequencing Of Mica Allelesmentioning

confidence: 99%

NKp46 and NKG2D Recognition of Infected Dendritic Cells Is Necessary for NK Cell Activation in the Human Response to Influenza Infection

Draghi

Pashine

Sanjanwala

et al. 2007

The Journal of Immunology

181

169

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“…Some of these associations are likely to be secondary associations with alleles of the closely linked HLA‐B locus. For the MICA gene there is evidence for very considerable polymorphism in exons 2–5 with a triplet repeat in exon 5 (14–20). The MICB gene is noticeably less polymorphic than MICA and contains a dinucleotide repeat polymorphism in intron 1 (20–25).…”

mentioning

confidence: 99%

Linkage disequilibria between HLA‐B, C1_4_1, MICA and MICB

Glas¹,

Werner

Brünnler

et al. 2001

Tissue Antigens

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The polymorphisms of MICA exon 5 (5 alleles), MICB intron 1 (13 alleles), C1_4_1 (6 alleles), HLA-B (29 alleles) and HLA-A (15 alleles) were investigated in a healthy German population. Sequencing was performed for the MICB alleles CA14, CA15, CA17, CA23 and CA26 isolated from different cell lines. Variation to the published sequence was observed for CA14, CA15 and for CA17. At the C1_4_1 locus a new allele (CAAA)9 was identified and confirmed by sequencing. Linkage disequilibria were investigated for two-point- and three-point-haplotypes. Although the average relative delta value correlates loosely with the physical distance from HLA-B to MICB: HLA-B-C1_4_1>HLA-B-MICA>HLA-B-MICB, there are several exceptions to this rule. Analyzing three-point-haplotypes for the segment MICB to HLA-A a wide variation of linkage disequilibria for some of the classical HLA-A, B haplotypes has been observed. While the HLA-A1, B8 haplotype displays strong relative delta values over the entire distance from HLA-A to MICB, other haplotypes have linkage disequilibria only in a limited region.

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“…This new primer (5 0 -CAC CTG TGA TTT CCT CTT CCC CAG AGC) corresponds to sequence lying between nucleotide positions 6926 and 6952 in the reference MICA sequence (GI:1405892). The exon 2 forward primers used by others 2,17,18 are also outside the polymorphic region in intron 1.…”

Section: Resultsmentioning

confidence: 99%

“…Methods for genotyping include PCR-SSP, SSOP, RSCA, [10][11][12][13][14][15] along with sequencing-based typing (SBT) usually considered definitive. 2,[16][17][18][19] Results have differed for the same reference cell lines typed by alternative methods. As examples, the cell line WJR076 was typed as *017 by SBT 2 and PCR-SSP, 12 but also reported as *031.…”

Section: Introductionmentioning

confidence: 99%

MICA intron 1 sequences of conserved extended HLA haplotypes: implications for sequencing-based typing

et al. 2004

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The human major histocompatibility complex (MHC) class I chain-related gene A (MICA) has a high degree of genetic diversity. Several methods have been used in MICA typing. Recent studies reported different results for the same reference cell lines typed by different methods. By searching the GenBank, we found an indel polymorphism in MICA intron 1 corresponding to the area where one of the sequencing-based typing primers used by others is located. We investigated this polymorphism in 43 reference samples by primer cycle sequencing. This approach revealed three haplotype-specific patterns of polymorphisms in intron 1. This study provided evidence that one of the primers commonly used in MICA typing may fail to amplify both alleles in certain heterozygous combinations. Our data showed a correlation between the three patterns in MICA intron 1 and exon 5 short tandem repeat (STR) alleles. Being neutral ones, the intron 1 and STR polymorphisms appeared to mark the ancestral lineages better than the coding region polymorphisms.

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Definition of new alleles of MIC‐A using sequencing‐based typing

Cited by 26 publications

References 0 publications

NKp46 and NKG2D Recognition of Infected Dendritic Cells Is Necessary for NK Cell Activation in the Human Response to Influenza Infection

NKp46 and NKG2D Recognition of Infected Dendritic Cells Is Necessary for NK Cell Activation in the Human Response to Influenza Infection

Linkage disequilibria between HLA‐B, C1_4_1, MICA and MICB

MICA intron 1 sequences of conserved extended HLA haplotypes: implications for sequencing-based typing

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