Degradation of Total Petroleum Hydrocarbon (TPH) in Contaminated Soil Using &lt;i&gt;Bacillus pumilus&lt;/i&gt; MVSV3

Varma, Surendra Sheeba; Lakshmi, Mahalingam Brinda; Rajagopal, Perumalsam; Velan, M.

doi:10.4265/bio.22.17

Cited by 17 publications

(6 citation statements)

References 22 publications

(20 reference statements)

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“…are more tolerant to high concentrations of polycyclic aromatic hydrocarbons in soil due to their resistant endospores, so the isolates belonging to the Bacillus sp. could be effective in the removal of PAHs in the contaminated soils [2,[38][39][40][41]. e 34 Bacillus isolates in this study are able to grow in 24 hours or 48 hours on BH medium supplemented with different carbon sources (gasoline, diesel oil, benzene, hexane and olive oil) 2% (v/v).…”

Section: Discussionmentioning

confidence: 90%

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Profiling of Indigenous Biosurfactant-Producing Bacillus Isolates in the Bioremediation of Soil Contaminated by Petroleum Products and Olive Oil

Elenga-Wilson

Kayath

Mokemiabeka

et al. 2021

International Journal of Microbiology

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Petroleum is, up to this date, an inimitable nonrenewable energy resource. Petroleum leakage, which arises during transport, storage, and refining, is the most important contaminant in the environment, as it produces harm to the surrounding ecosystem. Bioremediation is an efficient method used to treat petroleum hydrocarbon-contaminated soil using indigenous microorganisms. The degradation characteristics for a variety of hydrocarbons (hexane, benzene, gasoline, and diesel) were qualitatively and quantitatively investigated using Bacillus isolates. Microbiological and biochemical methods have been used including isolation of oil-degrading bacteria, enzymatic activities, the determination of physicochemical parameters, biosurfactant production and extraction assay, oil displacement assay, antimicrobial assay of the biosurfactants, and bioremediation kinetics. Consequently, of the 60 isolates capable of degrading different hydrocarbons at fast rates, 34 were suspected to be Bacillus isolates capable of growing in 24 h or 48 h on BH medium supplemented with 2% of hexane, benzene, gasoline, diesel, and olive oil, respectively. Among the 34 isolates, 61% (21/34) are capable of producing biosurfactant-like molecules by using gasoline, 70% (24/34) with diesel oil, 85% (29/34) with hexane, and 82% (28/34) with benzene. It was found that biosurfactant-producing isolates are extractable with HCl (100%), ammonium sulphate (95%), chloroform (95%), and ethanol (100%). Biosurfactants showed stability at 20°C, 37°C, 40°C, and 60°C. Biosurfactant secreted by Bacillus strains has shown an antagonistic effect in Escherichia coli, Shigella flexneri 5a M90T, and Bacillus cereus. The selected isolates could therefore be safely used for biodegradation. Substrate biodegradation patterns by individual isolates were found to significantly differ. The study shows that benzene was degraded faster, followed by hexane, gasoline, and finally diesel. The Bacillus consortium used can decrease hydrocarbon content from 195 to 112 (g/kg) in 15 days.

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Section: Discussionmentioning

confidence: 90%

“…It has been reported that Bacillus sp. are among the most predominant isolates of crude oil [2,40,41]. e phenotypic and biochemical characteristics of the 34 isolates were related and oriented towards genus Bacillus.…”

Section: Discussionmentioning

confidence: 97%

Profiling of Indigenous Biosurfactant-Producing Bacillus Isolates in the Bioremediation of Soil Contaminated by Petroleum Products and Olive Oil

Elenga-Wilson

Kayath

Mokemiabeka

et al. 2021

International Journal of Microbiology

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“…Petroleum-degrading bacteria can be used for bioremediation of soil contaminated with petroleum sludge, which is the oily sludge generated in refineries when inlet and outlet tanks are cleaned. Disposal of the sludge causes environmental issues as well as human health concerns, but bacterial remediation of contaminated soil can restore its ability to support healthy growth of crop plants (Varma et al, 2017). Successful, and rapid, degradation rates of Total Petroleum Hydrocarbon (TPH) has also been demonstrated by mixed populations of petroleum-degrading bacteria with white-rot fungi grown in solid-state fermentation.…”

Section: Fungi For Bioremediationmentioning

confidence: 99%

21st century miniguide to fungal biotechnology

et al. 2019

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Realising the biotechnological potential of fungi requires full appreciation of the molecular biology and genetics of this kingdom. We review recent advances in our understanding of fungal genetic structure as it might influence biotechnology; including introns, alternative splicing of primary transcripts, transposons (transposable elements, or TEs), heterokaryosis, ploidy and genomic variation, sequencing, annotation and comparison of fungal genomes, and gene editing. We end by indicating under-researched, but unique, aspects of fungal cell biology that offer opportunities for developing new strategies to manage the activities of fungi to our benefit. As a closing example, we discuss the potential of bioengineering fungi specifically for bioremediation of plastic wastes.

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“…在沾3油藏中还发现大量的厚壁菌门, 主要包括芽孢杆菌纲、梭菌纲以及柔膜菌纲. 目前已有大量研究发现了芽孢杆菌纲以及梭菌纲对石油烃具有较强的降解能力 [33,34] , 并能够被用于油藏开采或土壤、海洋石油污染的修复, 因为它们能够快速适应石油污染环境并利用石油进行自身生长代谢 [34] ; 其代谢产物不仅能够起到原油乳化剂的作用 [33] , 还能够为其他在油藏环境中生存但不能利用烷烃的群落提供营养 [35] . 因此, 厚壁菌门对油藏微生物群落的构建及稳定起着十分重要的作用.…”

Section: 微生物组α多样性unclassified