Degradation of Fumonisin B1 by a Bacterial Strain Isolated from Soil

Benedetti, R; Nazzi, Francesco; Locci, R.; Firrao, Giuseppe

doi:10.1007/s10532-005-2797-y

Cited by 38 publications

(28 citation statements)

References 22 publications

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“…Previous research identified degradation of FB1 by microorganisms is likely composed of two essential steps: hydrolysis to hydrolyzed fumonisin B1 (HFB1) and deamination of the hydrolysis product (Heinl et al, 2011). Benedetti et al (Benedetti et al, 2006) demonstrated that different bacterial isolates of maize ecosystem have the ability to degrade FB1, among of which, Sphingomonas sp. MTA144 (Meca et al, 2013), could encode aminotransferase FBI, showing strong fumonisin degrading activity .…”

Section: Discussionmentioning

confidence: 99%

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Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Fu¹,

Feng²,

YaNan³

et al. 2015

IJAB

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Corn ear rot has been the most important disease of the world. It is mainly caused by Fusarium moniliforme which produce a number of mycotoxins including Fumonisin B1 (FB1). Aiming at supplying a potentially effective biological control agent against F. moniliforme, Bacillus megaterium MHT6 isolated from China, was tested for its antagonistic activity against F. moniliforme. Moreover, both antifungal activity and the active compound of strain MHT6 were tested and identified. In order to improve its antifungal activity, a random mutagenesis generated using He-Ne laser irradiation was applied on B. megaterium MHT6. After the mutation, one hundred bacterial colonies were obtained and their effects against F. moniliforme were assessed in a plate assay. Eight colonies (8%) exhibited higher inhibition activity as compared to the wild type MHT6. Among them, the mutant MHT 88 which showed the strongest antagonistic ability was selected out. Through bacterial motility assays, the mutant MHT88 showed stronger swarming motility than wild type MHT6. According to FPLC and MALDI-TOF-MS, the production of two main antagonistic lipopeptides such as surfactin and fengycin increased in mutant MHT88. Especially the surfactin, which can't be detected in the wild strain MHT6, was obviously observed in mutant. Moreover, the cell-free supernatant of the mutant MHT88 showed stronger capability in degrading FB1 compared to MHT6. This study provided a promising biological agent to inhibit F. moniliforme and suppress corn ear rot disease.

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Section: Discussionmentioning

confidence: 99%

“…According to previous report (Benedetti et al, 2006), Fumonisin B1 (FB1) could be degraded by a Bacillus sp. strain.…”

Section: Degradation Of Fb1 In Medium By Mutant Mht88 and Wild Strainmentioning

confidence: 94%

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Fu¹,

Feng²,

YaNan³

et al. 2015

IJAB

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“…In previous studies several microorganisms capable of degrading fumonisin B 1 have been identified (Benedetti et al 2006;Blackwell et al 1999;Duvick et al 1998;Täubel 2005). Biodegradation of fumonisin B 1 in the black yeast Exophiala spinifera was shown to comprise two main steps: hydrolysis of FB 1 to HFB 1 by a carboxylesterase (Duvick et al 1998) and deamination of HFB 1 by an amino oxidase (Blackwell et al 1999).…”

Section: Introductionmentioning

confidence: 98%

An aminotransferase from bacterium ATCC 55552 deaminates hydrolyzed fumonisin B1

Heinl

Hartinger²,

Thamhesl

et al. 2010

Biodegradation

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Previous research identified several microorganisms and pathways capable of degrading the mycotoxin fumonisin B₁ (FB₁). Degradation of FB₁ by microorganisms seems to comprise two essential steps: hydrolysis to hydrolyzed fumonisin B₁ (HFB₁) and deamination of the hydrolysis product. One of the previously studied microorganisms was the Gram negative bacterium ATCC 55552. The gene corresponding to the first step of FB₁ degradation in this bacterium was identified, but the genetic basis for deamination of the hydrolyzed intermediate remained unexplained (Duvick et al. 2000, PCT patent application WO200004158). Here we report the sequence and HFB₁-deaminating activity of a novel aminotransferase encoded by the bacterium ATCC 55552. The corresponding gene was identified, sequenced, and over-expressed in Escherichia coli. Cell lysates of the recombinant E. coli strain showed distinct HFB₁-deaminating activity in the presence of pyridoxal phosphate and pyruvate, as was demonstrated by liquid chromatography-mass spectrometry. Thus, we suggest the novel enzyme to be part of the fumonisin catabolic pathway of the bacterium ATCC 55552.

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“…The few TLC methods had been used for screening and not for quantification (Abbas et al, 2004;Benedetti et al, 2006). GC with highresolution MS had also been used for analysis of some mycotoxins (Fernandez et al, 2007;Ikonomou et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…However, a labour-intensive derivatization step was often indispensable prior to GC analysis. Therefore, HPLC-MS-MS had increasingly become the method of choice for mycotoxin analysis (Abbas et al, 2004;Benedetti et al, 2006;Fernandez et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Mycotoxins in animals: Occurrence, effects, prevention and management

Zaki¹

2012

J. Toxicol. Environ. Health Sci.

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Globalization of the trade in agricultural commodities has contributed significantly to the discussion about potential hazards involved and has increased in particular the awareness of mycotoxins. Safety awareness in food and feed production has also risen due to the simple fact that methods for testing residues and undesirable substances have become noticeably more sophisticated and more available at all points of the supply chain. Mycotoxins comprise of a family of fungal toxins, many of which have been implicated as chemical progenitors of toxicity in man and animals. There are four classes of mycotoxins of major concern namely aflatoxins, zearalenone, ochratoxins, and fumonisins. Formation of mycotoxins varied between species as well as within a given species. A variety of physical, chemical, and biological methods to counteract the mycotoxin problem have been reported, but large-scale, practical, and cost-effective methods for detoxifying mycotoxin-containing feedstuffs are currently not available. Detoxification strategies for the contaminated foods and feeds should be done to reduce or eliminate the adverse actions of mycotoxin to improve food safety and prevent economic losses. The most recent approach to the problem has been the addition to the animal's diet of nonnutritive sorbents that sequester mycotoxins, reduce their gastrointestinal absorption and avoiding their toxic effects on livestock and toxin carryover into animal products. This review comments on the potential hazards of several mycotoxins together with prevention strategy for fungal and mycotoxin contamination.

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Degradation of Fumonisin B1 by a Bacterial Strain Isolated from Soil

Cited by 38 publications

References 22 publications

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

An aminotransferase from bacterium ATCC 55552 deaminates hydrolyzed fumonisin B1

Mycotoxins in animals: Occurrence, effects, prevention and management

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