Dendritic morphology of presumptive vasoconstrictor and pilomotor neurons and their relations with neuropeptide-containing preganglionic fibres in lumbar sympathetic ganglia of guinea-pigs

Gibbins, Ian L.; Matthew, Sue E.

doi:10.1016/0306-4522(95)00423-8

Cited by 40 publications

(35 citation statements)

References 27 publications

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“…2a-d, 4a). Most of the remaining NK 1 -IR neurons contained TH immunoreactivity but not NPY immunoreactivity 4b): These neurons are mostly pilomotor neurons, and they are not usually surrounded by SP-IR boutons (Gibbins, 1992;Gibbins and Matthew, 1996). Consequently, only Ϸ40% of NK 1 -IR neurons were surrounded by a well-defined basket of SP-IR boutons.…”

Section: Lumbar Chain Gangliamentioning

confidence: 99%

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

1999

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We have used multiple‐labelling immunohistochemistry and confocal microscopy to determine the distribution of immunoreactivity to the tachykinin neurokinin‐1 (NK1) receptors in guinea pig sympathetic ganglia. Although nerve fibres containing immunoreactivity to substance P were common in all ganglia except the superior cervical ganglia, most neurons expressing NK1 receptor immunoreactivity were not closely surrounded by pericellular baskets of substance P‐immunoreactive boutons. Conversely, many neurons surrounded by baskets of substance P‐immunoreactive boutons lacked NK1 immunoreactivity. In the coeliac and inferior mesenteric ganglia, NK1 receptor expression was restricted almost entirely to noradrenergic neurons that contained somatostatin immunoreactivity and projected to the enteric plexuses. In the lumbar chain and paracervical ganglia, NK1 immunoreactivity was expressed by nonnoradrenergic vasodilator neurons containing immunoreactivity to vasoactive intestinal peptide. Taken together, our results show that sympathetic neurons in different functional pathways express NK1 receptor immunoreactivity. However, the neurons that could respond to endogenously released substance P through NK1 receptors may be distant from presynaptic release sites. These observations suggest that, in sympathetic ganglia, substance P may modulate ganglionic transmission through heterosynaptic actions on NK1 receptors. J. Comp. Neurol. 412:693–704, 1999. © 1999 Wiley‐Liss, Inc.

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Section: Lumbar Chain Gangliamentioning

confidence: 99%

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

1999

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“…Triple-labelling immunofluorescence was done as described previously by using a variety of well-characterised antibodies (see Gibbins 1992;Gibbins and Matthew, 1996). Primary antibodies were directed against CGRP (raised in a goat; Arnel, New York, NY; or in a rabbit; Peninsula, Belmont, CA), NPY (raised in a sheep, code E2210, or in a rabbit, code RMJ 264), VIP (raised in a rabbit; Incstar, Stillwater, MN), MERGL (raised in a rabbit; Peninsula), SP (raised in a rat, clone NC1/34HL; SeraLab), tyrosine hydroxylase (TH; raised in a mouse, clone LNC1; Incstar), synapsin (raised in a rabbit; BioGenesis, Bournemouth, UK), synaptophysin (raised in a mouse, clone SY38; Boehringer-Mannheim, Indianapolis, IN), and microtubuleassociated protein-2 (MAP-2; raised in a mouse, clone HM2; Sigma, St. Louis, MO).…”

Section: Multiple-labelling Immunofluorescence and Confocal Microscopymentioning

confidence: 99%

“…The second set of analyses tested our previous observations that terminal boutons of particular populations of peptide-containing preganglionic neurons are associated selectively with specific populations of ganglion cells (Gibbins, 1992;Gibbins and Matthew, 1996). Sections of lumbar ganglia were labelled for immunoreactivity to VIP or NPY (to label cell bodies) and SP, CGRP, or MERGL (to label boutons).…”

Section: Multiple-labelling Immunofluorescence and Confocal Microscopymentioning

confidence: 99%

“…In general, it has been assumed that these baskets represent functional inputs to the neurons that they surround. Some neurons are surrounded by boutons of two different classes of preganglionic neurons, apparently providing direct morphological evidence for the convergence of neurochemically distinct inputs to a single neuron (Gibbins, 1992;Gibbins and Matthew, 1996). However, our recent ultrastructural study of serially sectioned dye-filled neurons from the lumbar sympathetic chain of guinea pigs (Gibbins, 1998;Gibbins et al, unpublished observations) has shown that the total number of synaptic inputs to any particular sympathetic neuron is very low and that the predicted number of boutons forming synapses or close appositions with the cell body is much lower than the number of peptide-containing boutons that form pericellular baskets.…”

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confidence: 94%

“…A feature of the terminals of the peptide-containing preganglionic neurons is that their boutons form characteristic ''pericellular baskets'' around the cell bodies of individual neurons or groups of neurons (e.g., Heym et al, 1984Heym et al, , 1990Lindh et al, 1986;Stofer, 1988, 1989;Yamamoto et al, 1989;Gibbins, 1992;Gibbins and Matthew, 1996). In general, it has been assumed that these baskets represent functional inputs to the neurons that they surround.…”

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Synaptic organisation of neuropeptide-containing preganglionic boutons in lumbar sympathetic ganglia of guinea pigs

et al. 1998

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Within the lumbar sympathetic ganglia of guinea pigs, the endings of different populations of neuropeptide-containing preganglionic neurons form well-defined pericellular baskets of boutons around target neurons in specific functional pathways. We have used multiple-labelling immunofluorescence, confocal microscopy, and ultrastructural immunocytochemistry to investigate synaptic organisation within pericellular baskets labelled for immunoreactivity to calcitonin gene-related peptide (CGRP), substance P (SP), or the pro-enkephalin-derived peptide, met-enkephalin-arg-gly-leu (MERGL) in relation to their target neurons. Different functional populations of neurons, identified by their neurochemical profile, showed a significant degree of spatial clustering and predicted well the distribution of specific classes of pericellular baskets. Most of the boutons in a basket were completely surrounded by Schwann cell processes and did not form synapses. The synapses that were present were made mostly onto dendrites enclosed by the Schwann cell sheath surrounding the neuron within the basket. These dendrites probably originated from neurochemically similar neighbouring neurons. Nevertheless, some of the boutons in the baskets did form synapses with the cell body or proximal dendrites of the neuron they surrounded. Occasionally, cell bodies received a relatively high number of synapses and close appositions from boutons in a pericellular basket. Synaptic convergence of two immunohistochemically distinct types of preganglionic inputs was found in baskets of SP-immunoreactive or MERGL-immunoreactive, but not CGRP-immunoreactive, boutons. Taken together, our results show that the appearance of pericellular baskets is primarily due to the packing of the target neurons. The grouping of functionally similar classes of neurons with their pathway-specific projections of peptide-containing preganglionic neurons suggests that peptides could exert their effects in relatively well-defined zones within the ganglia.

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Neurokinin‐1 receptor localisation in guinea pig autonomic ganglia

Messenger¹,

Anderson²,

Gibbins³

1999

J. Comp. Neurol.

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We have used multiple-labelling immunohistochemistry and confocal microscopy to determine the distribution of immunoreactivity to the tachykinin neurokinin-1 (NK(1)) receptors in guinea pig sympathetic ganglia. Although nerve fibres containing immunoreactivity to substance P were common in all ganglia except the superior cervical ganglia, most neurons expressing NK(1) receptor immunoreactivity were not closely surrounded by pericellular baskets of substance P-immunoreactive boutons. Conversely, many neurons surrounded by baskets of substance P-immunoreactive boutons lacked NK(1) immunoreactivity. In the coeliac and inferior mesenteric ganglia, NK(1) receptor expression was restricted almost entirely to noradrenergic neurons that contained somatostatin immunoreactivity and projected to the enteric plexuses. In the lumbar chain and paracervical ganglia, NK(1) immunoreactivity was expressed by nonnoradrenergic vasodilator neurons containing immunoreactivity to vasoactive intestinal peptide. Taken together, our results show that sympathetic neurons in different functional pathways express NK(1) receptor immunoreactivity. However, the neurons that could respond to endogenously released substance P through NK(1) receptors may be distant from presynaptic release sites. These observations suggest that, in sympathetic ganglia, substance P may modulate ganglionic transmission through heterosynaptic actions on NK(1) receptors.

show abstract

Dendritic morphology of presumptive vasoconstrictor and pilomotor neurons and their relations with neuropeptide-containing preganglionic fibres in lumbar sympathetic ganglia of guinea-pigs

Cited by 40 publications

References 27 publications

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

Synaptic organisation of neuropeptide-containing preganglionic boutons in lumbar sympathetic ganglia of guinea pigs

Neurokinin‐1 receptor localisation in guinea pig autonomic ganglia

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