Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of Barley (Hordeum vulgare L.)

Tommasini, Livia; Svensson, Jan T.; Rodriguez, Edmundo; Wahid, Abdul; Malatrasi, Marina; Kato, Kenji; Wanamaker, Steve; Resnik, Josh; Close, Timothy J.

doi:10.1007/s10142-008-0081-z

Cited by 146 publications

(109 citation statements)

References 65 publications

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“…Drought stress (DS) and low temperature stress (LTS) are among the important environmental factors that limit plant growth and productivity (Tommasini et al, 2008). Seedling establishment is a critical process to plant growth, especially under adverse environmental conditions (Bohnert et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Effects of low temperature and drought on the physiological and growth changes in oil palm seedlings

Cao¹,

Sun²,

Shao³

et al. 2011

Afr. J. Biotechnol.

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Water deficiency and low temperature are two important ecological factors which affect the distribution and cultivation of oil palm. To find out how oil palm adapts to the environmental conditions, the dynamics of a series of important physiological components derived from the leaves of potted oil palm seedlings under drought stress (DS) (water with holding) and low temperature stress (LTS) (10°C) were studied. The results showed that low temperature and water stress inhibited the growth of oil palm seedlings. The relative conductivity, injury index, malondialdehyde (MDA) and proline content in the leaves increased to different degrees with the extension of low temperature and drought stress. Superoxide dismutase (SOD) and peroxidase (POD) activities increased and then decreased gradually with the duration of treatment time. The variations of the earlier mentioned parameters except proline content under low temperature stress were greater than that under drought stress. Thus, oil palm possibly showed different response mechanisms under low temperature and drought stress by mediations of these substances, in order to increase plant defense capability. These data provided the information that was utilized to initiate the breeding programme used to improve drought and cold tolerance in oil palm.

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Section: Introductionmentioning

confidence: 99%

Effects of low temperature and drought on the physiological and growth changes in oil palm seedlings

Cao¹,

Sun²,

Shao³

et al. 2011

Afr. J. Biotechnol.

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“…DHNs are encoded by a dispersed multigene family and are differentially regulated, at least in higher plants. For example, 13 Dhn genes have been identified in barley (Hordeum vulgare), dispersed over seven genetic map locations (Choi et al, 1999;Svensson et al, 2002) and regulated variably by drought, low temperature, and embryo development (Tommasini et al, 2008). DHNs are localized in various subcellular compartments, including cytosol (Roberts et al, 1993), nucleus (Houde et al, 1995), chloroplast (Artus et al, 1996), vacuole (Heyen et al, 2002), and proximal to the plasma membrane and protein bodies (Asghar et al, 1994;Egerton-Warburton et al, 1997;Puhakainen et al, 2004).…”

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confidence: 99%

The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

et al. 2009

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Dehydrins (DHNs; late embryogenesis abundant D11 family) are a family of intrinsically unstructured plant proteins that accumulate in the late stages of seed development and in vegetative tissues subjected to water deficit, salinity, low temperature, or abscisic acid treatment. We demonstrated previously that maize (Zea mays) DHNs bind preferentially to anionic phospholipid vesicles; this binding is accompanied by an increase in α-helicity of the protein, and adoption of α-helicity can be induced by sodium dodecyl sulfate. All DHNs contain at least one “K-segment,” a lysine-rich 15-amino acid consensus sequence. The K-segment is predicted to form a class A2 amphipathic α-helix, a structural element known to interact with membranes and proteins. Here, three K-segment deletion proteins of maize DHN1 were produced. Lipid vesicle-binding assays revealed that the K-segment is required for binding to anionic phospholipid vesicles, and adoption of α-helicity of the K-segment accounts for most of the conformational change of DHNs upon binding to anionic phospholipid vesicles or sodium dodecyl sulfate. The adoption of structure may help stabilize cellular components, including membranes, under stress conditions.

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“…It seems that accumulation of dehydrins is a common feature of cold acclimation and onset of dormancy, and levels of dehydrin transcripts stay very high during the winter (Wisniewski et al 1996;Yakovlev et al 2008). Many studies report a positive correlation between the accumulation of dehydrin transcripts or proteins and tolerance to freezing, drought, or salinity (Karlson et al 2003;Kosová et al 2007;Mehta et al 2009;Rampino et al 2006;Rorat et al 2006;Tommasini et al 2008;Wisniewski et al 2008;Hara 2010). Joosen et al (2006) have demonstrated a positive correlation between cold acclimation and upregulation of K 2 type dehydrins.…”

Section: Discussionmentioning

confidence: 99%

Dehydrins in maritime pine (Pinus pinaster) and their expression related to drought stress response

Velasco-Conde

Yakovlev²,

Majada

et al. 2012

Tree Genetics & Genomes

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Maritime pine (Pinus pinaster) is an important commercial species throughout its Atlantic distribution. With the anticipated increase in desiccation of its habitat as a result of climate change, the selection of genotypes with increased survival and growth capability under these conditions for breeding programs is of great interest for this species. We aimed to study the response to a realistic drought stress under controlled conditions, looked for a method to measure dehydration resistance, and analyzed dehydrin expression in drought-resistant and drought-sensitive clones from different ecotypes. We report here the sequence characteristics and the expression patterns of five dehydrins from P. pinaster, along with the physiological characterization of drought stress responses in different genotypes (clonally replicated plants), originating from a broad geographical distribution across France and Spain (provenances). In total, we distinguished five different dehydrin genes in silico, grouped into two types-K 2 and SK n . Three of the dehydrin genes had several sequence variants, differing by multiple or single amino acid substitutions. Only two of the dehydrins (PpinDhn3 and PpinDhn4) showed an increase in transcription with increased drought stress which was dependent on provenance and genotype, suggesting their involvement in drought resistance. The other dehydrins showed decreased expression trends with increased severity of the drought stress. The lack of close association between the drought stress and expression patterns of these dehydrin genes suggest that they could have other functions and not be involved in drought resistance. Our results suggest large differences in function between different dehydrin genes.

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Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of Barley (Hordeum vulgare L.)

Cited by 146 publications

References 65 publications

Effects of low temperature and drought on the physiological and growth changes in oil palm seedlings

Effects of low temperature and drought on the physiological and growth changes in oil palm seedlings

The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

Dehydrins in maritime pine (Pinus pinaster) and their expression related to drought stress response

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