Degradation of the resin–dentin bonds after simulated and inhibited cariogenic challenge in an <i>in situ</i> model

Reinke, Stella Maria Glaci; Lawder, Juliana Aparecida de Campos; Divardin, Sindianara; Raggio, Daniela Prócida; Reis, Alessandra; Loguércio, Alessandro Dourado

doi:10.1002/jbm.b.32708

Cited by 12 publications

(15 citation statements)

References 41 publications

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“…Moreover, the incorporation of PAMAM and DMADDM regulated the bacteria ratio of the three-species biofilms and help to develop a biofilm with lower cariogenic S. mutans proportion and reduced possibility of dental caries. Previous studies showed that it was favorable for adhesive to inhibit or kill residual bacteria in the prepared tooth cavity, as well as invading bacteria at the tooth-restoration interface through marginal leakage [18,19]. In the present study, the adhesive containing 5% DMADDM and 1% PAMAM + 5% DMADDM had strong antibacterial effect, which was consistent with previous studies [20,21].…”

Section: Discussionsupporting

confidence: 91%

Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

et al. 2017

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The mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications.

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Section: Discussionsupporting

confidence: 91%

Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

et al. 2017

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“…the micro-tensile test and the shear test [16, 23]. Because the specimens are small in the micro-tensile test, extensive machining is required which can lead to large amount of premature failure and significant variation in bond strength measurement.…”

Section: Discussionmentioning

confidence: 99%

Degradation in the dentin–composite interface subjected to multi-species biofilm challenges

Li¹,

Carrera²,

Chen³

et al. 2014

Acta Biomaterialia

114

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Oral biofilms can degrade the components in dental resin-based composite restorations, thus compromising marginal integrity and leading to secondary caries. In this study, we investigated the mechanical integrity of the dentin-composite interface challenged with multi-species oral biofilms. While most studies used single-species biofilms, we used a more realistic, diverse biofilm model produced directly from plaques collected from donors with a history of early childhood caries. Dentin–composite disks were made using bovine incisor roots filled with Z100™ or Filtek™ LS (3M ESPE). The disks were incubated for 72hr in paired CDC biofilm reactors, using a previously published protocol. One reactor was pulsed with sucrose, and the other was not. A sterile saliva-only control group was run with sucrose pulsing. The disks were fractured under diametral compression to evaluate their interfacial bond strength. Surface deformation of the disks was mapped using digital image correlation (DIC) to ascertain fracture origin. Fracture surfaces were examined using SEM/EDS to assess demineralization and interfacial degradation. Dentin demineralization was greater under sucrose-pulsed biofilms, as the pH dropped below 5.5 during pulsing, with LS and Z100 specimens suffering similar degrees of surface mineral loss. Biofilm growth with sucrose pulsing also caused preferential degradation of the composite-dentin interface, depending on the composite/adhesive system used. Specifically, Z100 specimens showed greater bond strength reduction and more frequent cohesive failure in the adhesive layer. This was attributed to the inferior dentin coverage by Z100 adhesive which possibly led to a higher level of chemical and enzymatic degradation. The results suggested that factors other than dentin demineralization were also responsible for interfacial degradation. We have thus developed a clinically relevant in vitro biofilm model which would allow us to effectively assess the degradation of the dentin-composite interface subjected to multi-species biofilm challenge.

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“…One of these processes involves the pH fluctuations caused by cariogenic challenges, which can reduce bond strength. 23 In addition, in situ models are appropriate for evaluating bond degradation because host-derived MMPs can originate from saliva and from dentin. 24 Recently, Reinke and others 23 reported that the in situ model seems to be a suitable short-term methodology to investigate the degradation of resin-dentin bonds under a more realistic condition.…”

Section: Introductionmentioning

confidence: 99%

Influence of Chlorhexidine and/or Ethanol Treatment on Bond Strength of an Etch-and-rinse Adhesive to Dentin: An In Vitro and In Situ Study

Simões

Basting

Amaral

et al. 2014

Operative Dentistry

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Bond strength suffered degradation over time and was not influenced by dentin treatments with chlorhexidine and/or ethanol. Adhesive bond degradation was less affected under in situ conditions than in vitro. SUMMARYThe aim of this study was to evaluate the effect of a chlorhexidine and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system either under in vitro aging or in situ cariogenic challenge. The dentin surface of 36 human third molars were flattened and allocated into four groups to be treated with chlorhexidine, ethanol, or chlorhexidine + ethanol or left unexposed to any solution (control) (n=9). Then, a resin composite restoration was made on the dentin surface and longitudinal sticks were obtained. Sticks from each tooth were assigned to three test conditions: stored in water in vitro for 24 hours, stored in water in vitro for 6 months, or worn in situ for 14 days. During in situ wear time, a high-cariogenic challenge condition was simulated. Specimens were tested for microtensile bond strength (lTBS). Multivariate analysis of variance and Tukey's test showed that chlorhexidine, ethanol, or chlorhexidine + ethanol did not affect the lTBS. The in vitro lTBS values were significantly lower for the specimens stored for 6 months than for those stored for 24 hours. Intermediate lTBS values were shown by the specimens worn in situ. Thus, use of chlorhexidine and/or ethanol was incapable of containing the degradation , 2014, 39-1, 64-71 at the bond interface in the in vitro model. The in situ model was capable of reducing bond strength similarly to the in vitro/6 months model. Despite this, the in situ bond strength was still similar to that of the in vitro/24-hour model.

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Degradation of the resin–dentin bonds after simulated and inhibited cariogenic challenge in an in situ model

Cited by 12 publications

References 41 publications

Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

Novel Dental Adhesive with Biofilm-Regulating and Remineralization Capabilities

Degradation in the dentin–composite interface subjected to multi-species biofilm challenges

Influence of Chlorhexidine and/or Ethanol Treatment on Bond Strength of an Etch-and-rinse Adhesive to Dentin: An In Vitro and In Situ Study

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