Dengue Virus–Infected Aedes aegypti in the Home Environment

Garcia-Rejon, Julián E.; Loroño-Pino, María Alba; Farfán-Ale, José A.; Flores-Flores, Luis F.; Rosado-Paredes, Elsy; Rivero-Cárdenas, Nubia; Najera-Vazquez, Rosario; Gómez-Carro, Salvador; Lira-Zumbardo, Victor; González-Martínez, Pedro; Lozano-Fuentes, Saúl; Elizondo-Quiroga, Darwin; Beaty, Barry J.; Eisen, Lars

doi:10.4269/ajtmh.2008.79.940

Cited by 100 publications

(123 citation statements)

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“…Our estimated field infection rates were very high, as expected for viruses like DENV and CHIKV when sampling is focused on the homes of suspected cases with active transmission [32,33]. An important finding was that ZIKV was not detected in C. quinquefasciatus, another common urban tropical mosquito discussed as a potential ZIKV vector [9].…”

Section: Discussionmentioning

confidence: 59%

Outbreak of Zika Virus Infection, Chiapas State, Mexico, 2015, and First Confirmed Transmission byAedes aegyptiMosquitoes in the Americas

et al. 2016

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Background. After decades of obscurity, Zika virus (ZIKV) has spread through the Americas since 2015 accompanied by congenital microcephaly and Guillain-Barré syndrome. Although these epidemics presumably involve transmission by Aedes aegypti, no direct evidence of vector involvement has been reported, prompting speculation that other mosquitoes such as Culex quinquefasciatus could be involved.Methods. We detected an outbreak of ZIKV infection in southern Mexico in late 2015. Sera from suspected ZIKV-infected patients were analyzed for viral RNA and antibodies. Mosquitoes were collected in and around patient homes and tested for ZIKV.Results. Of 119 suspected ZIKV-infected patients, 25 (21%) were confirmed by RT-PCR of serum collected 1-8 days after the onset of signs and symptoms including rash, arthralgia, headache, pruritus, myalgia, and fever. Of 796 mosquitoes collected, A. aegypti yielded ZIKV detection by RT-PCR in 15 of 55 pools (27.3%). No ZIKV was detected in C. quinquefasciatus. ZIKV sequences derived from sera and mosquitoes showed a monophyletic relationship suggestive of a point source introduction from Guatemala.Conclusions. These results demonstrate the continued, rapid northward progression of ZIKV into North America with typically mild disease manifestations, and implicate A. aegypti for the first time as a principal vector in North America.

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Section: Discussionmentioning

confidence: 59%

Outbreak of Zika Virus Infection, Chiapas State, Mexico, 2015, and First Confirmed Transmission byAedes aegyptiMosquitoes in the Americas

et al. 2016

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“…1 Aedes aegypti, the principal urban vector, lives in close contact with humans and commonly transmits the virus to humans in and around homes or other indoor environments. 15,22 Mosquitoes become infected after feeding on a viremic human and after a 4-14 days extrinsic incubation period, the virus can be transmitted to susceptible humans. 23 The mosquito is infected for life and can transmit the virus each time she bites a susceptible human.…”

Section: Introductionmentioning

confidence: 99%

“…All of these field-relevant conditions can result in infectious virus inactivation and/or destruction of viral analytes. 21 The RT-PCR is widely used for detection of arboviruses, including DENV, 22 in field-collected or simulated fieldcollected mosquito pools. The RT-PCR has been shown to *Address correspondence to Barry J. Beaty, Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523.…”

Section: Introductionmentioning

confidence: 99%

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Voge¹,

Sánchez-Vargas²,

Blair³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed individually and pooled with uninfected mosquitoes. Overall, DENV NS1 antigen was detected in 98% of infected mosquitoes/ pools versus 79% for RT-PCR and 29% for virus isolation. Our results indicate that NS1 is an excellent analyte for detection of DENV in Ae. aegypti and that the tested NS1 antigen kit provides a sensitive, rapid, and convenient test for DENV surveillance in mosquitoes.

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“…aegypti that contributes to its efficiency as an epidemiological vector is the close association with domestic habitats (Scott et al, 2000). Adult mosquitoes frequently reside indoors in human dwellings, most commonly in bedrooms (60.3% to 63.5%) followed by living/dining rooms (9.3% to 18.4%), kitchens (7.5% to 9.7%) and bathrooms (6.6% to 11.5%) (García-Rejón et al, 2008;Casas-Martínez, 2013). Immature forms develop primarily in artificial containers such as cans, jars, tires and buckets (Winch et al, 1992;García-Rejón et al, 2011).…”

Section: Behaviour Of Reproductionmentioning

confidence: 99%

“…The relatively large numbers and duration of DENV infected females captured in houses with confirmed dengue cases in Merida, Mexico may further indicate high fidelity between Ae. aegypti mosquitoes and place of pupal emergence (García Rejón et al, 2008).…”

Section: Dispersalmentioning

confidence: 99%

Abbreviations and acronyms

2018

Harmonisation of Regulatory Oversight in Biotechnology

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This work is published under the responsibility of the Secretary-General of the OECD. The opinions expressed and arguments employed herein do not necessarily reflect the official views of OECD member countries.This document, as well as any data and any map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area.Please cite this publication as: OECD (2018) The statistical data for Israel are supplied by and under the responsibility of the relevant Israeli authorities. The use of such data by the OECD is without prejudice to the status of the Golan Heights, East Jerusalem and Israeli settlements in the West Bank under the terms of international law.Photo credits: Cover © Maks Ershov.Corrigenda to OECD publications may be found on line at: www.oecd.org/about/publishing/corrigenda.htm. © OECD 2018You can copy, download or print OECD content for your own use, and you can include excerpts from OECD publications, databases and multimedia products in your own documents, presentations, blogs, websites and teaching materials, provided that suitable acknowledgment of the source and copyright owner(s) is given. All requests for public or commercial use and translation rights should be submitted to rights@oecd.org. Requests for permission to photocopy portions of this material for public or commercial use shall be addressed directly to the Copyright Clearance Center (CCC) at info@copyright.com or the Centre francais d'exploitation du droit de copie (CFC) at contact@cfcopies.com. FOREWORD │ 3SAFETY ASSESSMENTOF TRANSGENIC ORGANISMSIN THE ENVIRONMENT, VOLUME 8 © OECD 2018 ForewordModern biotechnologies are applied to plants species (crops, flowers, trees), animals and micro-organisms. The safety of the resulting transgenic organisms when released in the environment for their use in agriculture, forestry, the food and feed industry or for other applications represents a challenging issue. Genetically engineered products are rigorously assessed by their developers during their elaboration, and by governments when ready for release, to ensure high safety standards. This remains essential with new biotechnology developments using insects to fight against disease outbreaks: engineered mosquitoes need to be evaluated through a scientifically sound approach to risk/safety assessment that will inform biosafety regulators and support the decision concerning the release of these novel organisms in the environment.The OECD offers a long-standing recognised expertise in biosafety and contributes to facilitating an harmonised approach. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology (WG-HROB) was established in 1995. The WG-HROB gathers national authorities responsible for the environmental risk/safety assessment of products of modern biotechnology in OECD countries and other economies. International organisations and experts involved in biosafety activities are a...

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Dengue Virus–Infected Aedes aegypti in the Home Environment

Cited by 100 publications

References 41 publications

Outbreak of Zika Virus Infection, Chiapas State, Mexico, 2015, and First Confirmed Transmission byAedes aegyptiMosquitoes in the Americas

Outbreak of Zika Virus Infection, Chiapas State, Mexico, 2015, and First Confirmed Transmission byAedes aegyptiMosquitoes in the Americas

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

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