Comparative Studies on the Antioxidant Profiles of Curcumin and Bisdemethoxycurcumin in Erythrocytes and Broiler Chickens

Abstract

Simple SummaryTurmeric, which is the rhizome of Curcuma longa, has a long history for spice and medicine in China, India, and other tropical countries. Curcuminoids, as the principle active compounds of turmeric, consist of curcumin (about 77%), demethoxycurcumin (about 17%), and bisdemethoxycurcumin (about 3%). Studies showed that curcuminoids, especially curcumin, possesses antioxidant, free radical scavenging activities, and thus have a health-promoting effect in human and animals. Of the three curcuminoids, extensive research on the biological activity of curcumin was carried out for decades. However, its natural analogues bisdemethoxycurcumin was relatively less investigated. Based the records, there was still controversy regarding the relative potency of antioxidant activity of curcuminoid that is dependent on different cell types and animal models, which ultimately affected their beneficial effects on the intestinal health and animal production as well. Thus, whether curcumin and bisdemethoxycurcumin shared the same efficiency of antioxidant activity in chicken erythrocytes and broiler chickens remains unknown. Our results demonstrated, for the first time, that the bisdemethoxycurcumin, acting like curcumin, exerted good free radical scavenging activity in erythrocytes and improved the redox status in broilers, although there were some slight differences in their efficiency of antioxidant activities in broiler chickens.AbstractThe aim of this study was to investigate the antioxidant effects of curcumin and bisdemethoxycurcumin in both 2,20-azobis(2-amidinopropane) dihydrochloride (AAPH)-treated erythrocytes and broiler chickens. In experiment 1, chicken erythrocytes were employed to determine the antioxidant protection against AAPH treatment. Significant differences in hemolysis, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were observed between the control and curcuminoids-treated groups. In experiment 2, a total of 480 Arbor Acres broilers with the similar body weights were used. All of the birds were fed basal diet and basal diet with 150 mg/kg curcumin or bisdemethoxycurcumin, respectively. The results showed that curcuminoids significantly increased ADG, serum antioxidant capacity, the glutathione redox potential of small intestine, the gene expression of Nrf2, and its related antioxidant enzymes. Besides, curcumin and bisdemethoxycurcumin increased the antioxidant activities of serum, diet, and excreta while using the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and ferric-reducing antioxidant power methods. It was concluded that bisdemethoxycurcumin, acting like curcumin, exerted good free radical scavenging activity in erythrocytes and improved the redox status in broilers, although there were some slight differences in their efficiency of antioxidant activities.

Method to Improve Azo-Compound (AAPH)-Induced Hemolysis of Erythrocytes for Assessing Antioxidant Activity of Lipophilic Compounds

“…13) For AAPH-induced oxidative hemolysis experiments, most of the published studies to date also used absorbance at about 540 nm to analyze the extent of hemolysis. 8,10,11,[14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] The absorption spectrum of erythrocytes that we observed after water-induced hemolysis had peaks at 541 and 576 nm (Fig. 1A).…”

“…Many studies have used AAPH-induced oxidative hemolysis to measure antioxidant activity of various compounds, especially complex mixture compounds. [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] In the present study, we re-examined the methodology of AAPH-induced oxidative hemolysis and propose an improved method for evaluating antioxidant activity of various compounds, especially lipophilic compounds, using AAPH-induced hemolysis of erythrocytes.…”

Effect of Dietary Curcumin on the Growth Performance, Serum Antioxidation and Meat Quality of Ducks (Anas Platyrhynchos)

“…Zhang et al [29] found that the addition of curcumin (150 mg/kg) in the diet increased the activities Zhang et al [30] found that the addition of curcumin in the diet given to broilers increased linearly and quadratically (P < 0.05) the redness values, decreased quadratically (P < 0.05) the drip loss at 24 h in broiler breast muscle, and decreased the cooking loss with no signi cant effects. Another study of Zhang et al [31] displayed that adding curcumin in the diet given to pigs signi cantly decreased the drip loss at 24 h and 48 h and increased the redness values in leg meat of growing pigs.…”

Bisdemethoxycurcumin inhibits oxidative stress and antagonizes Alzheimer's disease by up‐regulating SIRT1

“…BDMC (bisdemethoxycurcumin) is a derivative of curcumin. On the basis of curcumin matrix, BDMC removes the 3‐position methoxy group on the bilateral benzene ring and retains the 4‐position hydroxyl group (Zhang, Han, Shen, Zhang, & Wang, 2019; Figure 1). It has more efficient pharmacological properties than curcumin.…”