Deltonin, a Steroidal Saponin, Inhibits Colon Cancer Cell Growth &lt;i&gt;in Vitro&lt;/i&gt; and Tumor Growth &lt;i&gt;in Vivo&lt;/i&gt; via Induction of Apoptosis and Antiangiogenesis

Tong, Qingyi; Qing, Yong; Shu, Dan; He, Yang; Zhao, Ying; Li, Yi; Wang, Zhen Ling; Zhang, Shi Yuan; Xing, Zhi Hua; Cheng, Xu; Wei, Yu Quan; Huang, Wen; Xiao, Wei

doi:10.1159/000327949

Cited by 31 publications

(15 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, echinoside A, a saponin from the sea cucumber H. nobilis Selenka, induced apoptosis and inhibited tumor growth in a nude mouse xenograft tumor model by specifically antagonizing the binding of topoisomerase 2α to DNA, which is based on a highly enzyme-specific interaction. Similar effects were observed for deltonin, 62 alpha-tomatine, 23 and tubeimoside-1.…”

Section: Efficacy Of Saponins and Clinical Potentialsupporting

confidence: 80%

“…For instance, deltonin, a steroidal monodesmosidic saponin from Dioscorea zingiberensis Wright, hampered tumor growth in tumor-bearing BALB/c mice after oral administration. 62 Similarly, timosaponin A-III, a steroidal saponin from Anemarrhena sphodeloides Bunge, slowed tumor growth in a xenograft tumor model in BALB/c-nu mice, 63 and ginsenoside Rg3, from the American ginseng, Panax quinquefolius L, inhibited growth of human colon cancer cells in a xenograft tumor model. 23 Suppressed tumor growth was also observed for other saponins like 51,52 and several other saponins.…”

Section: Efficacy Of Saponins and Clinical Potentialmentioning

confidence: 99%

See 1 more Smart Citation

Chemistry and pharmacology of saponins: special focus on cytotoxic properties

Thakur¹,

Melzig²,

Fuchs³

et al. 2011

BTAT

View full text Add to dashboard Cite

Saponins are bioactive compounds produced mainly by plants but also by some marine organisms and insects. In the recent past, there has been unforeseen interest in the clinical utilization of saponins as chemotherapeutic agents. The research on saponins in various forms as a treatment for cancer has generated a lot of potential. The advent of nanotechnology and the cytotoxicity enhancing properties of saponins are some of the highlights of the current decade. This review gives an updated overview of the clinical potential that saponins hold as cytotoxic agents, and covers the literature for 1957-2011, with the main focus on research conducted in the last decade. It is conceivable that saponins hold a lot of therapeutic potential and could be a lead for identification of synthetic or semisynthetic molecules for the treatment of cancer via membrane-mediated or transport-mediated pathways.

show abstract

Section: Efficacy Of Saponins and Clinical Potentialsupporting

confidence: 80%

Section: Efficacy Of Saponins and Clinical Potentialmentioning

confidence: 99%

Chemistry and pharmacology of saponins: special focus on cytotoxic properties

Thakur¹,

Melzig²,

Fuchs³

et al. 2011

BTAT

View full text Add to dashboard Cite

show abstract

“…Deltonin was obtained as previously described and its purity determined by high performance liquid chromatography (>98%) (19 (12, 24, 36, 48 h). All experiments were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…Deltonin, diosge nin- (15)(16)(17)(18). We have previously demonstrated that deltonin has cytotoxic effects against colon cancer cell lines and oral administration of deltonin was found to significantly inhibit tumor growth and prolonged survival of tumor bearing mice (19,20). However, the effects of deltonin in breast cancer remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Deltonin induces apoptosis in MDA-MB-231 human breast cancer cells via reactive oxygen species-mediated mitochondrial dysfunction and ERK/AKT signaling pathways

Zhang

Tong

et al. 2013

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

Abstract. Deltonin, a steroidal saponin isolated from Dioscorea zingiberensis Wright, exhibits high cytotoxic activity in cancer cells. In the present study, the effects of deltonin on cell proliferation and apoptosis were evaluated in the MDA-MB-231 human breast carcinoma cell line. Following treatment with deltonin, the viability of MDA-MB-231 cells was analyzed using MTT assay and apoptosis, mitochondrial membrane potential (∆Ψm) alternation and intracellular reactive oxygen species (ROS) generation was determined by flow cytometry. In addition, western blot analysis was performed to examine the expression of apoptosis-associated proteins. The results demonstrated that deltonin induced apoptosis in MDA-MB-231 cells in a time-and concentration-dependent manner. Apoptosis was associated with depolarization of ∆Ψm and time-dependent ROS generation. Deltonin treatment also resulted in Bax upregulation, Bcl-2 downregulation, activation of caspase-3 and -8 and poly (ADP ribose) polymerase cleavage. Decreased levels of phosphorylated extracellular signal-regulated kinase (ERK) and phosphorylated AKT were also observed. Results indicate that the proliferation inhibitory effect of deltonin is associated with its apoptosis-inducing effect, which may correlate with ROS-mediated mitochondrial dysfunction as well as activation of the ERK/AKT signaling pathways. Therefore, deltonin may be a potential chemotherapeutic agent for the treatment of breast cancer. IntroductionBreast cancer is the most commonly diagnosed cancer and the leading cause of cancer mortality among females, accounting for 23% of total cancer cases and 14% of cancer mortalities (1). In developing countries, including China, breast cancer remains a significant public health issue due to its prevalence (2). In recent years, medical advances have increased the availablity and efficacy of breast cancer treatments, including improved surgical methods for lumpectomy and mastectomy and radiation, hormone and chemotherapies. However, the majority of drugs currently used as chemotherapeutic agents exhibit low efficacy and are associated with the development of drug resistance (3,4). Therefore, new drugs with a higher therapeutic index are urgently required to effectively treat this malignancy.Over the last two decades, an increasing number of bioactive compounds have been identified in traditional Chinese medical herbs (5). Specific compounds have been reported to kill tumor cells by generation of reactive oxygen species (ROS). ROS are known to affect mitochondrial membrane potential (∆Ψm) and trigger a series of mitochondria-associated events (6,7). The generation of ROS may contribute to mitochondrial damage, reduction of ∆Ψm, release of cytochrome c and Smac and subsequent caspase activation and apoptosis (8,9). Apoptosis is a major control mechanism of cell death when DNA damage is not repaired. Apoptosis is a gene-directed programmed cell death characterized by cell shrinkage, blebbing of the plasma membrane, chromosomal DNA fragmentation and a number of oth...

show abstract

“…The observed biological activities of T. terrestris are mainly due to its abundant steroidal saponins [10,11,12,13]. Researchers have focused on steroidal saponins as potential candidates for the development of chemotherapeutic agents due to their anticancer effects [14,15,16]. However, the potential anticancer effects of terrestrosin D (TED; fig.…”

Section: Introductionmentioning

confidence: 99%

Terrestrosin D, a Steroidal Saponin from <b><i>Tribulus terrestris</i></b> L., Inhibits Growth and Angiogenesis of Human Prostate Cancer in vitro and in vivo

Wei¹,

Fukuhara²,

Chen

et al. 2014

Pathobiology

View full text Add to dashboard Cite

Objective: The aim of this study was to investigate whether terrestrosin D (TED) inhibits the progression of castration-resistant prostate cancer and consider its mechanism. Methods: Cell cycle, mitochondrial membrane potential (ΔΨm) and apoptosis were determined by flow cytometry. Caspase-3 activity and vascular endothelial growth factor secretion were detected by a caspase-3 assay and human vascular endothelial growth factor kit, respectively. A PC-3 xenograft mouse model was used to evaluate the anticancer effect of TED in vivo. Results: In vitro, TED strongly suppressed the growth of prostate cancer cells and endothelial cells in a dose-dependent manner. TED induced cell cycle arrest and apoptosis in PC-3 cells and human umbilical vascular endothelial cells (HUVECs). TED-induced apoptosis did not involve the caspase pathway. TED also decreased ΔΨm in PC-3 cells and HUVECs. In vivo, TED significantly suppressed tumor growth in nude mice bearing PC-3 cells, without any overt toxicity. Immunohistochemical analysis showed TED induced apoptotic cell death and inhibited angiogenesis in xenograft tumor cells. Conclusion: Cell cycle arrest and induction of apoptosis in cancer cells and endothelial cells might be plausible mechanisms of actions for the observed antitumor and antiangiogenic activities of TED.

show abstract

Deltonin, a Steroidal Saponin, Inhibits Colon Cancer Cell Growth <i>in Vitro</i> and Tumor Growth <i>in Vivo</i> via Induction of Apoptosis and Antiangiogenesis

Cited by 31 publications

References 39 publications

Chemistry and pharmacology of saponins: special focus on cytotoxic properties

Chemistry and pharmacology of saponins: special focus on cytotoxic properties

Deltonin induces apoptosis in MDA-MB-231 human breast cancer cells via reactive oxygen species-mediated mitochondrial dysfunction and ERK/AKT signaling pathways

Terrestrosin D, a Steroidal Saponin from <b><i>Tribulus terrestris</i></b> L., Inhibits Growth and Angiogenesis of Human Prostate Cancer in vitro and in vivo

Contact Info

Product

Resources

About