Degradation in the rumen of lupin (Lupinus albus L.) and pea (Pisum sativum L.) seed proteins

Aufrere, Jocelyne; Graviou, Dominique; Melcion, Jean-Pierre; Demarquilly, C.

doi:10.1016/s0377-8401(01)00262-0

Cited by 39 publications

(32 citation statements)

References 47 publications

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“…This observation contrasts with the data of Aufrère et al [2], who observed a decrease in soluble NAN outflow from the rumen after pea extrusion (Baccara variety).…”

Section: Effects Of Extrusioncontrasting

confidence: 99%

“…The extent of this underestimation could be even greater with other varieties of peas such as Baccara or Madria, for which lower rumen N degradability has been reported [2,20]. Furthermore, in the present experiment pea was ground (3-mm screen) and pelleted, generating a small size of particles, which probably enhances the rumen degradability of the pea [4,20].…”

Section: %mentioning

confidence: 60%

“…The in situ rumen N degradability was also decreased but to a lower extent (-10%). With the exception of Petit et al [26], a decrease in rumen pea N degradability in response to extrusion was generally reported [2,32].…”

Section: Effects Of Extrusionmentioning

confidence: 98%

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Rumen digestion and intestinal nutrient flows in sheep consuming pea seeds: the effect of extrusion or chestnut tannin addition

Poncet

Rémond

2002

Anim. Res.

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-Different treatments aimed at reducing rumen degradability of pea protein were evaluated by in situ and in vivo measurements of rumen and intestine digestion of proteins. Four fistulated sheep were used. Pea seed provided 50% of dietary crude protein (CP) and was used raw (RP), with chestnut tannin (RPT2, 20 g·kg -1 of pea CP; RPT3, 30 g·kg -1 of pea CP), and extruded (EP). Rumen in situ degradability of pea protein was decreased by extrusion (83.3% vs. 90.8%) but was not affected by tannin addition. In vivo tannin addition did not affect the organic matter (OM) and N apparent digestibility, at the level of the rumen, the intestine or the whole tract. Extrusion decreased the apparent digestion of OM in the rumen but increased it in the small intestine. Total tract OM digestibility was not affected. Duodenal flow of non-ammonia N (NAN) increased by 27% between the RP and EP diet. This increase was mainly related to an increase in non-microbial N flow. Small intestine NAN apparent digestibility was not affected by extrusion and the amount of NAN apparently digested in the small intestine increased by 23% between the RP and EP diet. A slight decrease in total tract N digestibility was observed with extrusion. The efficiency of microbial protein synthesis was increased by extrusion. Small intestine apparent digestion of amino acids was greater for EP than for RP, but the profile of apparently absorbed amino acids was not affected. This study showed that low doses of tannins (up to 30 g·kg -1 of pea CP, 15 g·kg -1 of dietary CP) were inefficient in decreasing protein rumen degradability, however, the extrusion treatment largely increased the nitrogenous value of the pea seeds. dégradabilité ruminale des protéines du pois a été évalué par des mesures in situ et in vivo de la digestion ruminale et intestinale. Quatre moutons fistulés ont été utilisés. La graine de pois constituait 50 % de la matière azotée totale (MAT) de la ration. Le pois a été distribué cru (RP), avec des tannins de châtaignier (RPT2, 2 % de la MAT du pois ; RPT3, 3 % de la MAT du pois) ou extrudé. La dégra-dabilité théorique du pois a diminué sous l'effet du traitement d'extrusion (83,3 % vs. 90,8 %), mais n'a pas été affectée par l'ajout de tannins. In vivo, l'addition de tannin n'a eu aucun effet sur les paramètres de la digestion de la matière organique (MO) et de l'N. L'extrusion a diminué la digestion apparente de la MO dans le rumen, mais celle-ci a augmenté dans l'intestin grêle. Ainsi la digestibilité de la MO dans l'ensemble du tube digestif n'a pas été modifiée. Le flux duodénal de NAN a augmenté de 27 % entre les régimes RP et EP. Cette augmentation s'explique essentiellement par une augmentation du flux de NAN non-microbien. La digestibilité intestinale apparente n'a pas été modifiée par l'extrusion et la quantité de NAN apparemment digérée dans l'intestin grêle a augmenté de 23 % entre les régimes RP et EP. Une légère diminution de la digestibilité totale de l'N a été observée avec le traitement d'extrusion. L'efficacité de l...

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“…This observation contrasts with the data of Aufrère et al [2], who observed a decrease in soluble NAN outflow from the rumen after pea extrusion (Baccara variety).…”

Section: Effects Of Extrusioncontrasting

confidence: 99%

Section: %mentioning

confidence: 60%

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Rumen digestion and intestinal nutrient flows in sheep consuming pea seeds: the effect of extrusion or chestnut tannin addition

Poncet

Rémond

2002

Anim. Res.

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“…For each cow, the milk sample (100 mL) from the evening milking was cooled (4 °C) and mixed (1/1) with milk collected the next morning. A few mL were frozen (-18 °C) for urea [36,37]: 4 Net Energy, 5 Digestible protein in the small intestine, 6 Degradable N / Fermentable energy balance in the rumen.…”

Section: Methodsmentioning

confidence: 99%

Suitability of lupin and pea seeds as a substitute for soybean meal in high-producing dairy cow feed

Froidmont

Bartiaux-Thill

2004

Anim. Res.

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-Two experiments were conducted to investigate the substitution of soybean meal by coarsely ground lupin and/or pea seeds in high-producing dairy cow feed. In experiment 1 (Exp. 1), four Holstein dairy cows (35.9 ± 2.2 L·d -1 milk) were put on a control diet consisting of 50% maize silage, 11% grass silage and 36% concentrates on a dry-matter (DM) basis. Soybean meal was partially replaced (75%) by lupin, pea and a mixture (1/1, DM) of lupin and pea on a DM basis, following a 4 × 4 Latin square design. Milk production was lower with pea seeds, intermediate with a lupin/ pea seed mixture and higher with lupin and soybean meal diets. Milk fat percentage increased with the lupin diet, which induced a lower proportion of medium-chain fatty acids and a higher proportion of long-chain fatty acids in the milk compared to the pea diet. In experiment 2 (Exp. 2), six Holstein dairy cows (36.0 ± 4.9 L·d -1 milk) were put on a control diet consisting of 47% maize silage, 7% grass silage and 42% concentrates on a DM basis. Soybean meal was totally replaced by lupin or a lupin/pea mixture (1/1, N) on a nitrogen basis, following a duplicated Latin square design (6 animals × 3 diets). Standard milk production did not differ with the dietary protein source but milk fat percentage was reduced with the lupin diet. This effect, not observed in Exp. 1, was probably related to the lipid content of the lupin diet. Nitrogen efficiency was not modified by the protein source. The results also showed that a high ingestion of lupin seeds induced a lower milk ω6/ω3 ratio and C 18:2 content. In conclusion, coarsely ground lupin seeds can efficiently replace soybean meal in high-producing dairy cow feed. dairy cow / soybean meal / lupin / pea / milk Résumé -Les graines de lupin et de pois en tant qu'alternative au tourteau de soja dans l'alimentation des vaches laitières hautes productrices. Deux expériences ont été menées pour étudier la substitution du tourteau de soja par du lupin et/ou du pois grossièrement moulu dans l'alimentation de la vache laitière haute productrice. Dans l'expérience 1, quatre vaches laitières Holstein (35,9 ± 2,2 L·j -1 de lait) ont reçu un régime témoin contenant 50 % d'ensilage de maïs, 11 % d'ensilage d'herbe et 36 % de concentré sur base de la matière sèche (MS). Le tourteau de soja a été partiellement remplacé (75 %) par du lupin, du pois et un mélange (1/1, MS) de lupin et de pois sur base de la MS, selon un dispositif expérimental en carré latin 4 × 4. La production laitière a été inférieure avec le pois, intermédiaire avec le mélange lupin/pois et supérieure avec les régimes lupin * Corresponding author: froidmont@cra.wallonie.be 476 E. Froidmont, N. Bartiaux-Thill et tourteau de soja. Le taux butyreux du lait a augmenté avec le régime lupin, qui a induit une plus faible proportion d'acides gras à chaîne moyenne et une plus grande proportion d'acides gras à longue chaîne comparativement au régime pois. Dans l'expérience 2, six vaches laitière Holstein (36,0 ± 4,9 L·j -1 de lait) ont reçu un régime ...

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“…Due to their high protein content, the European Union has promoted the production of peas (Pisum sativum; Aufrere et al, 2001) although its proteins have the limitation to be also highly degradable in the rumen (INRA, 2007;Khorasani et al, 2001;Petit et al, 1997). Therefore, to reduce this degradability without reducing the intestinal digestibility of the by-pass protein is of interest to improve the use of these both meals.…”

Section: Introductionmentioning

confidence: 99%

Potenciación de la respuesta productiva en rumiantes mediante el empleo de proteínas protegidas

Royón¹

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SUMMARY XIThe main objective of this thesis project was to increase the protein efficiency in ruminant diets by using protected protein (sunflower meal and spring pea), and improving the prediction of microbial protein supply.Commercial sunflower meal (SFM) and spring pea (SP) were treated with 4 N solutions (200 mL/kg) of malic acid (268.2 g/L) or orthophosphoric acid (130.6 g/L). Daily, two fractions of 12.5 kg of one of these meals were successively sprayed with the tested acid solution in a concrete mixer using a sprayer. Both fractions were then mixed and allowed to rest for 1 h at room temperature. The blend was then dried in a forced air oven at 120 ºC for 1 h. Then the oven was turned off and the treated material was left in the oven overnight. During the drying process, the material was stirred every 30 min during the first 2 h and then every 60 min for the subsequent 5 h. This process was repeated until the amounts of treated flour needed for the different trials performed.In the first experiment (chapter 3), ruminal and intestinal digestion trials were conducted to study the effects of the application of these acid solutions and heat to protect proteins of SFM and SP against ruminal degradation using three wethers fitted with rumen and duodenum cannulae. The ruminal digestion study was carried out in three experimental periods in which the wethers were successively fed three isoproteic diets including SFM and SP, untreated or treated with malic or orthophosphoric acids. The experimental periods of 21 days included successively: 10 days of diet adaptation, SFM and SP particle ruminal transit study (days 11-14) and ruminal nylon-bag incubations (days 15-21). The meals incubated in each experimental period were those corresponding to the associated diet. Rumen bacteria were labelled from days 11 to 21 by continuous intra-ruminal infusion of a 15 N source and the rumen was emptied at the end of in situ incubations in each period to isolate solid adherent bacteria and liquid associate bacteria. The intestinal digestion trial was conducted twenty days after the end of the ruminal studies to eliminate the 15 N enrichment in the digesta. The tested samples XII were composite samples obtained pooling the different ruminally undegraded residues to be representative of the chemical composition of the ruminally undegraded fraction (RU).Wethers were fed the untreated diet to determine the intestinal digestibility of untreated and treated meals using the mobile nylon bag technique. In addition, protein in untreated and treated meals and their 0 h, composite and intestinally undigested samples were extracted and subjected to electrophoresis to determine the digestion site of the different protein fractions.Estimates of the RU and its intestinal digestibility of dry matter, organic matter (only for RU), crude protein (CP) and starch (only in SP) were obtained considering ruminal microbial contamination and particle comminution and outflow rates. When corrected for the microbial contamination taking place in the rume...

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Degradation in the rumen of lupin (Lupinus albus L.) and pea (Pisum sativum L.) seed proteins

Cited by 39 publications

References 47 publications

Rumen digestion and intestinal nutrient flows in sheep consuming pea seeds: the effect of extrusion or chestnut tannin addition

Rumen digestion and intestinal nutrient flows in sheep consuming pea seeds: the effect of extrusion or chestnut tannin addition

Suitability of lupin and pea seeds as a substitute for soybean meal in high-producing dairy cow feed

Potenciación de la respuesta productiva en rumiantes mediante el empleo de proteínas protegidas

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