Degree of crosslinking of collagen at interfaces: Adhesion and shear rheological indicators

Fathima, Nishter Nishad; Dhathathreyan, Aruna; Ramasami, T.; Krägel, J.; Miller, R.

doi:10.1016/j.ijbiomac.2010.09.017

Cited by 32 publications

(16 citation statements)

References 22 publications

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“…This acid soluble rat tail tendon collagen type I was isolated according to the method described elsewhere (27). Acetic acid extraction and salting out with NaCl is included in the procedure.…”

Section: Isolation Of Collagen Type Imentioning

confidence: 99%

Collagen-nanoparticle Interactions: Type I Collagen Stabilization Using Functionalized Nanoparticles

et al. 2015

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Collagen can absorb and associate with nanoparticles, which can have significant impact on the biological behavior of collagen and nanoparticles in a biological fluid. Iron oxide nanoparticles encapsulated with polystyrene-block-poly(acrylic acid) has been synthesized and characterized. Viscosity, circular dichroism, and Fourier transform infrared spectroscopy techniques have been used to study the Fe 2 O 3 -PS-b-PAA-collagen interaction. Encapsulated nanoparticles do not alter the collagen conformational stability and changes in functional groups are due to the aggregation of protein onto the surface of nanoparticle. The ability of PS-b-PAA encapsulated nanoparticles to maintain the collagen properties will have impact in the biomedical applications of the same.

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Section: Isolation Of Collagen Type Imentioning

confidence: 99%

Collagen-nanoparticle Interactions: Type I Collagen Stabilization Using Functionalized Nanoparticles

et al. 2015

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“…Alterations in mechanical properties of natural and synthetic biomaterials can be introduced by physical, enzymatic, or chemical crosslinking [25–27]. Genipin (GP) is a non-toxic, natural crosslinker that is derived from the fruit of gardenia extracts.…”

Section: Introductionmentioning

confidence: 99%

Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering

Frohbergh¹,

Katsman²,

Botta³

et al. 2012

Biomaterials

373

201

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Reconstruction of large bone defects remains problematic in orthopedic and craniofacial clinical practice. Autografts are limited in supply and are associated with donor site morbidity while other materials show poor integration with the host’s own bone. This lack of integration is often due to the absence of periosteum, the outer layer of bone that contains osteoprogenitor cells and is critical for the growth and remodeling of bone tissue. In this study we developed a one-step platform to electrospin nanofibrous scaffolds from chitosan, which also contain hydroxyapatite nanoparticles and are crosslinked with genipin. We hypothesized that the resulting composite scaffolds represent a microenvironment that emulates the physical, mineralized structure and mechanical properties of non-weight bearing bone extracellular matrix while promoting osteoblast differentiation and maturation similar to the periosteum. The ultrastructure and physicochemical properties of the scaffolds were studied using scanning electron microscopy and spectroscopic techniques. The average fiber diameters of the electrospun scaffolds were 227±154 nm as spun, and increased to 335±119 nm after crosslinking with genipin. Analysis by X-ray diffraction, Fourier transformed infrared spectroscopy and energy dispersive spectroscopy confirmed the presence of characteristic features of hydroxyapatite in the composite chitosan fibers. The Young’s modulus of the composite fibrous scaffolds was 142±13 MPa, which is similar to that of the natural periosteum. Both pure chitosan scaffolds and composite hydroxyapatite-containing chitosan scaffolds supported adhesion, proliferation and osteogenic differentiation of mouse 7F2 osteoblast-like cells. Expression and enzymatic activity of alkaline phosphatase, an early osteogenic marker, were higher in cells cultured on the composite scaffolds as compared to pure chitosan scaffolds, reaching a significant, 2.4 fold, difference by day 14 (p<0.05). Similarly, cells cultured on hydroxyapatite-containing scaffolds had the highest rate of osteonectin mRNA expression over 2 weeks, indicating enhanced osteoinductivity of the composite scaffolds. Our results suggest that crosslinking electrospun hydroxyapatite-containing chitosan with genipin yields bio-composite scaffolds, which combine non-weight-bearing bone mechanical properties with a periosteum-like environment and facilitate the proliferation, differentiation and maturation of osteoblast-like cells. We propose that these scaffolds might be useful for the repair and regeneration of maxillofacial defects and injuries.

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“…[134,135] Tannic acid was used for many decades in the treatment of burns. [136][137][138][139] In biomaterial science, it was studied as a collagen [140][141][142][143][144][145][146][147][148][149][150][151][152] and chitosan, [153][154][155][156][157][158][159][160] as well as their mixture [161] cross-linker. Tannic acid has antioxidant and antimicrobial properties.…”

Section: Tannic Acidmentioning

confidence: 99%

Chitosan/collagen blends with inorganic and organic additive—A review

Kaczmarek

Sionkowska

2017

Adv Polym Technol

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This review provides a report on recent studies of composite materials based on a chitosan and collagen mixture due to their biocompatibility and bioactivity. Those natural polymers are most widely used to obtain biomaterials. However, the materials based on chitosan/collagen complexes present poor mechanical parameters, as well as high swelling behavior and enzymatic degradation rate. Such properties are considered problematic for the material clinical application. An increased interest in composite materials has been observed due to their enhanced physicochemical properties. A composite material preparation involves different compounds addition to natural polymers. Herein, the use of organic and inorganic additives has been reported. Moreover, the potential application of composite materials based on chitosan/ collagen complexes in the tissue engineering science has been discussed. K E Y W O R D Schitosan, collagen, composite materials, tissue engineering How to cite this article: Kaczmarek B, Sionkowska A. Chitosan/collagen blends with inorganic and organic additive-A review. Adv Polym Technol.

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Degree of crosslinking of collagen at interfaces: Adhesion and shear rheological indicators

Cited by 32 publications

References 22 publications

Collagen-nanoparticle Interactions: Type I Collagen Stabilization Using Functionalized Nanoparticles

Collagen-nanoparticle Interactions: Type I Collagen Stabilization Using Functionalized Nanoparticles

Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering

Chitosan/collagen blends with inorganic and organic additive—A review

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