Demethoxycurcumin mediated targeting of MnSOD leading to activation of apoptotic pathway and inhibition of Akt/NF-κB survival signalling in human glioma U87 MG cells

Kumar, Rakesh; Lal, Neetika; Nemaysh, Vishal; Luthra, Pratibha Mehta

doi:10.1016/j.taap.2018.02.020

Cited by 28 publications

(17 citation statements)

References 51 publications

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“…In vitro studies, using both human (T98G, U87MG, T67, U373) and rat (C6) GBM cell lines, demonstrated that curcumin and curcuminoids (i.e., demethoxycurcumin (DMC)) reduce cell survival through the suppression of both NF-κB and AP-1 signaling activation. This prevents the constitutive activation of Akt and JNK [ 45 , 100 , 101 , 123 , 124 ]. At the same time, the curcumin-dependent inhibition of AP-1 signaling suppresses MMPs transcription, thus markedly repressing GBM cells invasive potential [ 122 ].…”

Section: Effects Of Curcumin On Gbm Beyond Atg Inductionmentioning

confidence: 99%

“…Among the pleiotropic activities exerted by curcumin, the anti-proliferative effects of this natural polyphenol rely, at least in part, on the activation of pro-apoptotic pathways. In fact, several studies demonstrate that curcumin-induced G2/M cell cycle arrest frequently occurs along with an induction of caspase-mediated cell death [ 46 , 123 , 127 , 129 ]. Remarkably, curcumin induces apoptosis via a caspase-dependent pathway in human GBM cells [ 46 , 130 ].…”

Section: Effects Of Curcumin On Gbm Beyond Atg Inductionmentioning

confidence: 99%

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The Multi-Faceted Effect of Curcumin in Glioblastoma from Rescuing Cell Clearance to Autophagy-Independent Effects

et al. 2020

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The present review focuses on the multi-faceted effects of curcumin on the neurobiology glioblastoma multiforme (GBM), with a special emphasis on autophagy (ATG)-dependent molecular pathways activated by such a natural polyphenol. This is consistent with the effects of curcumin in a variety of experimental models of neurodegeneration, where the molecular events partially overlap with GBM. In fact, curcumin broadly affects various signaling pathways, which are similarly affected in cell degeneration and cell differentiation. The antitumoral effects of curcumin include growth inhibition, cell cycle arrest, anti-migration and anti-invasion, as well as chemo- and radio-sensitizing activity. Remarkably, most of these effects rely on mammalian target of rapamycin (mTOR)-dependent ATG induction. In addition, curcumin targets undifferentiated and highly tumorigenic GBM cancer stem cells (GSCs). When rescuing ATG with curcumin, the tumorigenic feature of GSCs is suppressed, thus counteracting GBM establishment and growth. It is noteworthy that targeting GSCs may also help overcome therapeutic resistance and reduce tumor relapse, which may lead to a significant improvement of GBM prognosis. The present review focuses on the multi-faceted effects of curcumin on GBM neurobiology, which represents an extension to its neuroprotective efficacy.

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Section: Effects Of Curcumin On Gbm Beyond Atg Inductionmentioning

confidence: 99%

Section: Effects Of Curcumin On Gbm Beyond Atg Inductionmentioning

confidence: 99%

The Multi-Faceted Effect of Curcumin in Glioblastoma from Rescuing Cell Clearance to Autophagy-Independent Effects

et al. 2020

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“…Several studies showed that MMP-9 was detected in 50 % of GBM patients. Also, increased MMP-2 was found in 52 % of GBM patients [39]. Xue et al in their study evaluated the high expression of MMP-9 in glioma, and it is associated with patient survival rates [40].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Effects of Ferula Latisecta on Human Glioma U87 Cells

et al. 2019

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Glioblastoma multiforme (GBM) is the fatal type of astrocytic tumors with a survival rate of 12 months. The present study, for the first time, evaluated the cytotoxic impacts of Ferula latisecta (F. latisecta) hydroalcoholic extract on U87 GBM cell line. The MTT assay measured the cellular toxicity following 24- and 48 h treatment with various doses of F. latisecta (0–800 μg/mL). Apoptosis was evaluated by an Annexin V/propidium iodide (PI) staining 24 h after treatment by F. latisecta. Moreover, to determine the cellular metastasis of U87 cells, we used a gelatin zymography assay (matrix metalloproteinase [MMP]-2/-9 enzymatic activity). The outcomes showed that F. latisecta mitigated the viability of U87 cells in a concentration- and time-dependent manner with IC50 values of 145.3 and 192.3 μg/mL obtained for 24- and 48 h treatments, respectively. F. latisecta induced apoptosis in a concentration-dependent manner after 24 h. Also, MMP-9 activity was significantly decreased following 24 h after treatment concentration-dependently with no change in MMP-2 enzymatic activity. This study showed that F. latisecta induced cytotoxicity and apoptosis, and mitigated metastasis of U87 GBM cells. Hence, F. latisecta could be beneficial as a promising natural herb against GBM after further studies.

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“…Bisdemethoxycurcumin, acting like curcumin, could directly neutralize free radicals and interrupt the chain reaction [7]. On the other hand, the induction of curcuminoids on the antioxidant enzyme activities, like SOD, has been widely reported [24,25,26]. The increased SOD in AAPH-treated erythrocytes might result from their indirect free radical scavenging property.…”

Section: Discussionmentioning

confidence: 99%

Comparative Studies on the Antioxidant Profiles of Curcumin and Bisdemethoxycurcumin in Erythrocytes and Broiler Chickens

Zhang

Han

Shen

et al. 2019

Animals

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Simple SummaryTurmeric, which is the rhizome of Curcuma longa, has a long history for spice and medicine in China, India, and other tropical countries. Curcuminoids, as the principle active compounds of turmeric, consist of curcumin (about 77%), demethoxycurcumin (about 17%), and bisdemethoxycurcumin (about 3%). Studies showed that curcuminoids, especially curcumin, possesses antioxidant, free radical scavenging activities, and thus have a health-promoting effect in human and animals. Of the three curcuminoids, extensive research on the biological activity of curcumin was carried out for decades. However, its natural analogues bisdemethoxycurcumin was relatively less investigated. Based the records, there was still controversy regarding the relative potency of antioxidant activity of curcuminoid that is dependent on different cell types and animal models, which ultimately affected their beneficial effects on the intestinal health and animal production as well. Thus, whether curcumin and bisdemethoxycurcumin shared the same efficiency of antioxidant activity in chicken erythrocytes and broiler chickens remains unknown. Our results demonstrated, for the first time, that the bisdemethoxycurcumin, acting like curcumin, exerted good free radical scavenging activity in erythrocytes and improved the redox status in broilers, although there were some slight differences in their efficiency of antioxidant activities in broiler chickens.AbstractThe aim of this study was to investigate the antioxidant effects of curcumin and bisdemethoxycurcumin in both 2,20-azobis(2-amidinopropane) dihydrochloride (AAPH)-treated erythrocytes and broiler chickens. In experiment 1, chicken erythrocytes were employed to determine the antioxidant protection against AAPH treatment. Significant differences in hemolysis, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were observed between the control and curcuminoids-treated groups. In experiment 2, a total of 480 Arbor Acres broilers with the similar body weights were used. All of the birds were fed basal diet and basal diet with 150 mg/kg curcumin or bisdemethoxycurcumin, respectively. The results showed that curcuminoids significantly increased ADG, serum antioxidant capacity, the glutathione redox potential of small intestine, the gene expression of Nrf2, and its related antioxidant enzymes. Besides, curcumin and bisdemethoxycurcumin increased the antioxidant activities of serum, diet, and excreta while using the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and ferric-reducing antioxidant power methods. It was concluded that bisdemethoxycurcumin, acting like curcumin, exerted good free radical scavenging activity in erythrocytes and improved the redox status in broilers, although there were some slight differences in their efficiency of antioxidant activities.

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Demethoxycurcumin mediated targeting of MnSOD leading to activation of apoptotic pathway and inhibition of Akt/NF-κB survival signalling in human glioma U87 MG cells

Cited by 28 publications

References 51 publications

The Multi-Faceted Effect of Curcumin in Glioblastoma from Rescuing Cell Clearance to Autophagy-Independent Effects

The Multi-Faceted Effect of Curcumin in Glioblastoma from Rescuing Cell Clearance to Autophagy-Independent Effects

Cytotoxic Effects of Ferula Latisecta on Human Glioma U87 Cells

Comparative Studies on the Antioxidant Profiles of Curcumin and Bisdemethoxycurcumin in Erythrocytes and Broiler Chickens

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