1975
DOI: 10.1677/joe.0.0640539
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DEMONSTRATION OF a SPECIFIC CYTOSOL RECEPTOR IN THE NORMAL AND HYPERPLASTIC CANINE PROSTATE FOR 5α-Androstane-3α, 17α-Diol

Abstract: A specific receptor protein has been demonstrated for 5alpha-androstane-3alpha, 17alpha-diol in cytoplasmic extracts of normal and hyperplastic canine prostates. The receptor molecule, with a sedimentation coefficient of 4-5S, has been identified by the use of sucrose gradient centrifugation of tissue fractions which had been labelled in vitro with tritiated 5alpha-androstane-3alpha, 17alpha-diol. The receptor showed a relatively high affinity for this compound whereas binding could not be demonstrated with ot… Show more

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Cited by 38 publications
(5 citation statements)
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“…Dithiothreitol Preparation and labelling of cytosol Overlying fat, connective tissue and the urethra were removed from the prostate glands and the glands were chopped finely. The tissue was then washed in medium A (10 mM-Tris-HCl, 0-1 mM-EDTA, 0-25 mM-dithiothreitol, pH 7-4) and homogenized as described by Evans & Pierrepoint (1975) at a tissue : medium ratio of 1 : 10 (w/v). Assessment of binding characteristics Sucrose density-gradient ultracentrifugation Cytosol (1 ml) was incubated with tritiated oestradiol-17ß, oestradiol-17 , oestrone or oestriol (0-74 nmol/1), in the presence or absence of a 100-fold excess of unlabelled steroid, at 0°C for 4-6 h. The labelled cytosol (400 µ ) was layered on to 5-20% sucrose density gradients, prepared in medium A and centrifuged at 114 000 # for 18 h at 3°C using a Beckmann SW50.1 swinging-bucket rotor in an L265B ultracentrifuge.…”
Section: Methodsmentioning
confidence: 99%
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“…Dithiothreitol Preparation and labelling of cytosol Overlying fat, connective tissue and the urethra were removed from the prostate glands and the glands were chopped finely. The tissue was then washed in medium A (10 mM-Tris-HCl, 0-1 mM-EDTA, 0-25 mM-dithiothreitol, pH 7-4) and homogenized as described by Evans & Pierrepoint (1975) at a tissue : medium ratio of 1 : 10 (w/v). Assessment of binding characteristics Sucrose density-gradient ultracentrifugation Cytosol (1 ml) was incubated with tritiated oestradiol-17ß, oestradiol-17 , oestrone or oestriol (0-74 nmol/1), in the presence or absence of a 100-fold excess of unlabelled steroid, at 0°C for 4-6 h. The labelled cytosol (400 µ ) was layered on to 5-20% sucrose density gradients, prepared in medium A and centrifuged at 114 000 # for 18 h at 3°C using a Beckmann SW50.1 swinging-bucket rotor in an L265B ultracentrifuge.…”
Section: Methodsmentioning
confidence: 99%
“…achieved by the use of dihydrodibutylstilboestrol (Pierrepoint, 1975) may have been due to its ability to compete with oestrogens for their receptor rather than for the receptor for 5a-androstane-3a,17a-diol (Evans & Pierrepoint, 1975).…”
mentioning
confidence: 99%
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“…As the deferential vein carries a higher proportion of 5oc-reduced compounds than peripheral blood ) the activity of the 5a-reductase in the prostate and any changes that take place with age may only be as important as similar levels and changes in the 3a-and/or 17a-hydroxysteroid dehydrogenases. These would promote the formation of what appears to be the specific androgen for the dog prostate, 5a-androstane-3a, 17a-diol (Evans & Pierrepoint, 1975). Increased levels of 5a-reduced steroids from the epididymis or enhanced steroid dehydrogenases in the prostate would result in the accumulation of this active androgen in the prostate gland.…”
Section: Testosterone Uptake By the Dog Prostatementioning
confidence: 99%
“…The efficacy of this procedure was assessed by electron microscopy and shown to be valid. A cytosol preparation was achieved by the method of Evans & Pierrepoint (1975) at a tissue to medium ratio of 1:3.…”
Section: Introductionmentioning
confidence: 99%