Influence of Chlorhexidine and/or Ethanol Treatment on Bond Strength of an Etch-and-rinse Adhesive to Dentin: An In Vitro and In Situ Study

Abstract

Bond strength suffered degradation over time and was not influenced by dentin treatments with chlorhexidine and/or ethanol. Adhesive bond degradation was less affected under in situ conditions than in vitro. SUMMARYThe aim of this study was to evaluate the effect of a chlorhexidine and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system either under in vitro aging or in situ cariogenic challenge. The dentin surface of 36 human third molars were flattened and allocated into four groups to be treated with chlorhexidine, ethanol, or chlorhexidine + ethanol or left unexposed to any solution (control) (n=9). Then, a resin composite restoration was made on the dentin surface and longitudinal sticks were obtained. Sticks from each tooth were assigned to three test conditions: stored in water in vitro for 24 hours, stored in water in vitro for 6 months, or worn in situ for 14 days. During in situ wear time, a high-cariogenic challenge condition was simulated. Specimens were tested for microtensile bond strength (lTBS). Multivariate analysis of variance and Tukey's test showed that chlorhexidine, ethanol, or chlorhexidine + ethanol did not affect the lTBS. The in vitro lTBS values were significantly lower for the specimens stored for 6 months than for those stored for 24 hours. Intermediate lTBS values were shown by the specimens worn in situ. Thus, use of chlorhexidine and/or ethanol was incapable of containing the degradation , 2014, 39-1, 64-71 at the bond interface in the in vitro model. The in situ model was capable of reducing bond strength similarly to the in vitro/6 months model. Despite this, the in situ bond strength was still similar to that of the in vitro/24-hour model.

Effect of 2% Chlorhexidine Following Acid Etching on Microtensile Bond Strength of Resin Restorations: A Meta-Analysis

“…In the 24 h subgroup meta-analysis, as in the immediate follow-up group, chlorhexidine application did not influence microtensile bond strength. This is in agreement with the findings of several authors [24][25][26][27][28]30,31].…”

Effect of digluconate chlorhexidine on bond strength between dental adhesive systems and dentin: A systematic review

“…[ 35 ] found that the incorporation of 2% CHX into ethanol-wet bonding preserved bond strength to both sound and caries-affected dentin, and reduced interfacial nanoleakage after 12 months, while the incorporation of 2% CHX to water-wet bonding preserved bond strength only to sound dentin after aging. Nevertheless, Simoes et al .,[ 37 ] who evaluated the effect of CHX and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system under either in vitro aging or in situ cariogenic challenge, showed that CHX, ethanol, or CHX + ethanol did not affect the μTBS after 6 months.…”

Effect of Galardin and its Solvents on the Microtensile Bond Strength of Different Adhesive Systems to Dentin

“…In addition, it was showed that use of absolute alcohol resulted in a non-significant decrease in µTBS compared to the control group [22]. Some authors evaluated the effect of ethanol and chlorhexidine (CHX) on the bond strength and reported that both solvents resulted in a non-significant decrease in bond strength after 24 hours, with greater decrease in bond strength with the use of ethanol compared to CHX [23].…”