Deletion of the Infectious spleen and kidney necrosis virus ORF069L reduces virulence to mandarin fish Siniperca chuatsi

Lin, Yifan; Zeng, Ruoyun; Li, Zhimin; Luo, Zhiyong; Pan, Weiqiang; Weng, Shaoping; Guo, Chang-Jun; He, Jianguo

doi:10.1016/j.fsi.2019.10.039

Cited by 14 publications

(7 citation statements)

References 22 publications

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“…The ISKNV(NC_003494.1) was originally isolated from disease-infected mandarin fish [ 13 ]. The ISKNV strain used in this study was separated and obtained from the Nanhai mandarin fish farm in 2017 and stored in our laboratory [ 29 ]. The whole genome of this ISNKV strain has no significant difference to ISKNV(NC_003494.1).…”

Section: Methodsmentioning

confidence: 99%

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Chen

Wang

et al. 2021

IJMS

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Exosomes are associated with cancer progression, pregnancy, cardiovascular diseases, central nervous system-related diseases, immune responses and viral pathogenicity. However, study on the role of exosomes in the immune response of teleost fish, especially antiviral immunity, is limited. Herein, serum-derived exosomes from mandarin fish were used to investigate the antiviral effect on the exosomes of teleost fish. Exosomes isolated from mandarin fish serum by ultra-centrifugation were internalized by mandarin fish fry cells and were able to inhibit Infectious spleen and kidney necrosis virus (ISKNV) infection. To further investigate the underlying mechanisms of exosomes in inhibiting ISKNV infection, the protein composition of serum-derived exosomes was analyzed by mass spectrometry. It was found that myxovirus resistance 1 (Mx1) was incorporated by exosomes. Furthermore, the mandarin fish Mx1 protein was proven to be transferred into the recipient cells though exosomes. Our results showed that the serum-derived exosomes from mandarin fish could inhibit ISKNV replication, which suggested an underlying mechanism of the exosome antivirus in that it incorporates Mx1 protein and delivery into recipient cells. This study provided evidence for the important antiviral role of exosomes in the immune system of teleost fish.

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Section: Methodsmentioning

confidence: 99%

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Chen

Wang

et al. 2021

IJMS

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“…The ISKNV strain (NH-2017) was isolated from the spleen of the moribund mandarin fish with symptoms of ISKNV infection in Nanhai fish farm, which was provided by associate Prof. Shao-Ping Weng in 2017 [ 24 ]. MFF-1 cells were seeded in plates for 24 h, and then exposed to ISKNV at 1 µl 1.196 × 10 9 TCID 50 /mL dilution in 1 mL of culture medium.…”

Section: Methodsmentioning

confidence: 99%

Hypoxia triggers the outbreak of infectious spleen and kidney necrosis virus disease through viral hypoxia response elements

Liu

et al. 2022

Virulence

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Hypoxia frequently occurs in aquatic environments, especially in aquaculture areas. However, research on the relationship between hypoxic aquatic environments with viral diseases outbreak is limited, and its underlying mechanisms remain elusive. Herein, we demonstrated that hypoxia directly triggers the outbreak of infectious spleen and kidney necrosis virus (ISKNV) disease. Hypoxia or activated hypoxia-inducible factor (HIF) pathway could remarkably increase the levels of viral genomic DNA, titers, and gene expression, indicating that ISKNV can response to hypoxia and HIF pathway. To reveal the mechanism of ISKNV respond to HIF pathway, we identified the viral hypoxia response elements (HREs) in ISKNV genome. Fifteen viral HREs were identified, and four related viral genes responded to the HIF pathway, in which the hre-orf077r promoter remarkably responded to the HIF pathway. The level of orf077r mRNA dramatically increased after the infected cells were treated with dimethyloxalylglycine (DMOG) or the infected cells/fish subjected to hypoxic conditions, and overexpressed orf077r could remarkably increase the ISKNV replication. These finding shows that hypoxic aquatic environments induce the expression of viral genes through the viral HREs to promote ISKNV replication, indicating that viral HREs might be important biomarkers for the evaluation of the sensitivity of aquatic animal viral response to hypoxia stress. Furthermore, the frequencies of viral HREs in 43 species aquatic viral genomes from 16 families were predicted and the results indicate that some aquatic animal viruses, such as Picornavirdea, Dicistronviridae , and Herpesviridae , may have a high risk to outbreak when the aquatic environment encounters hypoxic stress.

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“…The WT ISKNV strain used for the construction of DORF022L was separated from mandarin fish in 2006 (Guo et al, 2011) and stored in the laboratory, while that used in the challenge experiment was obtained in 2017 (Lin et al, 2019).…”

Section: Star+methodsmentioning

confidence: 99%

“…Spleen samples (n=3) were collected at 0, 3, 6, 9, 12, 15, and 18 days post-infection, with three fish samples per day from each group. The levels of the immune-related genes (IFN-c, IFN-h, IFN-d, IL-18, IgM, TNF-a, and IL-1) were quantified via qRT-PCR methods, as described previously (He et al, 2019). The primers used are listed in Table S2.…”

Section: Star+methodsmentioning

confidence: 99%

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Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection

et al. 2021

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Deletion of the Infectious spleen and kidney necrosis virus ORF069L reduces virulence to mandarin fish Siniperca chuatsi

Cited by 14 publications

References 22 publications

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Hypoxia triggers the outbreak of infectious spleen and kidney necrosis virus disease through viral hypoxia response elements

Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection

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