Demonstration of IgA Polioantibody in Saliva, Duodenal Fluid and Urine

Berger, Ruth; Ainbender, Eugene; Hodes, Horace L.; Zepp, Helen D.; Hevizy, Maria M.

doi:10.1038/214420a0

Cited by 61 publications

(21 citation statements)

References 5 publications

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“…In addition, no low molecular weight -yA-reactive molecules have been de- urine against E. coli. In immunized individuals yA-urinary antibodies have been demonstrated against S. typhi (28), polio virus (29), and tetanus toxoid (8). In addition, a yA-rheumatoid factor has been isolated from the urines of patients with high serum titers of rheumatoid factor and a total 24 hr urinary protein excretion of less than 80 mg (30).…”

Section: Resultsmentioning

confidence: 99%

Secretory γA in normal urine

Bienenstock¹,

Tomasi²

1968

J. Clin. Invest.

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A B S T R A C T The physicochemical nature of yA was investigated in normal male and female urine concentrated approximately 1000 times. Sucrose density gradient ultracentrifugation and Sephadex G-200 chromatography revealed that urinary yA has sedimentation properties intermediate between 19S and 7S molecules. Isolation of urinary yA by DE 52 chromatography free of other immunoglobulins with subsequent antigenic analysis showed that the urinary yA-molecule is antigenically indistinguishable from the yA-molecules found in other external secretions and has a corrected sedimentation coefficient of 11.8S. In addition, like other secretory yA-molecules and unlike serum polymeric yA, urinary yA resisted mild reductive measures with 0.1M f8-mercaptoethanol. Free or unattached secretory "piece" was found in all normal urines tested and in agammaglobulinemic urine. Secretory "piece" antigenic determinants were also found in ureteric urine. The average daily excretion of urinary yA was 1.1 mg. The maximum excretion of urinary 7S

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Section: Resultsmentioning

confidence: 99%

Secretory γA in normal urine

Bienenstock¹,

Tomasi²

1968

J. Clin. Invest.

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“…Although direct evidence for a local origin of intestinal secretory IgA is lacking, there are several indirect arguments in favor of such a view (27,31). "Coproantibodies," i.e., antibodies found in intestinal secretions and directed against antigenic material from the gut, have repeatedly been demonstrated to be of the IgA class (5)(6)(7)32).…”

Section: Role Of the Intestinal Mucosa In The Antibodymentioning

confidence: 99%

ANTIBODIES OF THE IgA TYPE IN INTESTINAL PLASMA CELLS OF GERMFREE MICE AFTER ORAL OR PARENTERAL IMMUNIZATION WITH FERRITIN

Crabbé

Nash

Bazin

et al. 1969

The Journal of Experimental Medicine

190

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In adult germfree C3H mice immunized with horse spleen ferritin, either subcutaneously or intraperitoneally, plasma cells containing specific antibodies were found in lymph nodes and spleen and, in smaller numbers, also in the lamina propria of the intestine. In extraintestinal sites, these antiferritin-containing plasma cells were mainly of the IgM class after a single stimulation, and of the IgG1 class after repeated stimulation. In the intestine, all the anti-ferritin-containing cells appeared to be of the IgA class. Circulating antibodies, after repeated stimulation, were for the major part IgG1 and IgG2. In germfree mice given ferritin in their drinking water, antiferritin-containing cells were abundant in the intestinal mucosa, much less numerous in the mesenteric lymph nodes, and extremely scarce in other lymphoid tissues. All these cells, whatever their location, appeared to belong exclusively to the IgA class. Similarly, all the circulating antibody in these animals was found to be IgA. These findings illustrate the role of the gut as a site of antibody synthesis, as well as its selective commitment to the production of antibodies of the IgA class.

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“…An tibody produced in the intestinal tract of the IgA class has been shown to provide protec tion against certain bacterial and viral agents [2,[8][9][10]15]; however, a protective role for exocrine antibodies (i.e. IgA) in gastrointestinal helminthiasis has not been elucidated [5],…”

Section: Introductionmentioning

confidence: 99%

Specific Antibody Levels in the Intestinal Perfusates of <i>Heligosomoides polygyrus</i>-lnfected Mice

Cypess¹,

Ebersole²,

Molinari³

1977

Int Arch Allergy Immunol

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Radial immunodiffusion analysis was performed on intestinal perfusates from ICR/CD₁ mice following oral infection and challenge with viable Heligosomoides polygyrus larvae. Infection of these mice resulted in an alteration in the amount and class of immunoglobulins, as well as anti-parasitic antibody. Specific IgA peaked at 3 days post-infection and then showed a gradual decline until day 21. This rapid elevation in IgA levels was temporally associated with early events in the parasite life cycle, and the increase in IgA was significantly higher in mice infected twice with H. polygyrus prior to challenge. Differences in both the concentrations and kinetics of specific IgA and IgG₁ were also observed in the perfusates from these mice when compared to those noted for animals infected only once.

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Demonstration of IgA Polioantibody in Saliva, Duodenal Fluid and Urine

Cited by 61 publications

References 5 publications

Secretory γA in normal urine

Secretory γA in normal urine

ANTIBODIES OF THE IgA TYPE IN INTESTINAL PLASMA CELLS OF GERMFREE MICE AFTER ORAL OR PARENTERAL IMMUNIZATION WITH FERRITIN

Specific Antibody Levels in the Intestinal Perfusates of <i>Heligosomoides polygyrus</i>-lnfected Mice

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