Dendritic Cell Accumulation in Draining Lymph Nodes during the Induction Phase of Contact Allergy in Mice

Abstract

Draining lymph node cells isolated from mice 24 h following topical exposure to a variety of contact-sensitizing chemicals, including the dinitrobenzene derivatives, 2,4-dinitrochlorobenzene and 2,4-dinitrothiocyanobenzene, contained increased numbers of dendritic cells (DCs). The increase in frequency of DCs was time-dependent and preceded significant changes in either lymph node cellularity or lymph node cell pro-liferative activity. The degree of DC accumulation was also influenced by the chemical used and the concentration employed for sensitization. In the context of contact allergy, the biological relevance of this phenomenon to the induction of hapten-specific responses is indicated by the fact that relatively small numbers of DC-enriched fractions of lymph node cells (comprising approximately 70% DCs), but not unfractionated or DC-depleted populations, transferred sensitization to naive animals. Moreover, using the skin-sensitizing fluorochrome, fluorescein isothiocyanate, it was observed that 24 h following exposure the majority of lymph node cells bearing high concentrations of antigen were within the DC-rich fraction.

B7/CD28 Costimulation Is Critical in Susceptibility toPseudomonas aeruginosaCorneal Infection: A Comparative Study Using Monoclonal Antibody Blockade and CD28-Deficient Mice

“…We were also interested in whether P. aeruginosa Ag associated LC in draining CLN could be detected in our bacterial model. Dependent on the type of tissue in which they reside and the character of the pathogen-related migration-inducing signals, DC have been shown to reach lymph nodes within 4 -48 h (23,(27)(28)(29)(30). The cells can act in the inductive phase of an immune response as well as APC for peripheral tissue-homing memory T cells (24,31).…”

lnterleukin-6 Production by Draining Lymph Node Cells following Primary Contact Sensitisation of Mice: Relationship to the Proliferative Response

“…Following primary exposure to contact sensitising chemicals allergen associates with epidermal Langerhans cells and is transported, via afferent lymphatics, to lymph nodes draining the site of exposure [17]. Presentation of antigen to specific T lymphocytes precedes the induction of proliferation by both antigen-specific and non-specific lymphocytes within the draining lymph node.…”

Correlation between Lymphocyte Proliferative Responses and Dendritic Cell Migration in Regional Lymph Nodes following Skin Painting with Contact-Sensitizing Agents

“…Viable cell counts were performed by exclusion of 0.5% trypan blue, and the concentration was adjusted to 5 x I06 vi able cells/ml. DC-enriched populations were prepared as previ ously described [10]. Briefly LNC (5 ml) were gently layered onto 2 ml of metrizamide (14.5% in RPMI-FCS; Nygaard, Oslo, Nor way) and centrifuged (600 g) for 10 min at room temperature.…”

“…Within hours of skin sensitization DC accumulate in the lymph nodes draining the site of exposure [7][8][9][10], Studies in this and other laboratories have shown that a significant proportion of DC which ar rive in lymph nodes bear high levels of antigen [9][10][11], Such antigen-bearing DC are highly immunocompe tent. They are potent stimulators of primary and sec ondary T lymphocyte proliferation in vitro [9,11,12] and small numbers will efficiently transfer contact sensitization to naive recipients [9,10,13].…”

“…They are potent stimulators of primary and sec ondary T lymphocyte proliferation in vitro [9,11,12] and small numbers will efficiently transfer contact sensitization to naive recipients [9,10,13].…”