Degradation of Perfluorooctane Sulfonamide by <i>Acinetobacter</i> Sp. M and Its Extracellular Enzymes

Hao, Jian; Wang, Penghong; Kang, Yufei; He, Hui; Luo, Huihua; Kim, Sarah; Niu, Lili; Jiang, Haizhen; Ma, Kesen

doi:10.1002/asia.201900638

Cited by 9 publications

(3 citation statements)

References 35 publications

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“…LUNF3 enriches the resources of PAE-degrading bacteria. The genus Acinetobacter can also eliminate other pollutants, such as sulfamethoxazole [ 32 ], perfluorooctane sulfonamide [ 33 ], crude oil [ 34 ] and phenol [ 35 ]. Therefore, they have a potential application in the remediation of contaminated environments.…”

Section: Resultsmentioning

confidence: 99%

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism

Fan,

Guo,

Han

et al. 2023

Molecules

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Phthalic acid esters (PAEs), which are widespread environmental contaminants, can be efficiently biodegraded, mediated by enzymes such as hydrolases. Despite great advances in the characterization of PAE hydrolases, which are the most important enzymes in the process of PAE degradation, their molecular catalytic mechanism has rarely been systematically investigated. Acinetobacter sp. LUNF3, which was isolated from contaminated soil in this study, demonstrated excellent PAE degradation at 30 °C and pH 5.0–11.0. After sequencing and annotating the complete genome, the gene dphAN1, encoding a novel putative PAE hydrolase, was identified with the conserved motifs catalytic triad (Ser201-Asp295-His325) and oxyanion hole (H127GGG130). DphAN1 can hydrolyze DEP (diethyl phthalate), DBP (dibutyl phthalate) and BBP (benzyl butyl phthalate). The high activity of DphAN1 was observed under a wide range of temperature (10–40 °C) and pH (6.0–9.0). Moreover, the metal ions (Fe2+, Mn2+, Cr2+ and Fe3+) and surfactant TritonX-100 significantly activated DphAN1, indicating a high adaptability and tolerance of DphAN1 to these chemicals. Molecular docking revealed the catalytic triad, oxyanion hole and other residues involved in binding DBP. The mutation of these residues reduced the activity of DphAN1, confirming their interaction with DBP. These results shed light on the catalytic mechanism of DphAN1 and may contribute to protein structural modification to improve catalytic efficiency in environment remediation.

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Section: Resultsmentioning

confidence: 99%

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism

Fan,

Guo,

Han

et al. 2023

Molecules

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“…Acinetobacter is a common bacteria in soil. It has been found that Acinetobacter can degrade the sulfur and phosphorus of pesticides, which can repair soil polluted by pesticides, and some species have degradation functions [ 56 ]. In our study, we found that the abundance of Acinetobacter species decreased after AITC fumigation, which may be related to their short life cycles [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Allyl Isothiocyanate on the Soil Microbial Community Structure and Composition during Pepper Cultivation

Gao

Pei

Xie

2021

J. Microbiol. Biotechnol.

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Allyl isothiocyanate (AITC), as a fumigant, plays an important role in soil control of nematodes, soilborne pathogens, and weeds, but its effects on soil microorganisms are unclear. In this study, the effects of AITC on microbial diversity and community composition of Capsicum annuum L. soil were investigated through Illumina high-throughput sequencing. The results showed that microbial diversity and community structure were significantly influenced by AITC. AITC reduced the diversity of soil bacteria, stimulated the diversity of the soil fungal community, and significantly changed the structure of fungal community. AITC decreased the relative abundance of dominant bacteria Planctomycetes, Acinetobacter, Pseudodeganella, and RB41, but increased that of Lysobacter, Sphingomonas, Pseudomonas, Luteimonas, Pseudoxanthomonas, and Bacillus at the genera level, while for fungi, Trichoderma, Neurospora, and Lasiodiplodia decreased significantly and Aspergillus, Cladosporium, Fusarium, Penicillium, and Saccharomyces were higher than the control. The correlation analysis suggested cellulase had a significant correlation with fungal operational taxonomic units and there was a significant correlation between cellulase and fungal diversity, while catalase, cellulose, sucrase, and urease were the major contributors in the shift of the community structure. Our results will provide useful information for the use of AITC in the assessment of environmental and ecological security.

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“…In different soil layers, with the application of atrazine, the abundance of Nitrospira , Pseudomonas , Sulfuricurvum , Acinetobacter , Flavobacterium , Salinibacterium , and Pedobacter in AT groups increased significantly compared with CK groups. These bacteria play an important role in the degradation of pesticides [ 43 ], heavy metals [ 44 ], lignin [ 45 ], perfluorooctane sulfonic acid [ 46 ], polycyclic aromatic hydrocarbons [ 47 ], polychlorinated biphenyls [ 48 ], and cellulose [ 49 ], which may be the reason for the increase in abundance of these bacteria after atrazine application for a period of time.…”

Section: Discussionmentioning

confidence: 99%

Effects of Atrazine on Chernozem Microbial Communities Evaluated by Traditional Detection and Modern Sequencing Technology

Fang

Gao

et al. 2021

Microorganisms

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Atrazine is a long residual herbicide commonly used in maize fields. Although atrazine can effectively control weeds and improve crop yield, long-term application leads to continuous pollution in the agricultural ecological environment, especially in the soil ecosystem, and its impact on soil microorganisms is still not clear. Four methods were used in the experiment to clarify the effect of atrazine on the bacterial populations of cultivated soil layers of chernozem in a cold region in different periods: high-performance liquid chromatography (HPLC), colorimetry, microplate, and high-throughput sequencing. The level of residual atrazine in cold chernozem decreased from 4.645 to 0.077 mg/kg soil over time, and the residue gradually leached into deep soil and then decreased after accumulating to a maximum value. Atrazine significantly affected the activities of urease and polyphenol oxidase activity in the soil layers at different periods but had no significant effect on sucrase and phosphatase activity. Atrazine significantly reduced the diversity of microbial carbon source utilization and total activity in soil layers of 0–10 and 20–30 cm but only reduced the diversity of microbial carbon source utilization in the 10–20 cm layer. Atrazine had no significant effect on bacterial populations (10–12 phyla, 29–34 genera), but had a slight effect on the relative abundance of various groups. Atrazine significantly reduced the diversity of bacterial populations in cultivated soil layers of chernozem in a cold region, and the diversity of bacterial populations decreased with decreased residue. This lays a foundation for guiding the safe use of herbicides on farmland in Northeast China.

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Degradation of Perfluorooctane Sulfonamide by Acinetobacter Sp. M and Its Extracellular Enzymes

Cited by 9 publications

References 35 publications

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism

A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism

Influence of Allyl Isothiocyanate on the Soil Microbial Community Structure and Composition during Pepper Cultivation

Effects of Atrazine on Chernozem Microbial Communities Evaluated by Traditional Detection and Modern Sequencing Technology

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