Dendrimers meet zwitterions: development of a unique antifouling nanoplatform for enhanced blood pool, lymph node and tumor CT imaging

Xiong, Zhijuan; Wang, Yue; Zhu, Jingyi; Li, Xin; He, Yao; Qu, Jinping; Shen, Mingwu; Xia, Jindong; Shi, Xiangyang

doi:10.1039/c7nr03940a

Cited by 54 publications

(82 citation statements)

References 35 publications

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“…Omnipaque). For instance, dendrimers have been employed as templates or stabilizers to synthesize Au NPs for blood pool, major organ, and tumor imaging (Qiao & Shi, 2015 ; Wei et al., 2016 ; Xiong et al., 2017 ). These dendrimer-based Au NPs possess remarkable properties, including improved diagnostic effects, extended blood circulation time, and controllable behaviors in vivo .…”

Section: Introductionmentioning

confidence: 99%

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using ^99mTc-labeled dendrimer-entrapped gold nanoparticles

et al. 2018

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Non-invasive imaging of apoptosis in tumors induced by chemotherapy is of great value in the evaluation of therapeutic efficiency. In this study, we report the synthesis, characterization, and utilization of radionuclide technetium-99m (99mTc)-labeled dendrimer-entrapped gold nanoparticles (Au DENPs) for targeted SPECT/CT imaging of chemotherapy-induced tumor apoptosis. Generation five poly(amidoamine) (PAMAM) dendrimers (G5.NH2) were sequentially conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), polyethylene glycol (PEG) modified duramycin, PEG monomethyl ether, and fluorescein isothiocyanate (FI) to form the multifunctional dendrimers, which were then utilized as templates to entrap gold nanoparticles. Followed by acetylation of the remaining dendrimer surface amines and radiolabeling of 99mTc, the SPECT/CT dual mode nanoprobe of tumor apoptosis was constructed. The developed multifunctional Au DENPs before and after 99mTc radiolabeling were well characterized. The results demonstrate that the multifunctional Au DENPs display favorable colloidal stability under different conditions, own good cytocompatibility in the given concentration range, and can be effectively labeled by 99mTc with high radiochemical stability. Furthermore, the multifunctional nanoprobe enables the targeted SPECT/CT imaging of apoptotic cancer cells in vitro and tumor apoptosis after doxorubicin (DOX) treatment in the established subcutaneous tumor model in vivo. The designed duramycin-functionalized Au DENPs might have the potential to be employed as a nanoplatform for the detection of apoptosis and early tumor response to chemotherapy.

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Section: Introductionmentioning

confidence: 99%

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using ^99mTc-labeled dendrimer-entrapped gold nanoparticles

et al. 2018

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“…Gold NPs were used as regulators to modify the rigidity of the obtained G5·NH 2 -FITC dendrimers. Using sodium borohydride reduction chemistry [ 34 , 40 ], gold NPs were formed with kinds of dendrimer/gold salt molar ratio, such as 1/15, 1/30, 1/45. Thereafter, the remaining terminal amines of the raw product were acetylated, as reported previously [ 41 , 42 ].…”

Section: Methodsmentioning

confidence: 99%

Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers

Liu

Wang

et al. 2018

Pharmaceutics

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Understanding of the interaction between cells and nanoparticles (NPs) is critical. Despite numerous attempts to understand the effect of several parameters of NPs on their cellular uptake behaviors, such as size, shape, surface chemistry, etc., limited information is available regarding NP rigidity. Herein, we investigate the effect of rigidity on cellular uptake behaviors of NPs, using generation 5 poly(amidoamine) dendrimer as a model. By harnessing the abundant inner cavity, their rigidity could be effectively regulated by forming size-tunable gold NPs. The NPs thus formed were well characterized and displayed similar hydrodynamic size, surface potential, fluorescence intensity, and distinct rigidity (owing to differences in the size of the Au core). Flow cytometry analysis revealed a positive correlation between NP rigidity and cellular uptake of NPs. Confocal microscopic evaluation revealed that the entrapped gold NPs may affect the intracellular localization of the internalized dendrimers. The present findings can potentially guide the preparation of suitable NPs for biomedical applications.

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“…The results show that PPI modified with PEG (M w =7,500) has lowest drug release rate and all the PEGylated PPI dendrimers have better blood compatibility than PPI dendrimers without PEGylation. Due Phosphorylcholine [44] CBAA [45,46] Acetyl [42,[46][47][48] CBAA [45,46] RGD [49][50][51][52][53] pAb antibody [54] Lactobionic acid [55] Fluorescein isothiocyanate Fluorescence imaging [55] Fluorochrome Cy5.5 [50] PPI PEG Reducing cytotoxicity [43,56] Maltotriose [57][58][59] Anti-EGFRvlll scFV Targeting property [60] FA [61] Histidine, pyridine, piperazine Buffering capacity property [62] PLL PEG Reducing cytotoxicity [63] FA Targeting property [64] Phosphorous dendrimers Azabisphosphonate Targeting property [65] 8-Anilino-1-naphthalenesulfonate Fluorescence imaging [66] to the optimized steric hindrances of PEGs with M w of 4,000, the designed multifunctional PPI device enables the highest cellular uptake, which is desired for drug delivery applications.…”

Section: Pegylationmentioning

confidence: 99%

Dendrimer-based strategies for cancer therapy: Recent advances and future perspectives

2018

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This review reports some recent advances on the use of dendrimer-based systems for cancer therapy. Dendrimers are emerging as promising carriers or stabilizers for drugs and nanoparticles (NPs) due to their highly branched 3dimensional globular shape, internal hydrophobic cavity and multiple peripheral functional groups. The fabricated nanoplatforms loaded with therapeutic agents such as drugs, siRNAs or NPs can be further modified to have targeting specificity, antifouling properties and good biocompatibility. In particular, recent advances in the surface modifications of dendrimers and the application of dendrimers as versatile platforms for different therapeutic treatments to cancer including chemotherapy, radiotherapy, photothermal therapy, photodynamic therapy, gene therapy, and combination therapy will be introduced in detail.

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Dendrimers meet zwitterions: development of a unique antifouling nanoplatform for enhanced blood pool, lymph node and tumor CT imaging

Cited by 54 publications

References 35 publications

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using ^99mTc-labeled dendrimer-entrapped gold nanoparticles

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using ^99mTc-labeled dendrimer-entrapped gold nanoparticles

Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers

Dendrimer-based strategies for cancer therapy: Recent advances and future perspectives

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Dendrimers meet zwitterions: development of a unique antifouling nanoplatform for enhanced blood pool, lymph node and tumor CT imaging

Cited by 54 publications

References 35 publications

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using 99mTc-labeled dendrimer-entrapped gold nanoparticles

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using 99mTc-labeled dendrimer-entrapped gold nanoparticles

Cellular Uptake Behaviors of Rigidity-Tunable Dendrimers

Dendrimer-based strategies for cancer therapy: Recent advances and future perspectives

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Product

Resources

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SPECT/CT imaging of chemotherapy-induced tumor apoptosis using ^99mTc-labeled dendrimer-entrapped gold nanoparticles

SPECT/CT imaging of chemotherapy-induced tumor apoptosis using ^99mTc-labeled dendrimer-entrapped gold nanoparticles