Degradation of polyesters by a novel marine Nocardiopsis aegyptia sp. nov.: Application of Plackett-Burman experimental design for the improvement of PHB depolymerase activity

Ghanem, Nagia M.; Mabrouk, Mona E. M.; Sabry, Soraya A.; El-Badan, Dalia E.

doi:10.2323/jgam.51.151

Cited by 48 publications

(24 citation statements)

References 40 publications

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“…Growth for 7 days was the optimum incubation period for PHB degradation in agar medium containing 2% PHB as carbon source. This result differed with that obtained by Hsu et al (2012) who isolated Streptomyces bangladeshensis 77T-4 from soil and observed maximum PHB degradation on plates after 4 days while Ghanem et al (2005) found that the maximum degradation activity was observed in test tubes after 21 days but the maximum activity in plates after 25 days. PHB degradation in plates was faster than test tubes.…”

Section: Discussioncontrasting

confidence: 98%

“…Many factors influencing on PHB degradation including pH, incubation temperature and incubation period by Aspergillus fumigates in plates were studied. Ghanem et al (2005) used the Mineral PHB degradation medium with plates to determine the degradation activity by Nocardiopsis aegyptia which isolated from marine sediment. Lee et al (2005) used 0.1 to 0.02% PHB concentrations with agar plates to more easily visualize clear zones.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia

Aly¹,

Jaar²,

Bokhari³

2017

IOSRJPBS

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia

Aly¹,

Jaar²,

Bokhari³

2017

IOSRJPBS

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“…To the time of writing, bioplastic degradation has been reported for only three halotolerant and halophilic actinobacteria genera including Stretoalloteichus hindustanus JCM 3268; degrading poly(LAs) (Jarerat et al 2002 ), Streptomyces and Nocardiopsis aegyptia (Ghanem et al 2005 ), and obligate marine Streptomyces sp. SNG9 (Mabrouk and Sabry 2001 ).…”

Section: Polyester and Bioplastic Degradationmentioning

confidence: 99%

Biotechnological Exploitation of Actinobacterial Members

Hamedi

Mohammadipanah

Panahi

2015

Sustainable Development and Biodiversity

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Microbial-derived products, while do not have many environmental side effects of their synthetic counterparts, have proved more effi cient than those synthetically obtained. The nature has provided a treasure of microorganisms with capabilities to produce vast variety of novel compounds. This ability has been arised during long last of evolution and adaptation to diverse chemical and physical microenviroments. Microbial-derived metabolites have made their own space in industries and therefore human life. Among these microorganisms, halophilic and halotolerant actinobacteria are recently gaining much attentions. Metabolites and biological functions from halophilic or halotolerant members of this phylum of bacteria may resolving the ever-increasing thirst of industry for metabolites with salttolerancy to cope a range of issues from environmental pollution to diseases and world's hunger. In the current chapter, it has been tried to introduce the less dealt group of halophilic and halotolerant actinobacteria, and shed light on their potential to be exploited in various industry sectors.

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“…In order to optimize enzyme production, the Plackett-Burman design was applied, which has been demonstrated as an efficient approach to screen for medium components and/or factors affecting PHB degradation. According to Ghanem et al (2005) increasing PHB level negatively affect the enzyme activity which led to suggest that the PHB depolymerase is constitutive. Hydrolytic activity of PHB depolymerase is strongly enhanced in presence of low concentrations of divalent cations such as calcium, magnesium or zinc.…”

Section: Evaluating the Significance Of Medium Constituents Using Thementioning

confidence: 99%

Degradation of Poly-β-hydroxybutyrate BY Trichoderma asperellum

Alhazmi¹,

Ghanem²,

Ahwany³

et al. 2018

JEBAS

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In this work, sequential optimization strategy, based on statistical designs, was employed to enhance the degradation of PHB by Trichoderma asperellum through the measurement of PHB depolymerase specific activity. For PHB degradation screening, two-level Plackett-Burman design was used. Result of study revealed that fermentation medium composition significantly influencing the PHB depolymerase specific activity. Further, it was reported that time of incubation, levels of PHB and concentration of glucose were found to be the major factors influencing the enzyme specific activity. A second threelevel Box-Behnken design was applied to acquire the best process conditions for PHB depolymerase specific activity. In this study, the initial basal medium showed a PHB depolymerase specific activity of 40 ng / l / hr / g dry wt and through the two successive statistical design, the final level of PHB depolymerase specific activity was 3230 ng / l / hr / g drywt, this means a nearly of 80 fold increase.

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Degradation of polyesters by a novel marine Nocardiopsis aegyptia sp. nov.: Application of Plackett-Burman experimental design for the improvement of PHB depolymerase activity

Cited by 48 publications

References 40 publications

Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia

Poly-𝛽-hydroxybutyrate degradation by Aspergillus fumigates isolated from soil samples collected from Jeddah, Saudi arabia

Biotechnological Exploitation of Actinobacterial Members

Degradation of Poly-β-hydroxybutyrate BY Trichoderma asperellum

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